摘要
7,8二氢-8-氧鸟嘌呤(7,8-dihydro-8-oxoguanine,8oxoG)是一种常见的DNA损伤碱基。由于8oxoG能够与腺嘌呤配对,在DNA中的8oxoG被修复之前进行复制,DNA将会产生GC→TA的突变,从而造成基因组的不稳定。目前,碱基切除修复(Base excision repair,BER)是修复DNA中8oxoG的经典途径,其中8oxoGDNA糖苷酶(8-oxoguanineDNAglycosylases,OGG)是启动BER途径的关键酶。研究发现,OGG能够识别和切除DNA中的8oxoG,从而阻止细胞内GC→TA突变的积累。目前,OGG分为3个家族:OGG1、OGG2和AGOG (archaeal 8oxoG DNA glycosylase),广泛分布于细菌、古菌和真核生物。古菌基因组的序列表明,它们至少编码一种OGG。目前,对源自细菌和真核生物的OGG已进行了大量的研究,但是关于极端嗜热古菌OGG的研究相对较少,尚处于初期阶段。本文综述了极端嗜热古菌OGG的研究进展,并对今后的研究提出了展望。
7,8-dihydro-8-oxoguanine(7,8-dihydro-8-oxoguanine,8oxoG)is a common DNA damage base.Because 8oxoG can form a pair with adenine,the replication of 8oxoG before being repaired would lead to the mutation of GC→TA,thereby causing genome instability.Base excision repair(BER)is a typical pathway to repair 8oxoG in DNA,among which 8oxoG DNA glycosylases(OGGs)are the key enzymes that initiate a BER pathway.Previous studies showed that OGG can recognize and excise 8oxoG in DNA,thereby preventing the accumulation of GC→TA mutations in cells.Currently,OGG,which is widely distributed in bacteria,archaea and eukaryotes,has been divided into three families:OGG1,OGG2 and AGOG(archaeal 8oxoG DNA glycosylase).Archaeal genomic sequences suggest that they encode at least one OGG.Currently,a few OGGs of bacteria and eukaryotes have been extensively studied,but there have been several studies on OGGs of hyperthermophilic archaea,which is still in the early stage.Research progress of OGGs of hyperthermophilic archaea was reviewed and the prospects for future research were proposed in this article.
作者
吴颖
陈鸿迅
张立奎
Ying Wu;Hongxun Chen;Likui Zhang(Marine Science&Technology Institute,Department of Environmental Science and Engineering,Yangzhou University,Yangzhou 225127,Jiangsu Province,China;College of Guangling,Yangzhou University,Yangzhou 225128,Jiangsu Province,China)
出处
《微生物学报》
CAS
CSCD
北大核心
2021年第3期491-500,共10页
Acta Microbiologica Sinica
基金
江苏省自然科学基金(BK20191219)
扬州大学中青年学术带头人项目
扬州大学大学生科技创新项目(X20190523)。
