The adsorption and leaching of chalcopyrite by two extreme thermophilic archaea(A.brierleyi and S.metallicus)and their mixture were studied.The results revealed that the chalcopyrite leaching rate of S.metallicus was ...The adsorption and leaching of chalcopyrite by two extreme thermophilic archaea(A.brierleyi and S.metallicus)and their mixture were studied.The results revealed that the chalcopyrite leaching rate of S.metallicus was slightly higher than that of A.brierleyi;the mixed system showed the highest rate.Community structure analysis during the leaching process showed that S.metallicus was maintained in a predominant state.However,the proportion of A.brierleyi in the community increased during leaching.Copper concentrations,which increased faster in the mixed system than in the single-organism systems during later stages,was related to the change of A.brierleyi in the community.Langmuir parameter analysis revealed no competitive adsorption between these two thermophilic archaea.Furthermore,qPCR(quantitative polymerase chain reaction)confirmed that adsorption was promoted between A.brierleyi and S.metallicus during mixed leaching.These findings can improve our understanding of the adsorption behaviors of mixed extreme microbial populations on mineral surfaces.展开更多
高温会加快碱基脱氨基反应形成损伤碱基的速率,进一步对脱氨基的碱基进行复制会导致突变。因此,极端嗜热古菌基因组的稳定性面临着其生存高温环境的挑战。胞嘧啶脱氨基形成尿嘧啶,是常见的脱碱基类型,复制DNA中尿嘧啶会造成GC→AT的突...高温会加快碱基脱氨基反应形成损伤碱基的速率,进一步对脱氨基的碱基进行复制会导致突变。因此,极端嗜热古菌基因组的稳定性面临着其生存高温环境的挑战。胞嘧啶脱氨基形成尿嘧啶,是常见的脱碱基类型,复制DNA中尿嘧啶会造成GC→AT的突变。尿嘧啶DNA糖苷酶(Uracil DNA glycosylase,UDG)是修复DNA中尿嘧啶的关键酶。基于识别底物的特异性,UDG分为6个家族,广泛分布在细菌、古菌、真核生物以及一些病毒中。基因组序列显示,极端嗜热古菌至少编码一种UDG。目前,对于细菌和真核生物的UDG已进行了大量的研究,但是关于极端嗜热古菌UDG的研究相对较少,尚处于初期阶段。本文综述了极端嗜热古菌UDG的研究进展,并对今后的研究提出了展望。展开更多
The effect of L-cysteine in different concentrations on the bioleaching of Ni-Cu sulfide was studied with an extremely thermophilic archaea,Acidianus manzaensis. It is found that adding certain amounts of L-cysteine t...The effect of L-cysteine in different concentrations on the bioleaching of Ni-Cu sulfide was studied with an extremely thermophilic archaea,Acidianus manzaensis. It is found that adding certain amounts of L-cysteine to the bioleaching system of Ni-Cu sulfide largely enhances the leaching rate. X-ray diffraction (XRD) patterns show the change of bioleached solid residues and the effect of L-cysteine on the surface charges of minerals. Zeta potential and IR spectra of mineral surface show that the interaction between L-cysteine and mineral leads to the formation of metal complex,which is propitious to the bioleaching of Ni-Cu sulfide by Acidianus manzaensis.展开更多
基金Project (51774342) supported by the National Natural Science Foundation of ChinaProject (2016RS2016) supproted by the Hunan Provincial Science and Technology Leader (Innovation Team of Interface Chemistry of Efficient and Clean Utilization of Complex Mineral Resources),China
文摘The adsorption and leaching of chalcopyrite by two extreme thermophilic archaea(A.brierleyi and S.metallicus)and their mixture were studied.The results revealed that the chalcopyrite leaching rate of S.metallicus was slightly higher than that of A.brierleyi;the mixed system showed the highest rate.Community structure analysis during the leaching process showed that S.metallicus was maintained in a predominant state.However,the proportion of A.brierleyi in the community increased during leaching.Copper concentrations,which increased faster in the mixed system than in the single-organism systems during later stages,was related to the change of A.brierleyi in the community.Langmuir parameter analysis revealed no competitive adsorption between these two thermophilic archaea.Furthermore,qPCR(quantitative polymerase chain reaction)confirmed that adsorption was promoted between A.brierleyi and S.metallicus during mixed leaching.These findings can improve our understanding of the adsorption behaviors of mixed extreme microbial populations on mineral surfaces.
文摘高温会加快碱基脱氨基反应形成损伤碱基的速率,进一步对脱氨基的碱基进行复制会导致突变。因此,极端嗜热古菌基因组的稳定性面临着其生存高温环境的挑战。胞嘧啶脱氨基形成尿嘧啶,是常见的脱碱基类型,复制DNA中尿嘧啶会造成GC→AT的突变。尿嘧啶DNA糖苷酶(Uracil DNA glycosylase,UDG)是修复DNA中尿嘧啶的关键酶。基于识别底物的特异性,UDG分为6个家族,广泛分布在细菌、古菌、真核生物以及一些病毒中。基因组序列显示,极端嗜热古菌至少编码一种UDG。目前,对于细菌和真核生物的UDG已进行了大量的研究,但是关于极端嗜热古菌UDG的研究相对较少,尚处于初期阶段。本文综述了极端嗜热古菌UDG的研究进展,并对今后的研究提出了展望。
基金Projects(50621063, 30400010) supported by the National Natural Science Foundation of ChinaProject(2010CB630903) supported by the National Basic Research Program of China
文摘The effect of L-cysteine in different concentrations on the bioleaching of Ni-Cu sulfide was studied with an extremely thermophilic archaea,Acidianus manzaensis. It is found that adding certain amounts of L-cysteine to the bioleaching system of Ni-Cu sulfide largely enhances the leaching rate. X-ray diffraction (XRD) patterns show the change of bioleached solid residues and the effect of L-cysteine on the surface charges of minerals. Zeta potential and IR spectra of mineral surface show that the interaction between L-cysteine and mineral leads to the formation of metal complex,which is propitious to the bioleaching of Ni-Cu sulfide by Acidianus manzaensis.
