Prirnula interjacens Chen (Primulaceae) is a rare and narrow endemic species of centralsouth of Yunnan Province in China. This species consists of two varieties: P.interjacens var. interjacens known with only one popu...Prirnula interjacens Chen (Primulaceae) is a rare and narrow endemic species of centralsouth of Yunnan Province in China. This species consists of two varieties: P.interjacens var. interjacens known with only one population, and P.interjacens var. epilosa with two populations. Intersimple sequence repeat (ISSR) marker was used to detect the genetic diversity of the three extant populations. We expected a low genetic diversity level, but our results revealed a high level of intraspecific genetic diversity (at population level: P=59.75%, HE=0.2368, and Hpop=0.3459; at species level: P=75.47%, HT= 0.320 5, and Hsp = 0.4618), probably resulting from floral heteromorphism and preferring outcrossing. A moderate level of genetic differentiation among populations was detected based on Nei's genetic diversity analysis (26.13%) and Shannon's diversity index (25.09%). Although P./ntedacens var. intedacens and P. interjacens var. epilosa were morphologically distinct, UPGMA cluster analysis showed that the two varieties had no distinct genetic differentiation and may be treated as a single taxon. Conservation measures are suggested, including in situ and ex situ strategies, based on the observed population genetic information.展开更多
用报春花叶片、花蕾作外植体,接种在含有不同浓度6BA、NAA 的 MS 培养基上,诱导愈伤组织形成,经愈伤组织增殖和反复切割后诱导其分化出芽和根,然后将试管苗移植到土壤中。报春花(Primula malacoides),是报春花科报春花属的二年生草本花...用报春花叶片、花蕾作外植体,接种在含有不同浓度6BA、NAA 的 MS 培养基上,诱导愈伤组织形成,经愈伤组织增殖和反复切割后诱导其分化出芽和根,然后将试管苗移植到土壤中。报春花(Primula malacoides),是报春花科报春花属的二年生草本花卉。产于云南、广西西部,其花期长,观赏价值高。多年来均用种子繁殖,速度较慢。如改用组织培养法,可大大提高其生产效率。但其组织培养工作在国内外尚未见报道,为此,我们对其进行了愈伤组织的诱导和植株再生的研究,得到了初步结果。展开更多
文摘Prirnula interjacens Chen (Primulaceae) is a rare and narrow endemic species of centralsouth of Yunnan Province in China. This species consists of two varieties: P.interjacens var. interjacens known with only one population, and P.interjacens var. epilosa with two populations. Intersimple sequence repeat (ISSR) marker was used to detect the genetic diversity of the three extant populations. We expected a low genetic diversity level, but our results revealed a high level of intraspecific genetic diversity (at population level: P=59.75%, HE=0.2368, and Hpop=0.3459; at species level: P=75.47%, HT= 0.320 5, and Hsp = 0.4618), probably resulting from floral heteromorphism and preferring outcrossing. A moderate level of genetic differentiation among populations was detected based on Nei's genetic diversity analysis (26.13%) and Shannon's diversity index (25.09%). Although P./ntedacens var. intedacens and P. interjacens var. epilosa were morphologically distinct, UPGMA cluster analysis showed that the two varieties had no distinct genetic differentiation and may be treated as a single taxon. Conservation measures are suggested, including in situ and ex situ strategies, based on the observed population genetic information.
文摘用报春花叶片、花蕾作外植体,接种在含有不同浓度6BA、NAA 的 MS 培养基上,诱导愈伤组织形成,经愈伤组织增殖和反复切割后诱导其分化出芽和根,然后将试管苗移植到土壤中。报春花(Primula malacoides),是报春花科报春花属的二年生草本花卉。产于云南、广西西部,其花期长,观赏价值高。多年来均用种子繁殖,速度较慢。如改用组织培养法,可大大提高其生产效率。但其组织培养工作在国内外尚未见报道,为此,我们对其进行了愈伤组织的诱导和植株再生的研究,得到了初步结果。
