Cold stress is a major environmental factor that adversely affects plant growth and development. The C-repeat binding factor/DRE binding factor 1 （CBF/DREB1） transcriptional regulatory cascade has been shown to play...Cold stress is a major environmental factor that adversely affects plant growth and development. The C-repeat binding factor/DRE binding factor 1 （CBF/DREB1） transcriptional regulatory cascade has been shown to play important roles in plant response to cold. Here we demonstrate that two key components of brassinosteroid （BR） signaling modulate freezing tolerance of Arabidopsis plants. The loss-of-function mutant of the GSK3-1ike kinases involved in BR signaling, bin2-3 bill bil2, showed increased freezing tolerance, whereas overexpression of BIN2 resulted in hypersensitivity to freezing stress under both non-acclimated and acclimated conditions. By contrast, gain-of-function mutants of the transcription factors BZR1 and BES1 displayed enhanced freezing tolerance, and consistently cold treatment could induce the accumulation of dephosphorylated BZR1. Biochemical and genetic analyses showed that BZR1 acts upstream of CBF1 and CBF2 to directly regulate their expression. Moreover, we found that BZR1 also regulated other COR genes uncoupled with CBFs, such as WKRY6, PYL6, SOCl, JMT, and SAG21, to modulate plant response to cold stress. Consistently, wrky6 mutants showed decreased freezing tolerance. Taken together, our results indicate that BZR1 positively modulates plant freezing tolerance through CBF-dependent and CBF-independent pathways.展开更多
A recent paper by Kidokoro et al.(2020)in The Plant Cell reported a transgene-dependent transcriptional silencing phenomenon in the dominant ice1-1 Arabidopsis mutant containing the CBF3-LUC reporter,and questioned wh...A recent paper by Kidokoro et al.(2020)in The Plant Cell reported a transgene-dependent transcriptional silencing phenomenon in the dominant ice1-1 Arabidopsis mutant containing the CBF3-LUC reporter,and questioned whether ICE1 may regulate CBF genes and may be involved in plant cold response.Here,we evaluate available evidence supporting the involvement of ICE1 in plant cold response,and provide ChIP-seq data showing ICE1 binding to the promoters of CBF genes and other regulatory genes known to be critical for cold response as well as to the promoters of some COR genes.展开更多
为了解植物对低温胁迫响应机制的研究现状,以“低温胁迫”、“CBF”、“抗寒性”为关键词,在Web of Science核心合集、SpringerLink与中国知网等数据库就2000—2022年已发表的文献进行检索,对研究植物低温胁迫响应机制的相关文献进行总...为了解植物对低温胁迫响应机制的研究现状,以“低温胁迫”、“CBF”、“抗寒性”为关键词,在Web of Science核心合集、SpringerLink与中国知网等数据库就2000—2022年已发表的文献进行检索,对研究植物低温胁迫响应机制的相关文献进行总结和分析,结果表明:1)研究植物对低温胁迫的响应机制有助于为提升植物低温抗性提供依据,对应对全球复杂气候变化有重要意义;2)植物发生低温胁迫时,生物膜系统会遭到损伤,细胞渗透调节物质含量会发生变化以维持正常渗透压,而抗氧化酶的活性一般会呈现先上升后下降的趋势;3)植物有一套复杂信号通路响应机制来抵御低温胁迫,CBF途径可以通过对一些关键蛋白的翻译后修饰来调控植物对低温胁迫的抵抗,在植物抵御低温胁迫时发挥重要作用;4)关于CBF不依赖途径、低温信号感受器和低温信号分子的研究仍有待深入。展开更多
文摘Cold stress is a major environmental factor that adversely affects plant growth and development. The C-repeat binding factor/DRE binding factor 1 （CBF/DREB1） transcriptional regulatory cascade has been shown to play important roles in plant response to cold. Here we demonstrate that two key components of brassinosteroid （BR） signaling modulate freezing tolerance of Arabidopsis plants. The loss-of-function mutant of the GSK3-1ike kinases involved in BR signaling, bin2-3 bill bil2, showed increased freezing tolerance, whereas overexpression of BIN2 resulted in hypersensitivity to freezing stress under both non-acclimated and acclimated conditions. By contrast, gain-of-function mutants of the transcription factors BZR1 and BES1 displayed enhanced freezing tolerance, and consistently cold treatment could induce the accumulation of dephosphorylated BZR1. Biochemical and genetic analyses showed that BZR1 acts upstream of CBF1 and CBF2 to directly regulate their expression. Moreover, we found that BZR1 also regulated other COR genes uncoupled with CBFs, such as WKRY6, PYL6, SOCl, JMT, and SAG21, to modulate plant response to cold stress. Consistently, wrky6 mutants showed decreased freezing tolerance. Taken together, our results indicate that BZR1 positively modulates plant freezing tolerance through CBF-dependent and CBF-independent pathways.
基金supported by the Chinese Academy of Sciences and the Strategic Priority Research Program(Grant No.XDB27040101)of the Chinese Academy of Sciences。
文摘A recent paper by Kidokoro et al.(2020)in The Plant Cell reported a transgene-dependent transcriptional silencing phenomenon in the dominant ice1-1 Arabidopsis mutant containing the CBF3-LUC reporter,and questioned whether ICE1 may regulate CBF genes and may be involved in plant cold response.Here,we evaluate available evidence supporting the involvement of ICE1 in plant cold response,and provide ChIP-seq data showing ICE1 binding to the promoters of CBF genes and other regulatory genes known to be critical for cold response as well as to the promoters of some COR genes.
文摘为了解植物对低温胁迫响应机制的研究现状,以“低温胁迫”、“CBF”、“抗寒性”为关键词,在Web of Science核心合集、SpringerLink与中国知网等数据库就2000—2022年已发表的文献进行检索,对研究植物低温胁迫响应机制的相关文献进行总结和分析,结果表明:1)研究植物对低温胁迫的响应机制有助于为提升植物低温抗性提供依据,对应对全球复杂气候变化有重要意义;2)植物发生低温胁迫时,生物膜系统会遭到损伤,细胞渗透调节物质含量会发生变化以维持正常渗透压,而抗氧化酶的活性一般会呈现先上升后下降的趋势;3)植物有一套复杂信号通路响应机制来抵御低温胁迫,CBF途径可以通过对一些关键蛋白的翻译后修饰来调控植物对低温胁迫的抵抗,在植物抵御低温胁迫时发挥重要作用;4)关于CBF不依赖途径、低温信号感受器和低温信号分子的研究仍有待深入。
