<Abstract>Background and Objective:Cytokine-induced killer(CIK) cells and autologous dendritic cells-CIK(DCCIK) cells co-cultured with autologous dendritic cells(DCs) and CIK cells are commonly used for immunoth...<Abstract>Background and Objective:Cytokine-induced killer(CIK) cells and autologous dendritic cells-CIK(DCCIK) cells co-cultured with autologous dendritic cells(DCs) and CIK cells are commonly used for immunotherapy recently.We compared the anti-tumor immune response of CIK cells,autologous DC-CIK cells,and semi-allogeneic DC-CIK cells to explore a more effective anti-tumor adoptive immunotherapy approach.Met hods:Peripheral monocytes were isolated from patients with renal carcinoma,lung cancer,or maxillary squamous cell carcinoma and their healthy adult children.Isolated cells were cultured and induced as DCs and CIK cells in vitro.CIK cells from patients were co-cultured with autologous DCs and DCs from their children respectively,generating DC-CIK cells and semi-allogeneic DC-CIK cells.The anti-tumor activities of autologous CIK cells,autologous DC-CIK cells,and semi-allogeneic DC-CIK cells were measured by LDH assay.Intracellular staining was used to test the secretion of cytokines.Flow cytometry was applied for detecting the phonotype changes of these three types of cells.Cell proliferation and cell apoptosis were detected by 5,6carboxyfluorescein diacetate succinimidyl ester(CFSE) and Annexin V/PI respectively.Result s:Compared with autologous CIK cells and DC-CIK cells,semi-allogeneic DC-CIK cells significantly enhanced the anti-tumor activity and IFN-γ secretion,reduced IL-4 secretion,increased the ratio of CD3+CD56+ cells and CD3+CD8+ cells,decreased the number of CD4+CD25+ cells,promoted cell proliferation,and lessened cell apoptosis.Conclusions:Semi-allogeneic DC-CIK cells had a stronger anti-tumor effect than did autologous CIK cells and DC-CIK cells.Our results provided experimental evidence for clinical application of DC-CIK cells.展开更多
目的通过体外试验研究黄芪多糖(Astragalus mongholicus,ASP)对树突状细胞(dendrit- ic cells,DC)功能调节的机制,为进一步阐明黄芪多糖的免疫学活性提供依据。方法应用流式细胞仪检测技术、扫描电镜技术、酶联免疫吸附试验检测DC表型...目的通过体外试验研究黄芪多糖(Astragalus mongholicus,ASP)对树突状细胞(dendrit- ic cells,DC)功能调节的机制,为进一步阐明黄芪多糖的免疫学活性提供依据。方法应用流式细胞仪检测技术、扫描电镜技术、酶联免疫吸附试验检测DC表型和功能的各种指标。结果本实验应用小鼠骨髓来源的DC,通过体外试验证明了黄芪多糖能够提高DC表面分子CD11c和MHCⅡ的表达,并且呈黄芪多糖浓度依赖性;空白组DC的吞噬功能很强,LPS组DC和黄芪多糖处理组DC吞噬功能都明显下降;黄芪多糖能够促进DC白细胞介素-12(IL-12)的表达;电镜观察DC的超微结构,可见黄芪多糖处理组DC突起增多,形态上更加成熟。结论本实验结果证实了黄芪多糖能促进小鼠骨髓来源的DC表型及功能的成熟。展开更多
OBJECTIVE: To investigate the effect of thorascopic administration of ginseng polysaccharides(GPS)plus dendritic cells(DC) on T helper cell type 1/T helper cell type 2(Th1/Th2) balance in patients with non-small cell ...OBJECTIVE: To investigate the effect of thorascopic administration of ginseng polysaccharides(GPS)plus dendritic cells(DC) on T helper cell type 1/T helper cell type 2(Th1/Th2) balance in patients with non-small cell lung cancer(NSCLC).METHODS: A total of 96 NSCLC patients were divided evenly into two groups. The control group was treated with DCs alone and the treatment group was treated with DCs plus GPS. After DCs and GPS were administered thoracoscopically, once a week,4 times for 30 days, the patients' quality of life was measured with the Functional Assessment of Can-cer Treatment-Lung(FACT-L) questionnaire before and after treatment. Serum interferon-γ(INF-γ), interleukin-4(IL-4), IL-2 and IL-5 were examined before and after treatments.RESULTS: The level of Th1 cytokines(INF-γ, IL-2)and the ratio of Th1/Th2 cytokines(INF-γ/IL-4, IL-2/IL-5) increased in both treatment groups, while Th2cytokines(IL-4, IL-5) and FACT-L scores decreased(P<0.01). Furthermore, after treatment Th1 cytokines(INF-γ, IL-2) and the ratio of Th1/Th2 cytokines(INF-γ/IL-4, IL-2/IL-5) were higher in the DCs +GPS group than in the control group(P<0.05). Conversely, FACT-L scores and Th2 cytokines(IL-4, IL-5)were higher in the control group than in the DCs +GPS group(P<0.05).CONCLUSION: The treatment regime of DCs plus GPS had a greater effect on NSCLC patients' immune function as compared with DCs alone. This was evident by increased expression of Th1 cytokines(INF-γ, IL-2) and the ratio of Th1/Th2(INF-γ/IL-4, IL-2/IL-5), as well as by decreased FACT-L scores and the expression of Th2 cytokines(IL-4,IL-5).展开更多
基金supported by Poten Biomedical Technology Development Co.,Ltd.
文摘<Abstract>Background and Objective:Cytokine-induced killer(CIK) cells and autologous dendritic cells-CIK(DCCIK) cells co-cultured with autologous dendritic cells(DCs) and CIK cells are commonly used for immunotherapy recently.We compared the anti-tumor immune response of CIK cells,autologous DC-CIK cells,and semi-allogeneic DC-CIK cells to explore a more effective anti-tumor adoptive immunotherapy approach.Met hods:Peripheral monocytes were isolated from patients with renal carcinoma,lung cancer,or maxillary squamous cell carcinoma and their healthy adult children.Isolated cells were cultured and induced as DCs and CIK cells in vitro.CIK cells from patients were co-cultured with autologous DCs and DCs from their children respectively,generating DC-CIK cells and semi-allogeneic DC-CIK cells.The anti-tumor activities of autologous CIK cells,autologous DC-CIK cells,and semi-allogeneic DC-CIK cells were measured by LDH assay.Intracellular staining was used to test the secretion of cytokines.Flow cytometry was applied for detecting the phonotype changes of these three types of cells.Cell proliferation and cell apoptosis were detected by 5,6carboxyfluorescein diacetate succinimidyl ester(CFSE) and Annexin V/PI respectively.Result s:Compared with autologous CIK cells and DC-CIK cells,semi-allogeneic DC-CIK cells significantly enhanced the anti-tumor activity and IFN-γ secretion,reduced IL-4 secretion,increased the ratio of CD3+CD56+ cells and CD3+CD8+ cells,decreased the number of CD4+CD25+ cells,promoted cell proliferation,and lessened cell apoptosis.Conclusions:Semi-allogeneic DC-CIK cells had a stronger anti-tumor effect than did autologous CIK cells and DC-CIK cells.Our results provided experimental evidence for clinical application of DC-CIK cells.
文摘目的通过体外试验研究黄芪多糖(Astragalus mongholicus,ASP)对树突状细胞(dendrit- ic cells,DC)功能调节的机制,为进一步阐明黄芪多糖的免疫学活性提供依据。方法应用流式细胞仪检测技术、扫描电镜技术、酶联免疫吸附试验检测DC表型和功能的各种指标。结果本实验应用小鼠骨髓来源的DC,通过体外试验证明了黄芪多糖能够提高DC表面分子CD11c和MHCⅡ的表达,并且呈黄芪多糖浓度依赖性;空白组DC的吞噬功能很强,LPS组DC和黄芪多糖处理组DC吞噬功能都明显下降;黄芪多糖能够促进DC白细胞介素-12(IL-12)的表达;电镜观察DC的超微结构,可见黄芪多糖处理组DC突起增多,形态上更加成熟。结论本实验结果证实了黄芪多糖能促进小鼠骨髓来源的DC表型及功能的成熟。
基金2011 National Key Specialty Construction of Clinical Projects of ChinaThe National Natural Science Foundation of China(No.81273639)Science and Technology Projects of Traditional Chinese Medicine Bureau in Jiangsu Province of China(No.LB13042)
文摘OBJECTIVE: To investigate the effect of thorascopic administration of ginseng polysaccharides(GPS)plus dendritic cells(DC) on T helper cell type 1/T helper cell type 2(Th1/Th2) balance in patients with non-small cell lung cancer(NSCLC).METHODS: A total of 96 NSCLC patients were divided evenly into two groups. The control group was treated with DCs alone and the treatment group was treated with DCs plus GPS. After DCs and GPS were administered thoracoscopically, once a week,4 times for 30 days, the patients' quality of life was measured with the Functional Assessment of Can-cer Treatment-Lung(FACT-L) questionnaire before and after treatment. Serum interferon-γ(INF-γ), interleukin-4(IL-4), IL-2 and IL-5 were examined before and after treatments.RESULTS: The level of Th1 cytokines(INF-γ, IL-2)and the ratio of Th1/Th2 cytokines(INF-γ/IL-4, IL-2/IL-5) increased in both treatment groups, while Th2cytokines(IL-4, IL-5) and FACT-L scores decreased(P<0.01). Furthermore, after treatment Th1 cytokines(INF-γ, IL-2) and the ratio of Th1/Th2 cytokines(INF-γ/IL-4, IL-2/IL-5) were higher in the DCs +GPS group than in the control group(P<0.05). Conversely, FACT-L scores and Th2 cytokines(IL-4, IL-5)were higher in the control group than in the DCs +GPS group(P<0.05).CONCLUSION: The treatment regime of DCs plus GPS had a greater effect on NSCLC patients' immune function as compared with DCs alone. This was evident by increased expression of Th1 cytokines(INF-γ, IL-2) and the ratio of Th1/Th2(INF-γ/IL-4, IL-2/IL-5), as well as by decreased FACT-L scores and the expression of Th2 cytokines(IL-4,IL-5).
