摘要
目的探讨扇贝多肽对环磷酰胺(CTX)抗肿瘤作用的增效作用。方法无菌抽取荷H22小鼠腹水,于小鼠右前肢腋部皮下接种瘤液0.2mL/只。小鼠随机分为对照组、环磷酰胺组(CTX 10 mg.kg-1)、扇贝多肽大、中、小剂量组(1000,500和250 mg.kg-1)和扇贝多肽大、中剂量分别与CTX联用药组共7组。扇贝多肽连续灌胃给药10d,CTX连续腹腔注射10d,对照组给于同体积的生理盐水。末次给药24h后处死动物,解剖称体重、瘤重,计算抑瘤率,比较单用扇贝多肽与联合用药的药效差别。结果实验表明,单用扇贝多肽大、中、小剂量对H22均无明显抑瘤作用。在同样剂量下分别与CTX联用后,扇贝多肽大、中剂量组抑瘤率分别为55.9%和52.9%,优于单用CTX组的抑瘤效果,说明扇贝多肽与CTX联合应用对肿瘤的抑制有较好的协同作用。结论扇贝多肽能增强CTX的抗肿瘤作用。
Objective To study the synergistic antitumor effect of PCF when combine used with CTX. Methods Inoculated 0.2 mL H22 tumor fluid into the right armpit of mouse,and then the animals were divided into seven groups randomly： control group; CTX group （CTX 10 mg. kg-1）; three test group （PCF 1000, 500 and 250 mg · kg^-1） and two combined use groups （PCF 1000, 500 mg · kg^-1with CTX 10 mg · kg^-1） . PCF were given by intragastric administration for 10 days, and CTX by intraperitoneal injection for 10 days. Same volume of saline was given to the control group. The mice were killed 24 hours after the last medication and the tumor inhibition rate was calculated. Results Three PCF groups had no effect on tumor inhibition, while two combined treatusement groups showed significant effect on tumor inhibition, while at the same doses the tumor inhibition rates were raised to 55.9% and 52.9% respectively, which were higher than that of CTX when used alone. Conclusion PCF can enhance the antitumor effects of CTX.
出处
《中国海洋药物》
CAS
CSCD
2007年第5期38-40,共3页
Chinese Journal of Marine Drugs
基金
青岛市科技局资助项目(04-HH-11-69)
关键词
扇贝多肽
环磷酰胺
抗肿瘤
增效效应
polypeptides from Chlamysfarreri（PCF）
cytoxan （CTX）
antitumor
synergism
