自然条件下绝缘子表面的污秽成分受地形、气候及绝缘子表面材料的影响。为有效测量和研究电力系统外绝缘表面污秽成分,研究了离子交换色谱法对绝缘子表面污秽成分的具体测量方法,并对重庆地区自然积污的瓷、玻璃、复合绝缘子表面自然污...自然条件下绝缘子表面的污秽成分受地形、气候及绝缘子表面材料的影响。为有效测量和研究电力系统外绝缘表面污秽成分,研究了离子交换色谱法对绝缘子表面污秽成分的具体测量方法,并对重庆地区自然积污的瓷、玻璃、复合绝缘子表面自然污秽离子成分进行了测量。针对目前普遍采用的离子配对原则的不足,提出了基于模糊聚类的离子配对原则,为污秽成分离子配对提供了依据。研究结果表明:重庆地区的输电线路绝缘子表面主要污秽成分为CaSO_4;由于污秽成分在不同材料表面的积累特性不同,位于同一地点3种绝缘子表面污秽成分和含量分布有所差异;等值盐密(equivalent salt deposit density,ESDD)无法表征电力系统外绝缘自然积污程度,需在污秽地区建立基于污秽成分的污秽程度表征方法,以提高电力系统外绝缘污秽测试的可靠性。展开更多
An effective cation-exchange chromatographic method for lysozyme isolation from chicken egg white is presented, using supermacroporous cryogel grafted with sulfo functional groups. The chromatographic processes were c...An effective cation-exchange chromatographic method for lysozyme isolation from chicken egg white is presented, using supermacroporous cryogel grafted with sulfo functional groups. The chromatographic processes were carried out by one-step and sequential elution, respectively. Sodium phosphate buffer （pH 7.8） containing different concentrations of NaC1 is used as elution agent. The corresponding breakthrough characteristics and elution behaviors in the cryogel bed were investigated and analyzed. Purity of lysozyme in the elution effluent was assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）. The maximum purity of the obtained lysozyme was about 96%, and the cryogel is demonstrated as a potential separation medium for purification of high-purit lysozyme from chicken egg white.展开更多
文摘自然条件下绝缘子表面的污秽成分受地形、气候及绝缘子表面材料的影响。为有效测量和研究电力系统外绝缘表面污秽成分,研究了离子交换色谱法对绝缘子表面污秽成分的具体测量方法,并对重庆地区自然积污的瓷、玻璃、复合绝缘子表面自然污秽离子成分进行了测量。针对目前普遍采用的离子配对原则的不足,提出了基于模糊聚类的离子配对原则,为污秽成分离子配对提供了依据。研究结果表明:重庆地区的输电线路绝缘子表面主要污秽成分为CaSO_4;由于污秽成分在不同材料表面的积累特性不同,位于同一地点3种绝缘子表面污秽成分和含量分布有所差异;等值盐密(equivalent salt deposit density,ESDD)无法表征电力系统外绝缘自然积污程度,需在污秽地区建立基于污秽成分的污秽程度表征方法,以提高电力系统外绝缘污秽测试的可靠性。
基金Supported by the National lqatural Science Foundation of China （21036005, 20876145）, the Science and Technology Cooperation Project between China-Europe Country＇s Governments from the Ministry of Science and Technology of China （1017） and the Natural Science Foundation of Zhejiang Provincial （Y4080326）.
文摘An effective cation-exchange chromatographic method for lysozyme isolation from chicken egg white is presented, using supermacroporous cryogel grafted with sulfo functional groups. The chromatographic processes were carried out by one-step and sequential elution, respectively. Sodium phosphate buffer （pH 7.8） containing different concentrations of NaC1 is used as elution agent. The corresponding breakthrough characteristics and elution behaviors in the cryogel bed were investigated and analyzed. Purity of lysozyme in the elution effluent was assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）. The maximum purity of the obtained lysozyme was about 96%, and the cryogel is demonstrated as a potential separation medium for purification of high-purit lysozyme from chicken egg white.
