目的：观察胃癌患者围手术期前后及不同病理分期的外周血 T 细胞亚群及 NK 细胞和血清可溶性白细胞介素2受体（sIL-2R）浓度变化，了解胃癌患者围手术期免疫功能状况及其变化。方法连续收集我院普外科2009年5月至2011年5月胃癌手术患者...目的：观察胃癌患者围手术期前后及不同病理分期的外周血 T 细胞亚群及 NK 细胞和血清可溶性白细胞介素2受体（sIL-2R）浓度变化，了解胃癌患者围手术期免疫功能状况及其变化。方法连续收集我院普外科2009年5月至2011年5月胃癌手术患者135例作为治疗组，同期于我院行健康体检者50名作为对照组。通过流式细胞术检测治疗组患者术前1 e，术后7、14 e 患者 CD3＋、CD4＋、CD8＋T 细胞数，CD4＋/ CD8＋比例及 NK 细胞比率，用 ELISA 法测定血清 sIL-2R 浓度并进行比较。同期观察健康体检者上述指标，与治疗组术前1 e 指标进行比较。结果与对照组比较，胃癌患者手术前 NK 细胞比率、CD3＋ T 细胞及 CD4＋/ CD8＋比 值 均 明 显 下 降[ NK 细胞比率：（10.11±3.64）％与（28.39±5.81）％，t =-5.9，P 〈0.01；CD3＋ T 细胞阳性率：（55.60±9.61）％与（68.65±7.39）％，t =8.6，P〈0.01；CD4＋ T 细胞阳性率：（30.22±6.17）％与（47.87±4.85）％，t =8.2，P 〈0.01；CD4＋/ CD8＋比值：1.14±0.35与1.82±0.24，t =12.7，P 〈0.01]，CD8＋ T 细胞阳性率升高[（34.26±6.23）％与（27.05±7.86）％，t =-6.5，P 〈0.01]。术后14 e，患者细胞免疫功能逐渐恢复，上述指标改善与手术前相比差异均有统计学意义（P 〈0.05）。胃癌 TNM 病理分期越晚各指标改变越大，与Ⅳ期相比，Ⅰ、Ⅱ期各项指标差异均有统计学意义（P 〈0.05）；Ⅲ期差异无统计学意义（P 〉0.05）。胃癌患者术前血清 sIL-2R 浓度高于健康查体者[（575.71±34.77）U/ L 与（428.26±21.77）U/ L，t =-7.9，P 〈0.01]，且Ⅲ、Ⅳ期患者血清 sIL-2R 浓度较Ⅰ、Ⅱ期患者低（P 〈0.05）。结论胃癌患者细胞免疫功能与肿瘤负荷大小及不同病理分期相关。NK 细胞比率和 T 淋巴细胞亚群的测定、血清 sIL-2R 水平可作为胃癌围术期预后评估的监测指标。展开更多
Objective To study the roles Of VEGF,No and lymphcyte slL-2R in theonsert of ovarian hyper-stimulation syndromeOHSS) with combined determination of these factors in the liquor folliculi and plasma of OHSS patients.Met...Objective To study the roles Of VEGF,No and lymphcyte slL-2R in theonsert of ovarian hyper-stimulation syndromeOHSS) with combined determination of these factors in the liquor folliculi and plasma of OHSS patients.Methods The liquoufolliculi,blood sample of the day Of ovum-takingS1),and blood sample of the seventh dayafter ovum-takingS7) of 12 cases of moderate to severe OHSS patientsOHSS group) and 26 cases of patients without OHSScontrol group)were collected in the period of ⅣF—ET And the levels of VEGF,NO and lymphcyte slL-2R were determined to observe their regular rule of change.Results The levels Of VEGFand lymphcyte slL-2R in the liquor follichli and S1 of the OHSS group were not significantly different from those of the control groupP>0.05),but the level of NO was significantly different that of the control,groupP>0.01).The levels Of VEGF,NO and lymphcyte sIL-2R in S7 were significantly different from those of the control groupP<0.01).Conclusion VEGF may take part in the onset of OHSS.The increase of the expression of lymphcyte sIL-2R indicates the participation of immune factors in the process of onset.As excessive NO in OHSS patients exerts pathologic action,its cytotoxic effect stimulates immunocytes to realease inflammatory cell factor,which further increase the expression of VEGF and worsens the patient’s,condition.It is simple to monitor the level of NO and lymphcyte sIL-2R in blood samples.展开更多
文摘Objective To study the roles Of VEGF,No and lymphcyte slL-2R in theonsert of ovarian hyper-stimulation syndromeOHSS) with combined determination of these factors in the liquor folliculi and plasma of OHSS patients.Methods The liquoufolliculi,blood sample of the day Of ovum-takingS1),and blood sample of the seventh dayafter ovum-takingS7) of 12 cases of moderate to severe OHSS patientsOHSS group) and 26 cases of patients without OHSScontrol group)were collected in the period of ⅣF—ET And the levels of VEGF,NO and lymphcyte slL-2R were determined to observe their regular rule of change.Results The levels Of VEGFand lymphcyte slL-2R in the liquor follichli and S1 of the OHSS group were not significantly different from those of the control groupP>0.05),but the level of NO was significantly different that of the control,groupP>0.01).The levels Of VEGF,NO and lymphcyte sIL-2R in S7 were significantly different from those of the control groupP<0.01).Conclusion VEGF may take part in the onset of OHSS.The increase of the expression of lymphcyte sIL-2R indicates the participation of immune factors in the process of onset.As excessive NO in OHSS patients exerts pathologic action,its cytotoxic effect stimulates immunocytes to realease inflammatory cell factor,which further increase the expression of VEGF and worsens the patient’s,condition.It is simple to monitor the level of NO and lymphcyte sIL-2R in blood samples.
