Objective：To establish the polytransgenic mice expressing the human HT and complement regulatory proteins （CRPs） and discuss their ability to resist the hyperacute rejection （HAR） and delayed xenograft rejection ...Objective：To establish the polytransgenic mice expressing the human HT and complement regulatory proteins （CRPs） and discuss their ability to resist the hyperacute rejection （HAR） and delayed xenograft rejection （DXR） of heterogenic transplantation. Methods ：Transgenic mice were produced by microinjection to construct gene for human HT, delay acceleration factor （DAF） and/or CD59 into the male pronucleus of zygote. PCR and Southern blot were used to screen the positive trarisgenic mice. Flow cytometry （FCM） was used to detect the expression of HT, ct-Gal and DAF or CD59 on the PBMCs of transgenic mice. The survival time and function of the heart of transgenic mice were determined by a modified Langendorff cardiac perfusion apparatus： The change of proteinosis on IgM,IgG, C3c and C9 from different cardiac vascular iendothelial cells of transgenic mice were detected by immunohistochemistry. Results：HT, DAF or CD59 were highly expressed on the positive transgenic mice by FCM. The deposition of IgM,IgG,C3c or C9 in the cardiac vascular endothelial cells of the positive transgenic mice were de- creased. The survival time and function of the heart of the co-transgenic mice with AB serum perfusion were significantly longer and higher than that of the single HT positive transgenic mice（P 〈0.05）. Conclusion ：The mice co-expressing HT/DAF or HT/CD59 could resist the HAR,which was better than those expressing HT alone. It is feasible to use HT and CRPs co-transgenic methods to resist the HAR and DXR.展开更多
调谐黏滞质量阻尼器(Tuned Viscous Mass Damper,TVMD)是一种有效的被动惯容减震装置,本文针对地震作用下建筑结构TVMD阻尼比增效效应与优化设计展开研究。将TVMD对结构自身阻尼耗能功率的控制效果归纳为TVMD等效附加阻尼比,并基于随机...调谐黏滞质量阻尼器(Tuned Viscous Mass Damper,TVMD)是一种有效的被动惯容减震装置,本文针对地震作用下建筑结构TVMD阻尼比增效效应与优化设计展开研究。将TVMD对结构自身阻尼耗能功率的控制效果归纳为TVMD等效附加阻尼比,并基于随机振动理论推导了等效附加阻尼比的理论表达式。为了使TVMD更具实际应用价值,TVMD理论上应取得比同阻尼系数的黏滞阻尼器(VD)更大的等效附加阻尼比,这一现象定义为TVMD阻尼比增效效应,并定义了阻尼比增效系数来量化评估阻尼比增效效应。将等效附加阻尼比和阻尼比增效系数均作为优化目标,提出了TVMD最优设计参数理论解。参数分析结果表明,本文解具有良好的稳定性和适用性,为了更高效地发挥阻尼比增效效应,推荐TVMD质量比不超过0.3或阻尼比不超过0.1。以某七层标准钢框架结构作为工程算例展示了TVMD设计流程,并验证了本文解的有效性和优越性。算例分析结果表明,使用本文解设计TVMD能显著放大其阻尼元件变形,表现出了理想的阻尼比增效效应。与传统解相比,本文解还具有另一个明显优势,即保证TVMD的减震效果优于同阻尼系数的VD,不存在减震效率问题。展开更多
基金Tianjin Municipal Science and Technology CommissionGrant number:043803411
文摘Objective：To establish the polytransgenic mice expressing the human HT and complement regulatory proteins （CRPs） and discuss their ability to resist the hyperacute rejection （HAR） and delayed xenograft rejection （DXR） of heterogenic transplantation. Methods ：Transgenic mice were produced by microinjection to construct gene for human HT, delay acceleration factor （DAF） and/or CD59 into the male pronucleus of zygote. PCR and Southern blot were used to screen the positive trarisgenic mice. Flow cytometry （FCM） was used to detect the expression of HT, ct-Gal and DAF or CD59 on the PBMCs of transgenic mice. The survival time and function of the heart of transgenic mice were determined by a modified Langendorff cardiac perfusion apparatus： The change of proteinosis on IgM,IgG, C3c and C9 from different cardiac vascular iendothelial cells of transgenic mice were detected by immunohistochemistry. Results：HT, DAF or CD59 were highly expressed on the positive transgenic mice by FCM. The deposition of IgM,IgG,C3c or C9 in the cardiac vascular endothelial cells of the positive transgenic mice were de- creased. The survival time and function of the heart of the co-transgenic mice with AB serum perfusion were significantly longer and higher than that of the single HT positive transgenic mice（P 〈0.05）. Conclusion ：The mice co-expressing HT/DAF or HT/CD59 could resist the HAR,which was better than those expressing HT alone. It is feasible to use HT and CRPs co-transgenic methods to resist the HAR and DXR.
文摘调谐黏滞质量阻尼器(Tuned Viscous Mass Damper,TVMD)是一种有效的被动惯容减震装置,本文针对地震作用下建筑结构TVMD阻尼比增效效应与优化设计展开研究。将TVMD对结构自身阻尼耗能功率的控制效果归纳为TVMD等效附加阻尼比,并基于随机振动理论推导了等效附加阻尼比的理论表达式。为了使TVMD更具实际应用价值,TVMD理论上应取得比同阻尼系数的黏滞阻尼器(VD)更大的等效附加阻尼比,这一现象定义为TVMD阻尼比增效效应,并定义了阻尼比增效系数来量化评估阻尼比增效效应。将等效附加阻尼比和阻尼比增效系数均作为优化目标,提出了TVMD最优设计参数理论解。参数分析结果表明,本文解具有良好的稳定性和适用性,为了更高效地发挥阻尼比增效效应,推荐TVMD质量比不超过0.3或阻尼比不超过0.1。以某七层标准钢框架结构作为工程算例展示了TVMD设计流程,并验证了本文解的有效性和优越性。算例分析结果表明,使用本文解设计TVMD能显著放大其阻尼元件变形,表现出了理想的阻尼比增效效应。与传统解相比,本文解还具有另一个明显优势,即保证TVMD的减震效果优于同阻尼系数的VD,不存在减震效率问题。
