摘要
AIM: To investigate the expression and significance of PTEN,hypoxia-inducible factor-1 alpha (HIF-1α), and targeting gene VEGF during colorectal carciogenesis.METHODS: Total 71 cases colorectal neoplasms (9 cases of colorectal adenoma and 62 colorectal adenocarcinoma)were formalin fixed and paraffin-embedded, and all specimens were evaluated for PTEN mRNA, HIF-1α mRNA and VEGF protein expression. PTEN mRNA, HIF-1α mRNA were detected by in situ hybridization. VEGF protein was identified by citrate-microwave SP immunohistochemical method.RESULTS: There were significant differences in PTEN, HIF1α and VEGF expression between colorectal adenomas and colorectal adenocarcinoma (P＜0.05). The level of PTEN expression decreased as the pathologic stage increased.Conversely, HIF-1α and VEGF expression increased with the Dukes stage as follows: stage A (0.1029±0.0457:0.1207± 0.0436), stage B (0.1656±0.0329: 0.1572±0.0514),and stage C+D (0.2335±0.0748: 0.2219±0.0803). For PTEN expression, there was a significant difference among Dukes stage A, B, and C+D, and the level of PTEN expression was found to be significant higher in Dukes stage A or B than that of Dukes stage C or D. For HIF-1α expression,there was a significant difference between Dukes stage A and B, and the level of HIF-1α expression was found to be significantly higher in Dukes stage C+D than that of Dukes stage A or B. The VEGF expression had similar results as HIF-1α expression. In colorectal adenocarcinoma,decreased levels of PTEN were significantly associated with increased expression of HIF-1α mRNA (r=-0.36, P＜0.05)and VEGF protein (r=-0.48, P＜0.05) respectively. The levels of HIF-1 were positively correlated with VEGF expression (r=0.71, P＜0.01).CONCLUSION: Loss of PTEN expression and increased levels of HIF-1α and VEGF may play an important role in carcinogenesis and progression of colorectal adenocarcinoma.
AIM;To investigate the expression and significance of P-FEN, hypoxia-inducible factor-1 alpha(HIF-1α),and targeting gene VEGF during colorectal carciogenesis. METHODS:Total 71 cases colorectal neoplasms(9 cases of colorectal adenoma and 62 colorectal adenocarcinoma) were formalin fixed and paraffin-embedded,and all specimens were evaluated for PTEN mRNA,HIF-1α mRNA and VEGF protein expression.PTEN mRNA,HIF-1α mRNA were detected by in situ hybridization.VEGF protein was identified by citrate-microwave SP immunohistochemical method. RESULTS:There were significant differences in PTEN,HIF- 1α and VEGF expression between colorectal adenomas and colorectal adenocarcinoma(P<0.05).The level of PTEN expression decreased as the pathologic stage increased. Conversely,HIF-1α and VEGF expression increased with the Dukes stage as follows:stage A(0.1029±0.0457: 0.1207±0.0436),stage B(0.1656±0.0329:0.1572±0.0514), and stage C+D(0.2335±0.0748:0.2219±0.0803).For PTEN expression,there was a significant difference among Dukes stage A,B,and C+D,and the level of PTEN expression was found to be significant higher in Dukes stage A or B than that of Dukes stage C or D.For HIF-1α expression, there was a significant difference between Dukes stage A and B,and the level of HIF-1α expression was found to be significantly higher in Dukes stage C+D than that of Dukes stage A or B.The VEGF expression had similar results as HIF-1α expression.In colorectal adenocarcinoma, decreased levels of PTEN were significantly associated with increased expression of HIF-1α mRNA(r=-0.36,P<0.05) and VEGF protein(r=-0.48,P<0.05)respectively.The levels of HIF-1 were positively correlated with VEGF expression (r=0.71,P<0.01)CONCLUSION: Loss of PTEN expression and increased levels of HIF-la and VEGF may play an important role in carcinogenesis and progression of colorectal adenocarcinoma.
