目的:探讨初产妇孕期体质指数(BMI)变化及孕中期母体血清甲胎蛋白(AFP)、β-人绒毛膜促性腺激素(β-HCG)、游离雌三醇(u E3)水平对妊娠结局的影响。方法:收集513例产前检查并顺利分娩的初产孕妇及新生儿资料,记录孕妇孕期BMI值、孕期检...目的:探讨初产妇孕期体质指数(BMI)变化及孕中期母体血清甲胎蛋白(AFP)、β-人绒毛膜促性腺激素(β-HCG)、游离雌三醇(u E3)水平对妊娠结局的影响。方法:收集513例产前检查并顺利分娩的初产孕妇及新生儿资料,记录孕妇孕期BMI值、孕期检查情况及并发症、分娩方式以及妊娠结局,记录新生儿出生体重,检测孕妇(孕14~20周)空腹静脉血清AFP、uE3、β-HCG水平,观察初产妇孕期BMI及血清娠AFP、u E3、β-HCG水平与不良妊娠结局的相关性。结果:孕期BMI增长过多与剖宫产、妊娠期高血压、巨大儿、新生儿窒息、妊娠期糖尿病均呈正相关(χ~2=17.170、17.110、17.318、12.870、10.835,P<0.05),血清AFP>2.0 Mo M与胎盘早剥、胎膜早破、胎儿窘迫、妊娠期高血压均呈正相关(r=0.458、0.362、0.735、0.466,P<0.05),β-HCG>2.0 Mo M与胎盘早剥、胎膜早破、妊娠期高血压、胎儿窘迫、妊娠期糖尿病均呈正相关(r=0.202、0.218、0.368、1.000、0.488,P<0.05),u E3<0.7 Mo M与妊娠期高血压、胎儿窘迫、胎膜早破、胎盘早剥均呈正相关(r=0.490、0.712、0.754、0.684,P<0.05)。结论:初产妇孕期BMI值增加过多,以及孕中期AFP、β-HCG水平过高和u E3水平过低会增加不良妊娠结局风险。展开更多
It is difficult to determine estrogens directly by electrochemical methods because estradiol, estrone and estriol are hydrophobic and electroinactive. Thus, they are only determined by the indirect electrochemical tec...It is difficult to determine estrogens directly by electrochemical methods because estradiol, estrone and estriol are hydrophobic and electroinactive. Thus, they are only determined by the indirect electrochemical techniques. In this paper, based on the voltammetric behavior of estrogens(estradiol, estrone and estriol) on the Nafion\|modified glassy carbon electrode in the presence of CTAB, a novel direct electroanalytical method for the determination of the total amounts of estradiol, estrone and estriol was developed. In pH=8.00 phosphate buffer and in the presence of 6×10\+\{-5\} mol/L CTAB, estradiol, estrone and estriol have very sensitive oxidation peaks at 0.38 V(\%vs\%. SCE) at Nafion\|modified glassy carbon electrode after being accumulated for 3 min at -0.50 V. This peak can be used to determine trace level of estradiol, estrone and estriol. It was found that estradol, estrone and estriol have the similar electrochemical responses on the Nafion\|modified electrode in the presence of other long\|chain cationic surfactants, such as CPB, STAB, CATAB, LTAB. It is observed that the enhancement effect is highest in the presence of CTAB, and the peak current is CTAB concentration\|dependent. However, in the presence of long\|chain anionic surfactants, such as SDBS, and long\|chain nonionic surfactants, such as Triton X\|100, estradiol, estrone and estriol have no voltammetric responses on the Nafion\|modified glassy carbon electrode. The mechanism for the oxidation of estradiol in this system has been discussed, and the reactant site for the oxidation of estradiol has been verified to be the hyrdoxyl group in A\|ring.展开更多
文摘目的:探讨初产妇孕期体质指数(BMI)变化及孕中期母体血清甲胎蛋白(AFP)、β-人绒毛膜促性腺激素(β-HCG)、游离雌三醇(u E3)水平对妊娠结局的影响。方法:收集513例产前检查并顺利分娩的初产孕妇及新生儿资料,记录孕妇孕期BMI值、孕期检查情况及并发症、分娩方式以及妊娠结局,记录新生儿出生体重,检测孕妇(孕14~20周)空腹静脉血清AFP、uE3、β-HCG水平,观察初产妇孕期BMI及血清娠AFP、u E3、β-HCG水平与不良妊娠结局的相关性。结果:孕期BMI增长过多与剖宫产、妊娠期高血压、巨大儿、新生儿窒息、妊娠期糖尿病均呈正相关(χ~2=17.170、17.110、17.318、12.870、10.835,P<0.05),血清AFP>2.0 Mo M与胎盘早剥、胎膜早破、胎儿窘迫、妊娠期高血压均呈正相关(r=0.458、0.362、0.735、0.466,P<0.05),β-HCG>2.0 Mo M与胎盘早剥、胎膜早破、妊娠期高血压、胎儿窘迫、妊娠期糖尿病均呈正相关(r=0.202、0.218、0.368、1.000、0.488,P<0.05),u E3<0.7 Mo M与妊娠期高血压、胎儿窘迫、胎膜早破、胎盘早剥均呈正相关(r=0.490、0.712、0.754、0.684,P<0.05)。结论:初产妇孕期BMI值增加过多,以及孕中期AFP、β-HCG水平过高和u E3水平过低会增加不良妊娠结局风险。
文摘It is difficult to determine estrogens directly by electrochemical methods because estradiol, estrone and estriol are hydrophobic and electroinactive. Thus, they are only determined by the indirect electrochemical techniques. In this paper, based on the voltammetric behavior of estrogens(estradiol, estrone and estriol) on the Nafion\|modified glassy carbon electrode in the presence of CTAB, a novel direct electroanalytical method for the determination of the total amounts of estradiol, estrone and estriol was developed. In pH=8.00 phosphate buffer and in the presence of 6×10\+\{-5\} mol/L CTAB, estradiol, estrone and estriol have very sensitive oxidation peaks at 0.38 V(\%vs\%. SCE) at Nafion\|modified glassy carbon electrode after being accumulated for 3 min at -0.50 V. This peak can be used to determine trace level of estradiol, estrone and estriol. It was found that estradol, estrone and estriol have the similar electrochemical responses on the Nafion\|modified electrode in the presence of other long\|chain cationic surfactants, such as CPB, STAB, CATAB, LTAB. It is observed that the enhancement effect is highest in the presence of CTAB, and the peak current is CTAB concentration\|dependent. However, in the presence of long\|chain anionic surfactants, such as SDBS, and long\|chain nonionic surfactants, such as Triton X\|100, estradiol, estrone and estriol have no voltammetric responses on the Nafion\|modified glassy carbon electrode. The mechanism for the oxidation of estradiol in this system has been discussed, and the reactant site for the oxidation of estradiol has been verified to be the hyrdoxyl group in A\|ring.
