摘要
用报春花叶片、花蕾作外植体,接种在含有不同浓度6BA、NAA 的 MS 培养基上,诱导愈伤组织形成,经愈伤组织增殖和反复切割后诱导其分化出芽和根,然后将试管苗移植到土壤中。报春花(Primula malacoides),是报春花科报春花属的二年生草本花卉。产于云南、广西西部,其花期长,观赏价值高。多年来均用种子繁殖,速度较慢。如改用组织培养法,可大大提高其生产效率。但其组织培养工作在国内外尚未见报道,为此,我们对其进行了愈伤组织的诱导和植株再生的研究,得到了初步结果。
Leaf blade and flower bud of Primula malaioides was cultured on MS Supplemented with different concentration of 6—BA and NAA.Callus was derived from explant,after callus has been rapidly was transfered to medium that induce Shoot and root formation,The test—tube plantlet was transplanted into soil.
出处
《东北师大学报（自然科学版）》
CAS
CSCD
1992年第4期94-97,共4页
Journal of Northeast Normal University(Natural Science Edition)
关键词
报春花
组织培养
植株再生
primala malacoides
tissure culture, plantl regeneration
callus
test-tube Plantlet.
