Four populations of Reed voles (Microtus fortis) were used to check genetic polymorphisms of the mitochondrial DNA (mtDNA) D-loop by means of RFLP analysis. The PCR fragments of this region were digested with 15 restr...Four populations of Reed voles (Microtus fortis) were used to check genetic polymorphisms of the mitochondrial DNA (mtDNA) D-loop by means of RFLP analysis. The PCR fragments of this region were digested with 15 restriction endonucleases: 10 with no variation and the other five including Eco T22I,Eco RV,Hap II,Hind III and Xba I, indicating polymorphisms among different individuals. Ten haplotypes were eventually recovered among sampled 40 individuals. The genetic divergence among these haplotypes ranged respectively from 0.0014 to 0.0168. All vole samples clustered into two distinct branches: Voles from Dongting Lake and some voles from Qingtongxia comprised one branch, and voles from Heilongjiang and the rests from Qingtongxia comprised the other branch. These findings suggested that the mtDNA D-loop region in this species provides informative variations for population research and phylogeography in the future.展开更多
为探讨羚牛分类学地位,应用聚合酶链式反应(PCR)扩增了羚牛、绵羊、山羊和斑羚(青羊)线粒体DNA(mtDNA)细胞色素b基因(Cyt b gene),并测序,结合GenBank检索序列,对9种偶蹄类动物、1种奇蹄类动物Cyt b gene序列差异进行分析,构建了分子系...为探讨羚牛分类学地位,应用聚合酶链式反应(PCR)扩增了羚牛、绵羊、山羊和斑羚(青羊)线粒体DNA(mtDNA)细胞色素b基因(Cyt b gene),并测序,结合GenBank检索序列,对9种偶蹄类动物、1种奇蹄类动物Cyt b gene序列差异进行分析,构建了分子系统树(最优NJ树和唯一MP树)。通过本研究分析表明羚牛与羊亚科动物亲缘关系最近,将羚牛归入羊亚科较为合理。展开更多
文摘Four populations of Reed voles (Microtus fortis) were used to check genetic polymorphisms of the mitochondrial DNA (mtDNA) D-loop by means of RFLP analysis. The PCR fragments of this region were digested with 15 restriction endonucleases: 10 with no variation and the other five including Eco T22I,Eco RV,Hap II,Hind III and Xba I, indicating polymorphisms among different individuals. Ten haplotypes were eventually recovered among sampled 40 individuals. The genetic divergence among these haplotypes ranged respectively from 0.0014 to 0.0168. All vole samples clustered into two distinct branches: Voles from Dongting Lake and some voles from Qingtongxia comprised one branch, and voles from Heilongjiang and the rests from Qingtongxia comprised the other branch. These findings suggested that the mtDNA D-loop region in this species provides informative variations for population research and phylogeography in the future.
文摘为探讨羚牛分类学地位,应用聚合酶链式反应(PCR)扩增了羚牛、绵羊、山羊和斑羚(青羊)线粒体DNA(mtDNA)细胞色素b基因(Cyt b gene),并测序,结合GenBank检索序列,对9种偶蹄类动物、1种奇蹄类动物Cyt b gene序列差异进行分析,构建了分子系统树(最优NJ树和唯一MP树)。通过本研究分析表明羚牛与羊亚科动物亲缘关系最近,将羚牛归入羊亚科较为合理。
