目的探讨α-硫辛酸联合甲钴胺治疗糖尿病周围神经病变的临床疗效及可能机制。方法收集2013年3月—2014年3月解放军三二三医院收治的患者188例,随机分为对照组和治疗组,每组94例。两组均给予常规治疗。对照组将甲钴胺1.0 mg加入250 m L...目的探讨α-硫辛酸联合甲钴胺治疗糖尿病周围神经病变的临床疗效及可能机制。方法收集2013年3月—2014年3月解放军三二三医院收治的患者188例,随机分为对照组和治疗组,每组94例。两组均给予常规治疗。对照组将甲钴胺1.0 mg加入250 m L生理盐水中静脉滴注,1次/d。治疗组在对照组的基础上静脉滴注注射用硫辛酸,0.45 g加入生理盐水250 m L中,1次/d。两组均连续治疗3周。比较两组的周围神经传导速度、缺氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)、多伦多临床评分(TCSS)。结果与对照组相比,治疗组胫前神经的运动神经传导速度(MNCV)、感觉神经传导速度(SNCV)和腓总神经的MNCV提高更为显著,差异具有统计学意义(P<0.05)。治疗后治疗组HIF-1α和VEGF、对照组HIF-1α均明显降低,与治疗前相比差异具有统计学意义(P<0.01);与对照组相比,治疗组HIF-1α和VEGF变化更为显著,差异具有统计学意义(P<0.01)。治疗后两组的TCSS评分均明显降低,与治疗前相比差异具有统计学意义(P<0.01),与对照组相比,治疗组下降更为显著,两组比较差异具有统计学意义(P<0.01)。结论α-硫辛酸联合甲钴胺能够提高糖尿病周围神经病变的临床疗效,其作用机制可能与其诱导HIF-1α表达有关。展开更多
AIM: To investigate the expression and significance of PTEN,hypoxia-inducible factor-1 alpha (HIF-1α), and targeting gene VEGF during colorectal carciogenesis.METHODS: Total 71 cases colorectal neoplasms (9 cases of ...AIM: To investigate the expression and significance of PTEN,hypoxia-inducible factor-1 alpha (HIF-1α), and targeting gene VEGF during colorectal carciogenesis.METHODS: Total 71 cases colorectal neoplasms (9 cases of colorectal adenoma and 62 colorectal adenocarcinoma)were formalin fixed and paraffin-embedded, and all specimens were evaluated for PTEN mRNA, HIF-1α mRNA and VEGF protein expression. PTEN mRNA, HIF-1α mRNA were detected by in situ hybridization. VEGF protein was identified by citrate-microwave SP immunohistochemical method.RESULTS: There were significant differences in PTEN, HIF1α and VEGF expression between colorectal adenomas and colorectal adenocarcinoma (P＜0.05). The level of PTEN expression decreased as the pathologic stage increased.Conversely, HIF-1α and VEGF expression increased with the Dukes stage as follows: stage A (0.1029±0.0457:0.1207± 0.0436), stage B (0.1656±0.0329: 0.1572±0.0514),and stage C+D (0.2335±0.0748: 0.2219±0.0803). For PTEN expression, there was a significant difference among Dukes stage A, B, and C+D, and the level of PTEN expression was found to be significant higher in Dukes stage A or B than that of Dukes stage C or D. For HIF-1α expression,there was a significant difference between Dukes stage A and B, and the level of HIF-1α expression was found to be significantly higher in Dukes stage C+D than that of Dukes stage A or B. The VEGF expression had similar results as HIF-1α expression. In colorectal adenocarcinoma,decreased levels of PTEN were significantly associated with increased expression of HIF-1α mRNA (r=-0.36, P＜0.05)and VEGF protein (r=-0.48, P＜0.05) respectively. The levels of HIF-1 were positively correlated with VEGF expression (r=0.71, P＜0.01).CONCLUSION: Loss of PTEN expression and increased levels of HIF-1α and VEGF may play an important role in carcinogenesis and progression of colorectal adenocarcinoma.展开更多
文摘目的探讨α-硫辛酸联合甲钴胺治疗糖尿病周围神经病变的临床疗效及可能机制。方法收集2013年3月—2014年3月解放军三二三医院收治的患者188例,随机分为对照组和治疗组,每组94例。两组均给予常规治疗。对照组将甲钴胺1.0 mg加入250 m L生理盐水中静脉滴注,1次/d。治疗组在对照组的基础上静脉滴注注射用硫辛酸,0.45 g加入生理盐水250 m L中,1次/d。两组均连续治疗3周。比较两组的周围神经传导速度、缺氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)、多伦多临床评分(TCSS)。结果与对照组相比,治疗组胫前神经的运动神经传导速度(MNCV)、感觉神经传导速度(SNCV)和腓总神经的MNCV提高更为显著,差异具有统计学意义(P<0.05)。治疗后治疗组HIF-1α和VEGF、对照组HIF-1α均明显降低,与治疗前相比差异具有统计学意义(P<0.01);与对照组相比,治疗组HIF-1α和VEGF变化更为显著,差异具有统计学意义(P<0.01)。治疗后两组的TCSS评分均明显降低,与治疗前相比差异具有统计学意义(P<0.01),与对照组相比,治疗组下降更为显著,两组比较差异具有统计学意义(P<0.01)。结论α-硫辛酸联合甲钴胺能够提高糖尿病周围神经病变的临床疗效,其作用机制可能与其诱导HIF-1α表达有关。
文摘AIM: To investigate the expression and significance of PTEN,hypoxia-inducible factor-1 alpha (HIF-1α), and targeting gene VEGF during colorectal carciogenesis.METHODS: Total 71 cases colorectal neoplasms (9 cases of colorectal adenoma and 62 colorectal adenocarcinoma)were formalin fixed and paraffin-embedded, and all specimens were evaluated for PTEN mRNA, HIF-1α mRNA and VEGF protein expression. PTEN mRNA, HIF-1α mRNA were detected by in situ hybridization. VEGF protein was identified by citrate-microwave SP immunohistochemical method.RESULTS: There were significant differences in PTEN, HIF1α and VEGF expression between colorectal adenomas and colorectal adenocarcinoma (P＜0.05). The level of PTEN expression decreased as the pathologic stage increased.Conversely, HIF-1α and VEGF expression increased with the Dukes stage as follows: stage A (0.1029±0.0457:0.1207± 0.0436), stage B (0.1656±0.0329: 0.1572±0.0514),and stage C+D (0.2335±0.0748: 0.2219±0.0803). For PTEN expression, there was a significant difference among Dukes stage A, B, and C+D, and the level of PTEN expression was found to be significant higher in Dukes stage A or B than that of Dukes stage C or D. For HIF-1α expression,there was a significant difference between Dukes stage A and B, and the level of HIF-1α expression was found to be significantly higher in Dukes stage C+D than that of Dukes stage A or B. The VEGF expression had similar results as HIF-1α expression. In colorectal adenocarcinoma,decreased levels of PTEN were significantly associated with increased expression of HIF-1α mRNA (r=-0.36, P＜0.05)and VEGF protein (r=-0.48, P＜0.05) respectively. The levels of HIF-1 were positively correlated with VEGF expression (r=0.71, P＜0.01).CONCLUSION: Loss of PTEN expression and increased levels of HIF-1α and VEGF may play an important role in carcinogenesis and progression of colorectal adenocarcinoma.
