Transgenic tobacco plants carrying Cry1Ac, Cry1Ie or both genes were obtained. In the leaves of transgenic plants carrying both genes, the contents of Cry1Ac and Cry1Ie proteins were 0.173% and 0.131% of the total pro...Transgenic tobacco plants carrying Cry1Ac, Cry1Ie or both genes were obtained. In the leaves of transgenic plants carrying both genes, the contents of Cry1Ac and Cry1Ie proteins were 0.173% and 0.131% of the total proteins, respectively. Cry1Ac protein content was 0.182% and Cry1Ie protein con- tent was 0.124% of the total proteins in the leaves of transgenic plants containing only one Bt gene. Fresh leaves of transgenic tobacco and wild-type plants were used for the insect bioassay against wild-type and Cry1Ac-resistant cotton bollworm (Helicoverpa armigera). The bioassay results showed that transgenic plants carrying both genes were significantly more toxic to wild-type and Cry1Ac-resistant cotton bollworm than those carrying Cry1Ac or Cry1Ie alone. This study indicates that the higher toxicity of transgenic tobacco plants carrying both genes is caused by the cooperative function of both Bt proteins, thus providing a potential way to delay the development of insect resis- tance to transgenic crops.展开更多
目的:构建简便可用的^(225)Ac-^(213)Bi发生器,为研发靶向α治疗药物提供短半衰期α核素^(213)Bi。方法:以DIPEX树脂作为发生器固定相,采用Sep-Pak Alumina N Plus Light Cartridge小柱作为柱体构建发生器,以盐酸作为淋洗液。使用γ能...目的:构建简便可用的^(225)Ac-^(213)Bi发生器,为研发靶向α治疗药物提供短半衰期α核素^(213)Bi。方法:以DIPEX树脂作为发生器固定相,采用Sep-Pak Alumina N Plus Light Cartridge小柱作为柱体构建发生器,以盐酸作为淋洗液。使用γ能谱对淋洗所得的^(213)Bi进行纯度分析,验证使用活度计对^(225)Ac与^(213)Bi活度进行测量的方法。研究不同浓度盐酸溶液及不同保存状态下发生器的洗脱曲线,确定最佳使用方案。结果:使用商品化^(225)Ac、100 mg DIPEX树脂和Sep-Pak Alumina N Plus Light Cartridge小柱构建柱体积为0.35 ml的^(225)Ac-^(213)Bi发生器,通过注射盐酸淋洗获得^(213)Bi;获得^(213)Bi的γ能谱纯净,测定半衰期为(45.75±0.12)min,与理论值(45.6 min)一致。CRC^(R)活度计刻度号#086和#018可用于测量^(225)Ac和^(213)Bi活度,平均测量误差分别为-2.6%和+0.2%。^(225)Ac-^(213)Bi发生器使用2 mol/L盐酸作为淋洗液,320μl淋洗体积可获得90%洗脱率;发生器应在淋洗溶液中保存,避免干燥。结论:构建的^(225)Ac-^(213)Bi发生器可制备^(213)Bi用于靶向α治疗。展开更多
基金Supported by the National Plant Transformation and Industrialization Program of China (Grant No. JY03-A-13)
文摘Transgenic tobacco plants carrying Cry1Ac, Cry1Ie or both genes were obtained. In the leaves of transgenic plants carrying both genes, the contents of Cry1Ac and Cry1Ie proteins were 0.173% and 0.131% of the total proteins, respectively. Cry1Ac protein content was 0.182% and Cry1Ie protein con- tent was 0.124% of the total proteins in the leaves of transgenic plants containing only one Bt gene. Fresh leaves of transgenic tobacco and wild-type plants were used for the insect bioassay against wild-type and Cry1Ac-resistant cotton bollworm (Helicoverpa armigera). The bioassay results showed that transgenic plants carrying both genes were significantly more toxic to wild-type and Cry1Ac-resistant cotton bollworm than those carrying Cry1Ac or Cry1Ie alone. This study indicates that the higher toxicity of transgenic tobacco plants carrying both genes is caused by the cooperative function of both Bt proteins, thus providing a potential way to delay the development of insect resis- tance to transgenic crops.
文摘目的:构建简便可用的^(225)Ac-^(213)Bi发生器,为研发靶向α治疗药物提供短半衰期α核素^(213)Bi。方法:以DIPEX树脂作为发生器固定相,采用Sep-Pak Alumina N Plus Light Cartridge小柱作为柱体构建发生器,以盐酸作为淋洗液。使用γ能谱对淋洗所得的^(213)Bi进行纯度分析,验证使用活度计对^(225)Ac与^(213)Bi活度进行测量的方法。研究不同浓度盐酸溶液及不同保存状态下发生器的洗脱曲线,确定最佳使用方案。结果:使用商品化^(225)Ac、100 mg DIPEX树脂和Sep-Pak Alumina N Plus Light Cartridge小柱构建柱体积为0.35 ml的^(225)Ac-^(213)Bi发生器,通过注射盐酸淋洗获得^(213)Bi;获得^(213)Bi的γ能谱纯净,测定半衰期为(45.75±0.12)min,与理论值(45.6 min)一致。CRC^(R)活度计刻度号#086和#018可用于测量^(225)Ac和^(213)Bi活度,平均测量误差分别为-2.6%和+0.2%。^(225)Ac-^(213)Bi发生器使用2 mol/L盐酸作为淋洗液,320μl淋洗体积可获得90%洗脱率;发生器应在淋洗溶液中保存,避免干燥。结论:构建的^(225)Ac-^(213)Bi发生器可制备^(213)Bi用于靶向α治疗。
