目的：观察白子菜对胰岛素抵抗人肝癌HepG2细胞的胰岛素受体（insulin receptor，InsR），葡萄糖转运蛋白4（glucosetransporter4，GLUT4）mRNA表达和蛋白激酶B（proteinkinaseB，PKB），糖原合成激酶-3／3（glycogensynthasekinase-3β...目的：观察白子菜对胰岛素抵抗人肝癌HepG2细胞的胰岛素受体（insulin receptor，InsR），葡萄糖转运蛋白4（glucosetransporter4，GLUT4）mRNA表达和蛋白激酶B（proteinkinaseB，PKB），糖原合成激酶-3／3（glycogensynthasekinase-3β，GSK一3／3）蛋白表达的影响，探讨其干预胰岛素抵抗的分子机制。方法：用胰岛素诱导HepG2细胞使其产生胰岛素抵抗，实验分为空白组，模型组，自子菜水提物（Gynura divaricata hot water extracts，GDE）高、低质量浓度（1．0，0．5g·L^-1）组，白子菜总黄酮（G．divaricataflavonoids，GDF）高、低质量浓度（0．2，0．1g·L^-1）组，白子菜总生物碱（G．divaricata alkaloid，GDA）高、低质量浓度（0．1，0．05g·L^-1）组，二甲双胍（1×10^-3mol·L^-1）组，葡萄糖氧化酶法检测HepG2细胞培养液上清液中葡萄糖的含量，实时荧光定量聚合酶反应（Real-timePCR）检测HepG2细胞InsR，GLUT4mRNA表达，蛋白免疫印迹法（Western blot）检测HepG2细胞PKB，GSK-3β的蛋白表达。结果：与模型组比较，1．0，0．5g·L。GDE组上清液葡萄糖含量极显著降低（P〈0．01），0．2g·L^-1GDF组上清液葡萄糖含量显著降低（P〈0．05）；GDE，GDF组InsR，GLUT4的mRNA表达显著增加（P〈0．05）；GDE组PKB的蛋白表达显著增加（P〈0．05），GSK-3β的蛋白表达显著降低（P〈0．05），GDF组PKB的蛋白表达极显著增加（P〈0．01），GSK-3β的蛋白表达极显著降低（P〈0．01）。结论：白子菜可改善胰岛素抵抗HepG2细胞对葡萄糖的摄取，其机制可能与上调胰岛素抵抗HepG2细胞InsR，GLUT4mRNA表达和PKB的蛋白表达及降低GSK-3β的蛋白表达相关。展开更多
The dynamic change of total flavonoids content in above-ground part of Gynura bicolor (Willd. ) DC. and G. divaricata (L. ) DC. from two locations was researched. The result shows that the total flavonoids content in ...The dynamic change of total flavonoids content in above-ground part of Gynura bicolor (Willd. ) DC. and G. divaricata (L. ) DC. from two locations was researched. The result shows that the total flavonoids content in G. bicolor and G. divaricata leaf is lower in June and July,higher in the beginning and the middle of August and then has a certain decrease with a great fluctuation. Total flavonoids content in leaf of G. bicolor reaches the highest with a value of 12.031 mg·g-1 at the 15th August,and that in leaf of G. divaricata-1(from Zhejiang of China) and G. divaricata-2 (from South America) is the highest at the 1st August with a value of 17.381 and 19.935 mg·g-1,respectively. The total flavonoids content in leaf of G. bicolor and G. divaricata is higher than that in stem. Except leaf of G. divaricata-2,the total flavonoids content in stem and leaf appears decreasing trend from upper to lower part gradually. Therefore,taking total flavonoids content for an index,it is suitable to harvest upper leaf of G. bicolor,upper leaf and stem of G. divaricata-1,and whole leaf of G. divaricata-2 in August.展开更多
文摘目的：观察白子菜对胰岛素抵抗人肝癌HepG2细胞的胰岛素受体（insulin receptor，InsR），葡萄糖转运蛋白4（glucosetransporter4，GLUT4）mRNA表达和蛋白激酶B（proteinkinaseB，PKB），糖原合成激酶-3／3（glycogensynthasekinase-3β，GSK一3／3）蛋白表达的影响，探讨其干预胰岛素抵抗的分子机制。方法：用胰岛素诱导HepG2细胞使其产生胰岛素抵抗，实验分为空白组，模型组，自子菜水提物（Gynura divaricata hot water extracts，GDE）高、低质量浓度（1．0，0．5g·L^-1）组，白子菜总黄酮（G．divaricataflavonoids，GDF）高、低质量浓度（0．2，0．1g·L^-1）组，白子菜总生物碱（G．divaricata alkaloid，GDA）高、低质量浓度（0．1，0．05g·L^-1）组，二甲双胍（1×10^-3mol·L^-1）组，葡萄糖氧化酶法检测HepG2细胞培养液上清液中葡萄糖的含量，实时荧光定量聚合酶反应（Real-timePCR）检测HepG2细胞InsR，GLUT4mRNA表达，蛋白免疫印迹法（Western blot）检测HepG2细胞PKB，GSK-3β的蛋白表达。结果：与模型组比较，1．0，0．5g·L。GDE组上清液葡萄糖含量极显著降低（P〈0．01），0．2g·L^-1GDF组上清液葡萄糖含量显著降低（P〈0．05）；GDE，GDF组InsR，GLUT4的mRNA表达显著增加（P〈0．05）；GDE组PKB的蛋白表达显著增加（P〈0．05），GSK-3β的蛋白表达显著降低（P〈0．05），GDF组PKB的蛋白表达极显著增加（P〈0．01），GSK-3β的蛋白表达极显著降低（P〈0．01）。结论：白子菜可改善胰岛素抵抗HepG2细胞对葡萄糖的摄取，其机制可能与上调胰岛素抵抗HepG2细胞InsR，GLUT4mRNA表达和PKB的蛋白表达及降低GSK-3β的蛋白表达相关。
文摘The dynamic change of total flavonoids content in above-ground part of Gynura bicolor (Willd. ) DC. and G. divaricata (L. ) DC. from two locations was researched. The result shows that the total flavonoids content in G. bicolor and G. divaricata leaf is lower in June and July,higher in the beginning and the middle of August and then has a certain decrease with a great fluctuation. Total flavonoids content in leaf of G. bicolor reaches the highest with a value of 12.031 mg·g-1 at the 15th August,and that in leaf of G. divaricata-1(from Zhejiang of China) and G. divaricata-2 (from South America) is the highest at the 1st August with a value of 17.381 and 19.935 mg·g-1,respectively. The total flavonoids content in leaf of G. bicolor and G. divaricata is higher than that in stem. Except leaf of G. divaricata-2,the total flavonoids content in stem and leaf appears decreasing trend from upper to lower part gradually. Therefore,taking total flavonoids content for an index,it is suitable to harvest upper leaf of G. bicolor,upper leaf and stem of G. divaricata-1,and whole leaf of G. divaricata-2 in August.
