AIM: To study the effect of blocking intestinal lymphatic circulation in two-hit rats and explore the significance of intestinal lymphatic circulation in two-hit. METHODS: Wistar rats were divided equally into three g...AIM: To study the effect of blocking intestinal lymphatic circulation in two-hit rats and explore the significance of intestinal lymphatic circulation in two-hit. METHODS: Wistar rats were divided equally into three groups: mesenteric lymph duct ligation group, non- ligation group and sham group. Mesenteric lymph was diverted by ligation of mesenteric lymph duct, and the two-hit model was established by hemorrhage and lipopolysaccharide (LPS) methods. All rats were sampled for serum pre-experiment and 24 h post- experiment. The organs including kidney, liver, lung and heart were collected for pathomorphologic observation and biochemical investigation. The nitric oxide (NO), malondialdehyde (MDA) and superoxide dismutase (SOD) were determined in serum and tissue homogenate. RESULTS: Pathomorphology study showed that the structures of kidney, lung, liver and heart tissues were normal in sham group; congestion, degeneration and necrosis in non-ligation group; but only mild lesions in ligation group. After two-hits, the contents of AST, ALT, BUN, Cr and LDH-1 in the serum of non-ligation group and ligation group were obviously higher than that in pre-experiment group and sham group, but obviously lower than that in non-ligation group. The contents of NO2-/NO3-, NOS, iNOS and MDA in the serum of non- ligation group were significantly increased, compared with pre-experiment and sham group, but SOD was significantly lower. These parameters were significantly different in ligation group compared with that in sham group, but NO2-/NO3-, iNOS and MDA in ligation group were significantly lower than that in non-ligation group. CONCLUSION: Ligation of mesenteric lymph duct could improve the disturbance of organic function and morphologic damage in two-hit rats; the lymphatic mechanism in two-hit should be emphasized.展开更多
乳腺癌已成为全球女性中最常见的恶性肿瘤,手术治疗是乳腺癌综合治疗的重要手段之一,以乳腺癌改良根治术应用最广,但术中肌肉、筋膜等组织损伤,淋巴循环系统的破坏会影响患侧上肢功能,易致关节挛缩、肌力减弱、僵硬麻木、疼痛等继而出...乳腺癌已成为全球女性中最常见的恶性肿瘤,手术治疗是乳腺癌综合治疗的重要手段之一,以乳腺癌改良根治术应用最广,但术中肌肉、筋膜等组织损伤,淋巴循环系统的破坏会影响患侧上肢功能,易致关节挛缩、肌力减弱、僵硬麻木、疼痛等继而出现患肢功能受限[1],影响患者的生活质量,造成患者身体和心理负担。快速康复理念(en-hanced recovery after surgery,ERAS)核心内容是基于循证建议来优化和促进患者术后恢复的一系列措施[2]。在我国从2001年在结直肠癌患者手术治疗过程的首次应用到骨科、妇科、乳腺外科的围手术期,都取得了满意的临床效果。展开更多
乳腺癌相关淋巴水肿(breast cancer related lymphedema,BCRL)是乳腺癌术后最严重的并发症之一,主要原因是手术破坏淋巴引流系统,导致上肢、腋窝和肩部淋巴循环代偿功能降低,上肢淋巴回流继发性障碍,最终引起淋巴水肿[1]。目前,针对乳...乳腺癌相关淋巴水肿(breast cancer related lymphedema,BCRL)是乳腺癌术后最严重的并发症之一,主要原因是手术破坏淋巴引流系统,导致上肢、腋窝和肩部淋巴循环代偿功能降低,上肢淋巴回流继发性障碍,最终引起淋巴水肿[1]。目前,针对乳腺癌术后上肢淋巴水肿有多种治疗方法,主要目的是减轻症状,防止上肢功能进一步退化[2]。展开更多
基金Supported by National Science Foundation of China, No. 30370561Natural Science Foundation of Hebei Province, No. C2004000649
文摘AIM: To study the effect of blocking intestinal lymphatic circulation in two-hit rats and explore the significance of intestinal lymphatic circulation in two-hit. METHODS: Wistar rats were divided equally into three groups: mesenteric lymph duct ligation group, non- ligation group and sham group. Mesenteric lymph was diverted by ligation of mesenteric lymph duct, and the two-hit model was established by hemorrhage and lipopolysaccharide (LPS) methods. All rats were sampled for serum pre-experiment and 24 h post- experiment. The organs including kidney, liver, lung and heart were collected for pathomorphologic observation and biochemical investigation. The nitric oxide (NO), malondialdehyde (MDA) and superoxide dismutase (SOD) were determined in serum and tissue homogenate. RESULTS: Pathomorphology study showed that the structures of kidney, lung, liver and heart tissues were normal in sham group; congestion, degeneration and necrosis in non-ligation group; but only mild lesions in ligation group. After two-hits, the contents of AST, ALT, BUN, Cr and LDH-1 in the serum of non-ligation group and ligation group were obviously higher than that in pre-experiment group and sham group, but obviously lower than that in non-ligation group. The contents of NO2-/NO3-, NOS, iNOS and MDA in the serum of non- ligation group were significantly increased, compared with pre-experiment and sham group, but SOD was significantly lower. These parameters were significantly different in ligation group compared with that in sham group, but NO2-/NO3-, iNOS and MDA in ligation group were significantly lower than that in non-ligation group. CONCLUSION: Ligation of mesenteric lymph duct could improve the disturbance of organic function and morphologic damage in two-hit rats; the lymphatic mechanism in two-hit should be emphasized.
文摘乳腺癌已成为全球女性中最常见的恶性肿瘤,手术治疗是乳腺癌综合治疗的重要手段之一,以乳腺癌改良根治术应用最广,但术中肌肉、筋膜等组织损伤,淋巴循环系统的破坏会影响患侧上肢功能,易致关节挛缩、肌力减弱、僵硬麻木、疼痛等继而出现患肢功能受限[1],影响患者的生活质量,造成患者身体和心理负担。快速康复理念(en-hanced recovery after surgery,ERAS)核心内容是基于循证建议来优化和促进患者术后恢复的一系列措施[2]。在我国从2001年在结直肠癌患者手术治疗过程的首次应用到骨科、妇科、乳腺外科的围手术期,都取得了满意的临床效果。
文摘乳腺癌相关淋巴水肿(breast cancer related lymphedema,BCRL)是乳腺癌术后最严重的并发症之一,主要原因是手术破坏淋巴引流系统,导致上肢、腋窝和肩部淋巴循环代偿功能降低,上肢淋巴回流继发性障碍,最终引起淋巴水肿[1]。目前,针对乳腺癌术后上肢淋巴水肿有多种治疗方法,主要目的是减轻症状,防止上肢功能进一步退化[2]。
