In order to investigate the effect of zedoary glucose injection on reproductive behavior in female mandarin voles(Microtus mandarinus),behavior focal sampling and immunohistochemistry methods were used to examine the ...In order to investigate the effect of zedoary glucose injection on reproductive behavior in female mandarin voles(Microtus mandarinus),behavior focal sampling and immunohistochemistry methods were used to examine the relationships between behavior changes of female mandarin voles and estrogen receptor α immunoreactive neurons (ERα-IRs) in the ovary and in relevant brain regions after being fed with rabbit chow that was mixed with concentrations of 30 mg/kg zedoary glucose injection. Healthy adult female voles were divided into experimental and control groups. The experimental group was fed with the mixed chow and carrot,and control group was fed with rabbit chow and carrot. Behaviors of the adult female voles were observed after being fed for 30 days. The numbers of litters of these adult female voles after mating with adult male voles was recorded,and meanwhile the number of ERα-IRs in the relevant brain regions was detected. The results showed that:during the period of intraspecies female-male interactions in a cage,the female voles in the experimental group displayed more escape behaviors,defensive behaviors,and hostile attacks than did those in the control group. In addition,the number of litters born to the female voles in the experimental group was significantly less than to the control group,and the incubation period of females in the experimental group was significantly prolonged. However,ERα-IR were not significantly different in the ovaries of the female voles in the experimental and control groups,and the number of ERα-IRs in the bed nucleus of the stria terminalis (BST),the medial amygdaloid nucleus (ME),the medial preoptic area (MPO) and the ventromedial hypothalamic (VMH),in the experimental group were significantly less than those in the control group. There was no difference in the arcuate nucleus (ACR). In summary,zedoary glucose injection might affect reproductive behavior of female mandarin voles through changes in the number of the estrogen receptors α (ERα) in relevant brain regions.展开更多
目的:探讨子宫内膜息肉不孕患者增殖期及种植窗期性激素及雌激素受体(ER)和孕激素受体(PR)的改变及意义。方法:选择100例宫腔镜检查见赘生物并经病理证实为子宫内膜息肉(EP)的患者作为EP组,其中,增殖期检查者50例,种植窗期检查者50例。...目的:探讨子宫内膜息肉不孕患者增殖期及种植窗期性激素及雌激素受体(ER)和孕激素受体(PR)的改变及意义。方法:选择100例宫腔镜检查见赘生物并经病理证实为子宫内膜息肉(EP)的患者作为EP组,其中,增殖期检查者50例,种植窗期检查者50例。选择60例已生育宫腔镜检查示宫腔正常并经病理证实者作为对照组,其中,增殖期检查者30例,种植窗期检查者30例。采用化学发光法测定患者血清的性激素水平,免疫组织化学方法检测子宫内膜组织中雌激素受体(ER)、孕激素受体(PR)的表达,并进行比较。结果:EP组血清基础雌二醇(E2)水平显著高于对照组(235.59±109.47 pmol/L vs 159.89±87.48 pmol/L,P<0.05),EP组种植窗期孕酮(P)/E2比值显著低于对照组(0.077 vs 0.090,P<0.05),其他各项性激素指标及ER各组及各期间比较,差异均无统计学意义(P>0.05)。PR在EP组EP组织种植窗期子宫内膜间质中的表达显著低于EP周围正常内膜与对照组(P<0.05)。结论:对于无月经改变的子宫内膜息肉患者,长期较高水平的E2刺激,PR在分泌期EP间质中处于低水平、相对不足状态可能与息肉形成有关。展开更多
文摘In order to investigate the effect of zedoary glucose injection on reproductive behavior in female mandarin voles(Microtus mandarinus),behavior focal sampling and immunohistochemistry methods were used to examine the relationships between behavior changes of female mandarin voles and estrogen receptor α immunoreactive neurons (ERα-IRs) in the ovary and in relevant brain regions after being fed with rabbit chow that was mixed with concentrations of 30 mg/kg zedoary glucose injection. Healthy adult female voles were divided into experimental and control groups. The experimental group was fed with the mixed chow and carrot,and control group was fed with rabbit chow and carrot. Behaviors of the adult female voles were observed after being fed for 30 days. The numbers of litters of these adult female voles after mating with adult male voles was recorded,and meanwhile the number of ERα-IRs in the relevant brain regions was detected. The results showed that:during the period of intraspecies female-male interactions in a cage,the female voles in the experimental group displayed more escape behaviors,defensive behaviors,and hostile attacks than did those in the control group. In addition,the number of litters born to the female voles in the experimental group was significantly less than to the control group,and the incubation period of females in the experimental group was significantly prolonged. However,ERα-IR were not significantly different in the ovaries of the female voles in the experimental and control groups,and the number of ERα-IRs in the bed nucleus of the stria terminalis (BST),the medial amygdaloid nucleus (ME),the medial preoptic area (MPO) and the ventromedial hypothalamic (VMH),in the experimental group were significantly less than those in the control group. There was no difference in the arcuate nucleus (ACR). In summary,zedoary glucose injection might affect reproductive behavior of female mandarin voles through changes in the number of the estrogen receptors α (ERα) in relevant brain regions.
文摘目的:探讨子宫内膜息肉不孕患者增殖期及种植窗期性激素及雌激素受体(ER)和孕激素受体(PR)的改变及意义。方法:选择100例宫腔镜检查见赘生物并经病理证实为子宫内膜息肉(EP)的患者作为EP组,其中,增殖期检查者50例,种植窗期检查者50例。选择60例已生育宫腔镜检查示宫腔正常并经病理证实者作为对照组,其中,增殖期检查者30例,种植窗期检查者30例。采用化学发光法测定患者血清的性激素水平,免疫组织化学方法检测子宫内膜组织中雌激素受体(ER)、孕激素受体(PR)的表达,并进行比较。结果:EP组血清基础雌二醇(E2)水平显著高于对照组(235.59±109.47 pmol/L vs 159.89±87.48 pmol/L,P<0.05),EP组种植窗期孕酮(P)/E2比值显著低于对照组(0.077 vs 0.090,P<0.05),其他各项性激素指标及ER各组及各期间比较,差异均无统计学意义(P>0.05)。PR在EP组EP组织种植窗期子宫内膜间质中的表达显著低于EP周围正常内膜与对照组(P<0.05)。结论:对于无月经改变的子宫内膜息肉患者,长期较高水平的E2刺激,PR在分泌期EP间质中处于低水平、相对不足状态可能与息肉形成有关。
