The effect of exotic plants on Bacillus diversity in the rhizosphere and the role of Bacilli in exotic or native plant species remain poorly understood.Flaveria bidentis is an invasive grass in China.Setaria viridis i...The effect of exotic plants on Bacillus diversity in the rhizosphere and the role of Bacilli in exotic or native plant species remain poorly understood.Flaveria bidentis is an invasive grass in China.Setaria viridis is a native grass and occurs in areas invaded by F.bidentis.Our objectives were(i)to examine the differences in the Bacillus communities between F.bidentis and S.viridis rhizospheres soil,and(ii)to compare the effects of Bacilli from F.bidentis and S.viridis rhizospheres on the competitiveness of the invasive species.Flaveria bidentis monoculture,mixture of F.bidentis and S.viridis and S.viridis monoculture were designed in the field experiment.Bacillus diversity in their rhizosphere was analyzed using 16S rRNA.One of the dominant Bacilli in the rhizosphere soil of F.bidentis was selected to test its effect on the competitive growth of F.bidentis in a greenhouse experiment.Bacillus diversity differed in F.bidentis and S.viridis rhizosphere.Brevibacterium frigoritolerans was the dominant Bacilli in the rhizosphere of both F.bidentis and S.viridis;however,its relative abundance in the F.bidentis rhizosphere was much higher than that in the S.viridis rhizosphere.In addition,B.frigoritolerans in the F.bidentis rhizosphere enhanced the growth of the plant compared with that of S.viridis by improving the nitrogen and phosphorus levels.This study showed that F.bidentis invasion influenced Bacillus communities,especially B.frigoritolerans,which,in turn,facilitated F.bidentis growth by increasing the levels of available nitrogen and phosphorus.展开更多
文摘以水霉菌(Saprolegnia ferax)的生物防治为切入点,通过对本实验室保存的9株芽孢杆菌(Bacillus spp.)与水霉菌JL菌株进行对峙拮抗试验,筛选出具有明显抑制水霉菌JL菌株的芽孢杆菌BA1(P<0.05)。经进一步测定菌株BA1对不同来源水霉菌株试验,结果显示菌株BA1对水霉菌株6#、10#、S2也有明显的抑制作用(P<0.05)。通过测定菌株BA1的16 S rDNA,利用BLAST软件进行同源性比对,发现与蜡样芽孢杆菌的同源性在99%以上。由此推测,菌株BA1为蜡样芽孢杆菌菌株,且具有明显抑制水霉菌作用,可以作为水产养殖上水霉病防治的生物制剂使用。
基金funded by the National Natural Science Foundation of China(31972343)Hebei National Natural Science Foundation(C2019201059)College of Life Science,Institute of Life Science and Green Development,Hebei University.
文摘The effect of exotic plants on Bacillus diversity in the rhizosphere and the role of Bacilli in exotic or native plant species remain poorly understood.Flaveria bidentis is an invasive grass in China.Setaria viridis is a native grass and occurs in areas invaded by F.bidentis.Our objectives were(i)to examine the differences in the Bacillus communities between F.bidentis and S.viridis rhizospheres soil,and(ii)to compare the effects of Bacilli from F.bidentis and S.viridis rhizospheres on the competitiveness of the invasive species.Flaveria bidentis monoculture,mixture of F.bidentis and S.viridis and S.viridis monoculture were designed in the field experiment.Bacillus diversity in their rhizosphere was analyzed using 16S rRNA.One of the dominant Bacilli in the rhizosphere soil of F.bidentis was selected to test its effect on the competitive growth of F.bidentis in a greenhouse experiment.Bacillus diversity differed in F.bidentis and S.viridis rhizosphere.Brevibacterium frigoritolerans was the dominant Bacilli in the rhizosphere of both F.bidentis and S.viridis;however,its relative abundance in the F.bidentis rhizosphere was much higher than that in the S.viridis rhizosphere.In addition,B.frigoritolerans in the F.bidentis rhizosphere enhanced the growth of the plant compared with that of S.viridis by improving the nitrogen and phosphorus levels.This study showed that F.bidentis invasion influenced Bacillus communities,especially B.frigoritolerans,which,in turn,facilitated F.bidentis growth by increasing the levels of available nitrogen and phosphorus.
