摘要
赤霉素3β-双氧化酶(GA3ox)是赤霉素(GA)生物合成过程中的关键酶,它通过催化活性GA的形成来影响植物生长发育。为了了解水稻GA3ox家族成员的基本情况,本研究利用生物信息学方法对其家族成员进行了预测、蛋白结构及其聚类分析。结果表明:第一,以OsGA3ox1 (LOC_Os05g08540)作为靶序列,利用BlastP在gramene数据库中共查询到了24个同源基因,同源性为80%～90%;第二,经NCBI在线分析发现,各同源基因的蛋白产物都含有2OG-FeII_Oxy和DIOX_N两个保守结构域,其结构域中都具有与Fe^(2+)结合的2个组氨酸残基(H)和1个天冬氨酸残基(D)以及能结合α-酮戊二酸的1个精氨酸残基(R)和1个丝氨酸残基(S),并且还观察到这些蛋白间具有相同或相似的α-螺旋和β-转角等二级结构,因此,推测它们同属于GA3ox家族;第三,进一步地,利用MEGA7.0构建了该家族的系统进化树,并将该家族分成ClassⅠ和ClassⅡ两个亚族,各亚族分别包括了16个和8个成员。本研究结果为深入研究水稻OsGA3ox基因的生物学功能提供参考依据。
Gibberellin 3β-dioxygenase(GA3ox),a key enzyme involved in the biosynthesis of gibberellin(GAs),can affect the growth and development of plant by catalyzing the formation of active GAs.In order to understand the basic situation of rice GA3ox family members,we used bioinformatic methods to predict its family members,the protein structure and its cluster analysis of rice GA3ox family members were also carried out.The results showed that:(1)Using OsGA3ox1(LOC_Os05g08540)as the target sequence,24 homologous genes were found in the gramene database by BlastP,with homology of 80%~90%.(2)Through NCBI online analysis,it was found that the protein products of all homologous genes contained two conserved domains of 2OG-FeII_Oxy and DIOX_N,each of which contained 2 histidine residues(H)and 1 aspartate residue(D)bound to Fe2+,and 1 arginine residue(R)and 1 serine residue(S)bound toα-oxoglutarate.Moreover,it was also observed that these proteins had the same or similar secondary structure,such asα-helix andβ-angle.Therefore,it was presumed that they might belong to the GA3ox family.(3)The phylogenetic tree of the family was constructed by using the MEGA7.0,and we divided the family into 2 subgroups,class I and class II,each of which included 16 and 8 members,respectively.The results would provided a reference for the further study of the biological functions of OsGA3ox in rice.
作者
殷小林
张超
王有成
袁定阳
谭炎宁
段美娟
Yin Xiaolin;Zhang Chao;Wang Youcheng;Yuan Dingyang;Tan Yanning;Duan Meijuan(Longping Branch,Graduate School of Hunan University,Changsha,410125;Hunan Hybrid Rice Research Center,State Key Laboratory of Hybrid Rice,Changsha,410125;College of Agronomy,Hunan Agricultural University,Changsha 410128,China;Hunan Agricultural University,College of Bioscience and Biotechnology,Changsha,410128)
出处
《分子植物育种》
CAS
CSCD
北大核心
2019年第4期1054-1060,共7页
Molecular Plant Breeding
基金
湖南农业科技创新资金(2017XC09)
国家863课题基金项目(2014AA10A604)共同资助
