目的为了获得能够与人67ku层黏连蛋白受体(67 ku laminin receptor,67LR)特异性结合的噬菌体呈现肽。方法以重组人67LR蛋白为诱饵,分别对噬菌体12肽库和环7肽库进行亲和凝胶筛选,ELISA测定所筛选噬菌体克隆与靶蛋白的亲和力,经测序分析...目的为了获得能够与人67ku层黏连蛋白受体(67 ku laminin receptor,67LR)特异性结合的噬菌体呈现肽。方法以重组人67LR蛋白为诱饵,分别对噬菌体12肽库和环7肽库进行亲和凝胶筛选,ELISA测定所筛选噬菌体克隆与靶蛋白的亲和力,经测序分析得到67LR特异性结合噬菌体克隆,并通过293细胞侵袭转移实验测定阳性噬菌体呈现肽对67LR促细胞侵袭作用的抑制效果。结果经4轮筛选共筛到2类新的67LR结合肽,分别具有DXCETCT(X可变)和YRPMXEY(X可变)一致序列。其中DX-CETCT噬菌体呈现肽可显著抑制67LR所诱导的细胞侵袭,且这种抑制作用与YIGSR5肽具有一定互补性。结论成功筛选到2类新的67LR结合肽,其中DXCETCT噬菌体呈现肽可针对性抑制67LR所诱导的细胞侵袭作用。展开更多
AIM To investigate the expressions and singnificance of laminin and laminin receptors in human cervical carcinoma. METHODS Tissues from normal cervices, 26 cases with carcinoma in situ , 21 cases with microinvasive ca...AIM To investigate the expressions and singnificance of laminin and laminin receptors in human cervical carcinoma. METHODS Tissues from normal cervices, 26 cases with carcinoma in situ , 21 cases with microinvasive cancer and 18 cases with frankly invasive cancer were observed by SABC immunohistochemical method for expressions of laminin and laminin receptors. RESULTS The positive staining for laminin was observed in normalcervical tissues and carcinoma in situ , but was weak or abscent in cervical invasive carcinoma tissues.( P <0.05). The staining for laminin receptors of normal cervical tissues and carcinoma in situ was negative, but was positive in cervical invasive carcinoma tissues ( P <0.05). CONCLUSION There were positive changes in laminin and laminin receptors in cervical invasive carcinoma tissues. These changes may be very useful in the pathologic diagnosis and prognosis of human cervical carcinomas.展开更多
文摘目的为了获得能够与人67ku层黏连蛋白受体(67 ku laminin receptor,67LR)特异性结合的噬菌体呈现肽。方法以重组人67LR蛋白为诱饵,分别对噬菌体12肽库和环7肽库进行亲和凝胶筛选,ELISA测定所筛选噬菌体克隆与靶蛋白的亲和力,经测序分析得到67LR特异性结合噬菌体克隆,并通过293细胞侵袭转移实验测定阳性噬菌体呈现肽对67LR促细胞侵袭作用的抑制效果。结果经4轮筛选共筛到2类新的67LR结合肽,分别具有DXCETCT(X可变)和YRPMXEY(X可变)一致序列。其中DX-CETCT噬菌体呈现肽可显著抑制67LR所诱导的细胞侵袭,且这种抑制作用与YIGSR5肽具有一定互补性。结论成功筛选到2类新的67LR结合肽,其中DXCETCT噬菌体呈现肽可针对性抑制67LR所诱导的细胞侵袭作用。
文摘AIM To investigate the expressions and singnificance of laminin and laminin receptors in human cervical carcinoma. METHODS Tissues from normal cervices, 26 cases with carcinoma in situ , 21 cases with microinvasive cancer and 18 cases with frankly invasive cancer were observed by SABC immunohistochemical method for expressions of laminin and laminin receptors. RESULTS The positive staining for laminin was observed in normalcervical tissues and carcinoma in situ , but was weak or abscent in cervical invasive carcinoma tissues.( P <0.05). The staining for laminin receptors of normal cervical tissues and carcinoma in situ was negative, but was positive in cervical invasive carcinoma tissues ( P <0.05). CONCLUSION There were positive changes in laminin and laminin receptors in cervical invasive carcinoma tissues. These changes may be very useful in the pathologic diagnosis and prognosis of human cervical carcinomas.
