Fragments of nuclear ribosomal 28S rDNA D2 divergent domain, mitochondrial 16S rDNA, and COI partial genes of 15 species in the subgenus Singhardina Mahmood from China were amplified and sequenced. Molecular phylogene...Fragments of nuclear ribosomal 28S rDNA D2 divergent domain, mitochondrial 16S rDNA, and COI partial genes of 15 species in the subgenus Singhardina Mahmood from China were amplified and sequenced. Molecular phylogenetic trees were constructed using maximum parsimony, maximum likelihood and Bayesian inference methods. Results from these methods revealed similar topologies with recognizable relationships among subclades. The phylogenetic relationship of four species groups of subgenus Singhardina Mahmood from China is discussed for the first time. The results show that Singhardina Mahmood forms a single lineage representing a monophyletic group. The Eurhadina （Singhardina） rubra species group newly proposed in this study is likely the most basal species group within Singhardina Mahmood. The E. （Singhardina） robusta species group is the sister group of the E. （Singhardina） mamata species group. Molecular evidence supports including the E. （Singhardina） vittata species group in the E. （Singhardina） punjabensis species group.展开更多
基金supported by the Key Technologies R&D Program of Guiyang City (No.[2009]3-0243)Guizhou Provincial Natural Science Foundation of China (No.[2010]2063)
基金supported by"The National Natural Science Foundation of China" (No. 30770262)"The Ministry of Science and Technology of the People's Republic of China " (No. 2006FY120100)partly by "Northwest A&F University Grants for Outstanding Young Faculty Members" (No.Z111020905)
文摘Fragments of nuclear ribosomal 28S rDNA D2 divergent domain, mitochondrial 16S rDNA, and COI partial genes of 15 species in the subgenus Singhardina Mahmood from China were amplified and sequenced. Molecular phylogenetic trees were constructed using maximum parsimony, maximum likelihood and Bayesian inference methods. Results from these methods revealed similar topologies with recognizable relationships among subclades. The phylogenetic relationship of four species groups of subgenus Singhardina Mahmood from China is discussed for the first time. The results show that Singhardina Mahmood forms a single lineage representing a monophyletic group. The Eurhadina （Singhardina） rubra species group newly proposed in this study is likely the most basal species group within Singhardina Mahmood. The E. （Singhardina） robusta species group is the sister group of the E. （Singhardina） mamata species group. Molecular evidence supports including the E. （Singhardina） vittata species group in the E. （Singhardina） punjabensis species group.
