目的:3,4′,5-三羟基芪-3-β-单-D-葡萄糖苷(Polydatin,Pol)对兔颈动脉内皮损伤性血栓形成的影响.方法:采用胰蛋白酶损伤兔颈动脉内皮诱导血栓形成模型.结果:Pol iv 5或10 mg·kg^(-1)均可显著减少血栓湿重(P<0.05,P<0.01)并...目的:3,4′,5-三羟基芪-3-β-单-D-葡萄糖苷(Polydatin,Pol)对兔颈动脉内皮损伤性血栓形成的影响.方法:采用胰蛋白酶损伤兔颈动脉内皮诱导血栓形成模型.结果:Pol iv 5或10 mg·kg^(-1)均可显著减少血栓湿重(P<0.05,P<0.01)并抑制血小板聚集(P<0.05,P<0.01).Pol 0.30—1.15 mmol·L^(-1)抑制血小板TXA_2生成(P<0.05,P<0.01),不影响人脐静脉内皮细胞生成PGI_2.结论:Pol具有抗血栓作用;不减少PGI_2,选择性抑制TXA_2是其重要机制之一.展开更多
The production and characterization of a monoclonal antibody (MAb AB10) against GA 3 glucoside as well as GA 3 is described. MAb AB10 was derived from an immunogen in which human serum albumin (HSA) was linked to G...The production and characterization of a monoclonal antibody (MAb AB10) against GA 3 glucoside as well as GA 3 is described. MAb AB10 was derived from an immunogen in which human serum albumin (HSA) was linked to GA 3 at carbon 3. This antibody showed high affinity for GA 3 glucoside as well as for 13 hydroxy gibberellins (GA 1, GA 3, GA 5, etc). The affinity of MAb AB10 for 13 hydroxy GAs was significantly reduced by methylation of the 7 oic acid but not by glycosylation of 3 hydroxyl group. Based on this antibody, both of competitive enzyme linked immunosorbent assays (ELISAs) for GA 3 glucoside and for GA 3 were developed. These two ELISAs displayed linear detection ranges from 0 2 pmol to 20 pmol. Using these assays, the fluctuation of GA 3 like and GA 3 glucoside like substances in the leaves of Rumex japonicus was investigated. The results indicated that the glycosylation of free GAs was connected with leaf senescence and that the function of 6 benzyl amino purine in retarding the leaf senescence was probably related to delaying the process of glycosylation of free GAs.展开更多
文摘The production and characterization of a monoclonal antibody (MAb AB10) against GA 3 glucoside as well as GA 3 is described. MAb AB10 was derived from an immunogen in which human serum albumin (HSA) was linked to GA 3 at carbon 3. This antibody showed high affinity for GA 3 glucoside as well as for 13 hydroxy gibberellins (GA 1, GA 3, GA 5, etc). The affinity of MAb AB10 for 13 hydroxy GAs was significantly reduced by methylation of the 7 oic acid but not by glycosylation of 3 hydroxyl group. Based on this antibody, both of competitive enzyme linked immunosorbent assays (ELISAs) for GA 3 glucoside and for GA 3 were developed. These two ELISAs displayed linear detection ranges from 0 2 pmol to 20 pmol. Using these assays, the fluctuation of GA 3 like and GA 3 glucoside like substances in the leaves of Rumex japonicus was investigated. The results indicated that the glycosylation of free GAs was connected with leaf senescence and that the function of 6 benzyl amino purine in retarding the leaf senescence was probably related to delaying the process of glycosylation of free GAs.
