Antimicrobial agents such as bavistin, cefotaxime and kanamycin were evaluated for their effects on the rapid shoot regeneration from nodal explants of Centella asiatica (L.). Filter sterilized bavistin (250 mg/L) was...Antimicrobial agents such as bavistin, cefotaxime and kanamycin were evaluated for their effects on the rapid shoot regeneration from nodal explants of Centella asiatica (L.). Filter sterilized bavistin (250 mg/L) was augmented alone and in combination with cytokinins such as BAP and TDZ into the media to trace the effect on regeneration. On this basis, the potential use of bavistin (150 mg/L) along with BAP (2.0 mg/L) was evaluated which showed the maximum shoot number (6.6) and shoot length (4.4 cm) respectively. Cefotaxime at the concentration of 100 μM/L was found to be effective to obtain the maximum shoot number formation (5.8) with the regeneration frequency (90%). Kanamycin at the concentration of 80 μM/L induced maximum shoot regeneration (5.12). Kanamycin at 100 μM/L or at higher concentrations reduced the shoot regeneration. The best rooting response was noticed when in vitro regenerated microshoots were transferred to the rooting medim which was supplemented with IBA (2.0 mg/L). This combination generates 90% of regeneration frequency and maximum number of roots per shoot (14.2) and high root length (4.2 cm). The rooted plants were acclimatized and transferred to field for survivalance. The addition of antibiotics was found to be more effective and safer for using since their effects on regeneration were found to be negligible.展开更多
The mechanism for interactions of the 6-benzyl-amino-purine and its Co2+, Ni2+ complexes with DNA was studied by UV and fluorescence spectra using ethidium bromide (EB) as probe molecule. When pH=7.4, the action of 6-...The mechanism for interactions of the 6-benzyl-amino-purine and its Co2+, Ni2+ complexes with DNA was studied by UV and fluorescence spectra using ethidium bromide (EB) as probe molecule. When pH=7.4, the action of 6-benzyl-amino-purine-Co2+ and DNA was electrostatic effect and certain kind of intercalation action; when pH=9.86, the action of the two was electrostatic effect; when pH=2.85 , the action of 6-benzyl-amino-purine-Ni2+ and DNA was both electrostatic and intercalation action. But when pH=7.4, 6-benzyl-amino-purine hardly influenced the intercalation action of EB and DNA, the action of 6-benzyl-amino-purine and DNA was electrostatic action.展开更多
文摘Antimicrobial agents such as bavistin, cefotaxime and kanamycin were evaluated for their effects on the rapid shoot regeneration from nodal explants of Centella asiatica (L.). Filter sterilized bavistin (250 mg/L) was augmented alone and in combination with cytokinins such as BAP and TDZ into the media to trace the effect on regeneration. On this basis, the potential use of bavistin (150 mg/L) along with BAP (2.0 mg/L) was evaluated which showed the maximum shoot number (6.6) and shoot length (4.4 cm) respectively. Cefotaxime at the concentration of 100 μM/L was found to be effective to obtain the maximum shoot number formation (5.8) with the regeneration frequency (90%). Kanamycin at the concentration of 80 μM/L induced maximum shoot regeneration (5.12). Kanamycin at 100 μM/L or at higher concentrations reduced the shoot regeneration. The best rooting response was noticed when in vitro regenerated microshoots were transferred to the rooting medim which was supplemented with IBA (2.0 mg/L). This combination generates 90% of regeneration frequency and maximum number of roots per shoot (14.2) and high root length (4.2 cm). The rooted plants were acclimatized and transferred to field for survivalance. The addition of antibiotics was found to be more effective and safer for using since their effects on regeneration were found to be negligible.
文摘The mechanism for interactions of the 6-benzyl-amino-purine and its Co2+, Ni2+ complexes with DNA was studied by UV and fluorescence spectra using ethidium bromide (EB) as probe molecule. When pH=7.4, the action of 6-benzyl-amino-purine-Co2+ and DNA was electrostatic effect and certain kind of intercalation action; when pH=9.86, the action of the two was electrostatic effect; when pH=2.85 , the action of 6-benzyl-amino-purine-Ni2+ and DNA was both electrostatic and intercalation action. But when pH=7.4, 6-benzyl-amino-purine hardly influenced the intercalation action of EB and DNA, the action of 6-benzyl-amino-purine and DNA was electrostatic action.
