摘要
目的 :提取双歧杆菌、大肠杆菌DNA ,与iIEL细胞共同孵育 ,观察它们对IEL细胞的激活作用 ,并用DNA免疫小鼠 ,观察小鼠免疫功能的变化 ,探讨双歧杆菌、大肠杆菌DNA对免疫功能的影响 ,并作对比研究。方法 :以自然杀伤细胞 (NK)活性 ,白细胞介素 2 (IL 2 )产生能力为指标 ,测定小鼠上述各项指标变化。结果 :小鼠以上两项指标与对照组相比有明显提高(P <0 .0 5 )。双歧杆菌DNA提高小鼠NK活性与IL 2水平程度大于大肠杆菌DNA的作用 (P <0 .0 1)。结论 :表明双歧杆菌DNA可快速激活NK活性 ,提高体内IL 2水平。
Objective:To extract DNA from Bifidobacteria and E.coli cells and incubate DNA with IEL to observe.DNA's activation for IEL.Also plan to use the DNA to immune mice to observe the change of immunologic function,discuss the effect of Bifidobacteria and E.coli's whole DNA to murine and make a comparision study.Methods: Mice of Bifidobacteria DNA and E.coli's DNA treated groups are respectively given with DNA by muscular and subcutaneous injection. Detect the immunologic function of spleen cell, extract IEL cells and incubate them with DNA, detecting IEL activity and the production of cytokine. Results:The activities of IL 2 and NK are all significantly higer than those of in control groups (P<0.01). IL 2 and NK's activities of Bifidobacteria DNA group are higher than those of E.coli DNA groups (P<0.01). Conclusion: The Bifidobacteria DNA and E.coli DNA can regulate mocosal immunity by enlarging natural killing activity and secreting cytokine as IL 2. The Bifidobacteria DNA is better in activation IEL than E.coli DNA.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2001年第7期356-357,共2页
Chinese Journal of Immunology
基金
辽宁省自然科学基金资助 (962 3 16)
