摘要
目的探讨姜黄素致人结肠癌HT-29细胞线粒体DNA(mtDNA)和核DNA(nDNA)损伤作用。方法人结肠癌HT-29细胞暴露于不同质量浓度的姜黄素(0.0,2.5,5.0,10.020.0μg/ml)2h后,采用长链PCR方法分别扩增mtDNA和nDNA片段,琼脂糖凝胶电泳检测扩增产物量。结果mtDNA扩增产物在5.0μg/ml姜黄素作用下明显下降,在5.0,10.020.0μg/ml作用下mtDNA损伤频率分别为(1.23±0.10),(1.97±0.24)和(5.78±0.62)/10kb,与对照组比较,差异均有统计学意义(P<0.01);nDNA扩增产物在20.0μg/ml姜黄素作用下才明显下降,在5.0,10.0,20.0μg/ml作用下nDNA损伤频率分别为(0.27±0.04),(0.61±0.11)和(1.61±0.12)/10kb,与对照组比较,除5.0μg/ml作用组外差异均有统计学意义(P<0.01)。结论姜黄素对HT-29细胞的mtDNA和nDNA损伤均呈剂量依赖关系,并且对mtDNA的损伤作用明显大于nDNA。
Objective To compare the damage effects of cummin on mitochondrial DNA （mtDNA） and nuclear DNA （nDNA） in human colon cancer cell line HT-29. Methods After HT-29 cells were exposed to various concentrations of curcumin（ 0,2.5,5.0,10. 0,20.0μg/ml） for 2h,high molecular weight DNA was isolated and long polymerase chain reaction （LPCR） assay was performed to determine the damage of mtDNA and nDNA. Results A 2h exposure of HT-29 cells to curcumin led to a dose-dependent decrease in the amplification of both mtDNA and nDNA. Loss of amplification of the mtDNA occurred at a lower dose of curcumin （5μg/ml） compared with the nDNA （ 101.Lg/ml）. For mtDNA,DNA lesion frequencies for groups with different concentrations of curcumin（5, 10 and 20μg/ml） were 1.23 ±0. 10,1.97 ±0. 24 and 5.78 ± 0. 62 lesions/10kb （ P 〈 0.01 ）, and that of for nDNA were 0. 27 ± 0. 04,0. 61 ± 0. 11 and 1.61 ± 0. 12 lesions/10kb, respectively. Conclusion In HT-29 cells,curcumin induces DNA damage to both mtDNA and nDNA, and the damage to mtDNA is more serious as compared with nDNA.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2009年第6期665-666,共2页
Chinese Journal of Public Health
基金
国家自然科学基金(30771820)
