According to synthetic pathway of plant chlorogenic acid （CGA）, the expression patterns of genes encoding enzymes that are associated with CGA synthesis were studied in normally developed Xuehua pear fruit. The stud...According to synthetic pathway of plant chlorogenic acid （CGA）, the expression patterns of genes encoding enzymes that are associated with CGA synthesis were studied in normally developed Xuehua pear fruit. The study demonstrated that CGA content in peel and flesh of Xuehua pear decreased as fruit development progressed, with a higher level in peel. The expression levels of PbPAL 1, PbPAL2, PbC3H, PbC4H, Pb4CL 1, Pb4CL2, Pb4CL6, PbHC T1 and PbHC T3 genes decreased in fruit, which was consistent with the pattern of variation in CGA content. That indicated that these genes might be key genes for influencing fruit CGA synthesis in Xuehua pear. However, Pb4CL7 gene expression profile is not consistent with variation of CGA content, hence, it may not be a key gene involved in CGA synthesis.展开更多
类黄酮3'-羟化酶(F3'H)在花色素苷生物合成过程中起关键作用。明确多星韭F3'H在花色素苷生物合成中的功能,将为多星韭花色形成及改良研究提供基因资源。基于转录组数据从多星韭花朵中克隆获得AwF3'H,并对其进行生物信...类黄酮3'-羟化酶(F3'H)在花色素苷生物合成过程中起关键作用。明确多星韭F3'H在花色素苷生物合成中的功能,将为多星韭花色形成及改良研究提供基因资源。基于转录组数据从多星韭花朵中克隆获得AwF3'H,并对其进行生物信息学分析,利用Real time PCR对AwF3'H1的表达模式进行分析,并利用蘸花法将AwF3'H转入拟南芥中,同时对转基因植株进行表型观察及花色素苷检测分析。结果表明,Aw F3'H的ORF全长为1545 bp,编码514个氨基酸,属于细胞色素P450家族成员,与洋葱AcF3'H的亲缘关系最近;在所检测组织均有表达,但在雄蕊中表达最高,根中表达最低;与野生型拟南芥相比,转AwF3'H拟南芥T2代幼苗子叶及下胚轴颜色明显加深,矢车菊素与天竺葵素类花色苷积累量显著增加。表明AwF3'H具有类黄酮3'-羟化酶的功能。展开更多
基金supported by the Specific Financial Funds of Hebei Province,China (494-0502-JSN-7FB3)
文摘According to synthetic pathway of plant chlorogenic acid （CGA）, the expression patterns of genes encoding enzymes that are associated with CGA synthesis were studied in normally developed Xuehua pear fruit. The study demonstrated that CGA content in peel and flesh of Xuehua pear decreased as fruit development progressed, with a higher level in peel. The expression levels of PbPAL 1, PbPAL2, PbC3H, PbC4H, Pb4CL 1, Pb4CL2, Pb4CL6, PbHC T1 and PbHC T3 genes decreased in fruit, which was consistent with the pattern of variation in CGA content. That indicated that these genes might be key genes for influencing fruit CGA synthesis in Xuehua pear. However, Pb4CL7 gene expression profile is not consistent with variation of CGA content, hence, it may not be a key gene involved in CGA synthesis.
文摘类黄酮3'-羟化酶(F3'H)在花色素苷生物合成过程中起关键作用。明确多星韭F3'H在花色素苷生物合成中的功能,将为多星韭花色形成及改良研究提供基因资源。基于转录组数据从多星韭花朵中克隆获得AwF3'H,并对其进行生物信息学分析,利用Real time PCR对AwF3'H1的表达模式进行分析,并利用蘸花法将AwF3'H转入拟南芥中,同时对转基因植株进行表型观察及花色素苷检测分析。结果表明,Aw F3'H的ORF全长为1545 bp,编码514个氨基酸,属于细胞色素P450家族成员,与洋葱AcF3'H的亲缘关系最近;在所检测组织均有表达,但在雄蕊中表达最高,根中表达最低;与野生型拟南芥相比,转AwF3'H拟南芥T2代幼苗子叶及下胚轴颜色明显加深,矢车菊素与天竺葵素类花色苷积累量显著增加。表明AwF3'H具有类黄酮3'-羟化酶的功能。
