AIM： To investigate the pattern of mucin expression and concentration in bile obtained during endoscopic retrograde cholangiography （ERC） in relation to gallstone disease.METHODS： Bile samples obtained at ERC from...AIM： To investigate the pattern of mucin expression and concentration in bile obtained during endoscopic retrograde cholangiography （ERC） in relation to gallstone disease.METHODS： Bile samples obtained at ERC from 29 consecutive patients, 17 with and 12 without gallstone disease were evaluated for mucin content by gel filtration on a Sepharose CL-4B column. Dot blot analysis for bile mucin apoproteins was performed with antibodies to Mucin 1 （MUC1）, MUC2, MUC3, MUC5AC, MUC5B and MUC6. Staining intensity score （0-3） was used as a measure of antigen expression.RESULTS： MUCl, MUC2, MUC3, MUCSAC, MUC5B and MUC6 were demonstrated in 34.4%, 34.4%, 51.7%, 51.7%, 55.1% and 27.5% of bile samples, respectively.The staining intensity scores were 0.62 ± 0.94, 0.58 ± 0.90, 0.79 ± 0.97, 1.06 ± 1.22, 1.20 ± 2.26 and 0.41 ± 0.73, respectively. Mean mucin concentration measured in bile by the Sepharose CL-4B method was 22.8 ± 24.0 mg/mL （range 3.4-89.0 mg/mL）. Mean protein concentration was 8.1 ± 4.8 mg/mL （range 1.7-23.2 mg/mL）.CONCLUSION： High levels of MUC3, MUC5AC and MUC5B are expressed in bile aspirated during ERC examination. A specific pattern of mucin gene expression or change in mucin concentration was not found in gallstone disease.展开更多
目的探讨益髓生血颗粒含药血清对K562细胞向红系分化的影响。方法用SD大鼠制备益髓生血颗粒含药血清,分高、中、低和对照组,各组含药血清以20%终浓度添加到1640培养基中,并对K562细胞进行干预培养。采用联苯胺染色法观察各组血清干预K56...目的探讨益髓生血颗粒含药血清对K562细胞向红系分化的影响。方法用SD大鼠制备益髓生血颗粒含药血清,分高、中、低和对照组,各组含药血清以20%终浓度添加到1640培养基中,并对K562细胞进行干预培养。采用联苯胺染色法观察各组血清干预K562细胞后,对蓝染细胞进行计数,计算细胞蓝染阳性率;采用实时荧光定量聚合酶链反应(real time polymerase chain reaction,RT-PCR)方法观察益髓生血颗粒干预K562细胞后珠蛋白基因mRNA表达情况。结果联苯胺染色结果显示高、中、低、对照组与空白组比较均能够显著提高K562细胞蓝染率(P<0.0001);与对照组比较,益髓生血颗粒含药血清高、中剂量组K562细胞蓝染数量提高(P<0.001)。RT-PCR结果显示益髓生血颗粒含药血清,高剂量组与对照组比较,能够促进α和γ-珠蛋白基因表达(P<0.05)。结论益髓生血颗粒含药血清能够促进K562细胞向红系分化。展开更多
文摘AIM： To investigate the pattern of mucin expression and concentration in bile obtained during endoscopic retrograde cholangiography （ERC） in relation to gallstone disease.METHODS： Bile samples obtained at ERC from 29 consecutive patients, 17 with and 12 without gallstone disease were evaluated for mucin content by gel filtration on a Sepharose CL-4B column. Dot blot analysis for bile mucin apoproteins was performed with antibodies to Mucin 1 （MUC1）, MUC2, MUC3, MUC5AC, MUC5B and MUC6. Staining intensity score （0-3） was used as a measure of antigen expression.RESULTS： MUCl, MUC2, MUC3, MUCSAC, MUC5B and MUC6 were demonstrated in 34.4%, 34.4%, 51.7%, 51.7%, 55.1% and 27.5% of bile samples, respectively.The staining intensity scores were 0.62 ± 0.94, 0.58 ± 0.90, 0.79 ± 0.97, 1.06 ± 1.22, 1.20 ± 2.26 and 0.41 ± 0.73, respectively. Mean mucin concentration measured in bile by the Sepharose CL-4B method was 22.8 ± 24.0 mg/mL （range 3.4-89.0 mg/mL）. Mean protein concentration was 8.1 ± 4.8 mg/mL （range 1.7-23.2 mg/mL）.CONCLUSION： High levels of MUC3, MUC5AC and MUC5B are expressed in bile aspirated during ERC examination. A specific pattern of mucin gene expression or change in mucin concentration was not found in gallstone disease.
文摘目的探讨益髓生血颗粒含药血清对K562细胞向红系分化的影响。方法用SD大鼠制备益髓生血颗粒含药血清,分高、中、低和对照组,各组含药血清以20%终浓度添加到1640培养基中,并对K562细胞进行干预培养。采用联苯胺染色法观察各组血清干预K562细胞后,对蓝染细胞进行计数,计算细胞蓝染阳性率;采用实时荧光定量聚合酶链反应(real time polymerase chain reaction,RT-PCR)方法观察益髓生血颗粒干预K562细胞后珠蛋白基因mRNA表达情况。结果联苯胺染色结果显示高、中、低、对照组与空白组比较均能够显著提高K562细胞蓝染率(P<0.0001);与对照组比较,益髓生血颗粒含药血清高、中剂量组K562细胞蓝染数量提高(P<0.001)。RT-PCR结果显示益髓生血颗粒含药血清,高剂量组与对照组比较,能够促进α和γ-珠蛋白基因表达(P<0.05)。结论益髓生血颗粒含药血清能够促进K562细胞向红系分化。
