Objective: To investigate whether bacterial DNA involving in the pathogenesis of systemic inflammatory response syndrome (SIRS) and possible mechanism. Methods: Escherichia coli DNA (EC DNA) was extracted from Escheri...Objective: To investigate whether bacterial DNA involving in the pathogenesis of systemic inflammatory response syndrome (SIRS) and possible mechanism. Methods: Escherichia coli DNA (EC DNA) was extracted from Escherichia coli 25922 with alkaline lysis method. The mice mortality was observed after EC DNA was injected into mice via caudal vein. The changes of serum TNF-α and IL-6 levels in rats were measured with ELISA after rats were given EC DNA. Calf thymus DNA and lipopolysaccharide (LPS) were used as the control, respectively. Results: EC DNA led mice to death with notable dose-effect relationship (LD50=11.51 mg/kg), but CT DNA didn't. The peak level of TNF-αwas lower in EC DNA group than in LPS group (P＜0. 05), though the former reaching the peak I h earlier than the latter. However, they had coordinate ability to induce IL-6 release in rats, and no significant difference was seen in serum IL-6 peak level between 2 groups. Conclusion: EC DNA leads mice to death, and induces the increases of serum TNF-αand IL-6 levels in rats. EC DNA has the effect equal to LPS in inducing SIRS by triggering cytokines cascade.展开更多
据美国BIOCOMPARE科技新闻网（2008／4／8）报道，美国密执安州立大学（Michigan State University）的研究人员，开发了一项新的技术能够快速的侦测大肠杆菌DNA的变异性，他们观察了500多种不同的大肠杆菌科的致病菌，并观察DNA中的单...据美国BIOCOMPARE科技新闻网（2008／4／8）报道，美国密执安州立大学（Michigan State University）的研究人员，开发了一项新的技术能够快速的侦测大肠杆菌DNA的变异性，他们观察了500多种不同的大肠杆菌科的致病菌，并观察DNA中的单点核酸变异（SNPs），分析出96个指标，希望在疾病未爆发前就能找到正确的病源，此研究发表于2008年3月10日的PNAS在线报道，由Thomas Whittam博士主导研究进行。展开更多
基金National Natural Science Foundation of China (No. 30070299)
文摘Objective: To investigate whether bacterial DNA involving in the pathogenesis of systemic inflammatory response syndrome (SIRS) and possible mechanism. Methods: Escherichia coli DNA (EC DNA) was extracted from Escherichia coli 25922 with alkaline lysis method. The mice mortality was observed after EC DNA was injected into mice via caudal vein. The changes of serum TNF-α and IL-6 levels in rats were measured with ELISA after rats were given EC DNA. Calf thymus DNA and lipopolysaccharide (LPS) were used as the control, respectively. Results: EC DNA led mice to death with notable dose-effect relationship (LD50=11.51 mg/kg), but CT DNA didn't. The peak level of TNF-αwas lower in EC DNA group than in LPS group (P＜0. 05), though the former reaching the peak I h earlier than the latter. However, they had coordinate ability to induce IL-6 release in rats, and no significant difference was seen in serum IL-6 peak level between 2 groups. Conclusion: EC DNA leads mice to death, and induces the increases of serum TNF-αand IL-6 levels in rats. EC DNA has the effect equal to LPS in inducing SIRS by triggering cytokines cascade.
文摘据美国BIOCOMPARE科技新闻网（2008／4／8）报道，美国密执安州立大学（Michigan State University）的研究人员，开发了一项新的技术能够快速的侦测大肠杆菌DNA的变异性，他们观察了500多种不同的大肠杆菌科的致病菌，并观察DNA中的单点核酸变异（SNPs），分析出96个指标，希望在疾病未爆发前就能找到正确的病源，此研究发表于2008年3月10日的PNAS在线报道，由Thomas Whittam博士主导研究进行。
