Arabis mosaic virus(ArMV)had been found in the Gladiolus hybridus imported from the Netherlands by DAS-ELISA.Two pairs of primers were designed according to the conserved region of the coat protein(CP) gene sequence o...Arabis mosaic virus(ArMV)had been found in the Gladiolus hybridus imported from the Netherlands by DAS-ELISA.Two pairs of primers were designed according to the conserved region of the coat protein(CP) gene sequence of Arabis mosaic virus(ArMV),and two amplified bands with expected sizes of 750 bp and 250 bp were obtained by reverse transcription polymerase chain reaction(RT-PCR).The sensitivity of nested PCR was higher than that of single RT-PCR.Analysis of the partial sequenced CP gene showed that the isolate was closed to ArMV with similarity of 99.0%-100%.All the results demonstrated that the isolated virus was ArMV.展开更多
文摘Arabis mosaic virus(ArMV)had been found in the Gladiolus hybridus imported from the Netherlands by DAS-ELISA.Two pairs of primers were designed according to the conserved region of the coat protein(CP) gene sequence of Arabis mosaic virus(ArMV),and two amplified bands with expected sizes of 750 bp and 250 bp were obtained by reverse transcription polymerase chain reaction(RT-PCR).The sensitivity of nested PCR was higher than that of single RT-PCR.Analysis of the partial sequenced CP gene showed that the isolate was closed to ArMV with similarity of 99.0%-100%.All the results demonstrated that the isolated virus was ArMV.
