目的:观察慢性镉中毒对小鼠海马CA3区神经胶质细胞的影响。方法:将40只小鼠随机分为对照组与镉中毒组,每组20只。饲养1周后,镉中毒组采用皮下注射氯化镉,每周2次,连续3个月,对照组同时注射等体积生理盐水。连续喂养3个月后取小鼠海马分...目的:观察慢性镉中毒对小鼠海马CA3区神经胶质细胞的影响。方法:将40只小鼠随机分为对照组与镉中毒组,每组20只。饲养1周后,镉中毒组采用皮下注射氯化镉,每周2次,连续3个月,对照组同时注射等体积生理盐水。连续喂养3个月后取小鼠海马分别进行胶质纤维酸性蛋白(glial fibrillary acidicprotein,GFAP)、CD45和半乳糖脑苷脂(gallc to cerebroside,GC)免疫组化染色,光镜下对海马CA3区星形胶质细胞、小胶质细胞和少突胶质细胞进行形态计量分析,电镜下观察胶质细胞的形态变化。结果:光镜下镉中毒组胶质细胞主要变化有:GFAP和CD45阳性细胞数增多(P<0.05);而GC阳性细胞数减少(P<0.05);电镜下对照组胶质细胞核膜界线清晰,核内染色质分布均匀,线粒体结构完整,嵴清晰,粗面内质网丰富;镉中毒组胶质细胞水肿,线粒体减少,嵴断裂。结论:慢性镉中毒致小鼠海马CA3区星型胶质细胞和小胶质细胞增多,而少突胶质细胞减少,这些变化可能是镉的神经毒性作用之一。展开更多
BACKGROUND: Infantile Krabbe’s disease produces progressive neurologic deteri oration and death in early childhood. We hypothesized that transplantation of um bilical-cord blood from unrelated donors before the devel...BACKGROUND: Infantile Krabbe’s disease produces progressive neurologic deteri oration and death in early childhood. We hypothesized that transplantation of um bilical-cord blood from unrelated donors before the development of symptoms wou ld favorably alter the natural history of the disease among newborns in whom the disease was diagnosed because of a family history. We compared the outcomes amo ng these newborns with the outcomes among infants who underwent transplantation after the development of symptoms and with the outcomes in an untreated cohort o f affected children. METHODS: Eleven asymptomatic newborns (age range, 12 to 44 days) and 14 symptomatic infants (age range, 142 to 352 days) with infantile Kra bbe’s disease underwent transplantation of umbilical-cord blood from unrelated donors after myeloablative chemotherapy. Engraftment, survival, and neurodevelo pmental function were evaluated longitudinally for four months to six years. RES ULTS: The rates of donor-cell engraftment and survival were 100 percent and 100 percent, respectively, among the asymptomatic newborns (median follow-up, 3.0 years) and 100 percent and 43 percent, respectively, among the symptomatic infan ts (median follow-up, 3.4 years). Surviving patients showed durable engraftment of donor-derived hematopoietic cells with restoration of normal blood galactoc erebrosidase levels. Infants who underwent transplantation before the developmen t of symptoms showed progressive central myelination and continued gains in deve lopmental skills, and most had age-appropriate cognitive function and receptive language skills, but a few had mild-to-moderate delays in expressive language and mild-to-severe delays in gross motor function. Children who underwent tra nsplantation after the onset of symptoms had minimal neurologic improvement. CON CLUSIONS: Transplantation of umbilical-cord blood from unrelated donors in newb orns with infantile Krabbe’s disease favorably altered the natural history of t he disease. Transplantation in babies after展开更多
文摘目的:观察慢性镉中毒对小鼠海马CA3区神经胶质细胞的影响。方法:将40只小鼠随机分为对照组与镉中毒组,每组20只。饲养1周后,镉中毒组采用皮下注射氯化镉,每周2次,连续3个月,对照组同时注射等体积生理盐水。连续喂养3个月后取小鼠海马分别进行胶质纤维酸性蛋白(glial fibrillary acidicprotein,GFAP)、CD45和半乳糖脑苷脂(gallc to cerebroside,GC)免疫组化染色,光镜下对海马CA3区星形胶质细胞、小胶质细胞和少突胶质细胞进行形态计量分析,电镜下观察胶质细胞的形态变化。结果:光镜下镉中毒组胶质细胞主要变化有:GFAP和CD45阳性细胞数增多(P<0.05);而GC阳性细胞数减少(P<0.05);电镜下对照组胶质细胞核膜界线清晰,核内染色质分布均匀,线粒体结构完整,嵴清晰,粗面内质网丰富;镉中毒组胶质细胞水肿,线粒体减少,嵴断裂。结论:慢性镉中毒致小鼠海马CA3区星型胶质细胞和小胶质细胞增多,而少突胶质细胞减少,这些变化可能是镉的神经毒性作用之一。
文摘BACKGROUND: Infantile Krabbe’s disease produces progressive neurologic deteri oration and death in early childhood. We hypothesized that transplantation of um bilical-cord blood from unrelated donors before the development of symptoms wou ld favorably alter the natural history of the disease among newborns in whom the disease was diagnosed because of a family history. We compared the outcomes amo ng these newborns with the outcomes among infants who underwent transplantation after the development of symptoms and with the outcomes in an untreated cohort o f affected children. METHODS: Eleven asymptomatic newborns (age range, 12 to 44 days) and 14 symptomatic infants (age range, 142 to 352 days) with infantile Kra bbe’s disease underwent transplantation of umbilical-cord blood from unrelated donors after myeloablative chemotherapy. Engraftment, survival, and neurodevelo pmental function were evaluated longitudinally for four months to six years. RES ULTS: The rates of donor-cell engraftment and survival were 100 percent and 100 percent, respectively, among the asymptomatic newborns (median follow-up, 3.0 years) and 100 percent and 43 percent, respectively, among the symptomatic infan ts (median follow-up, 3.4 years). Surviving patients showed durable engraftment of donor-derived hematopoietic cells with restoration of normal blood galactoc erebrosidase levels. Infants who underwent transplantation before the developmen t of symptoms showed progressive central myelination and continued gains in deve lopmental skills, and most had age-appropriate cognitive function and receptive language skills, but a few had mild-to-moderate delays in expressive language and mild-to-severe delays in gross motor function. Children who underwent tra nsplantation after the onset of symptoms had minimal neurologic improvement. CON CLUSIONS: Transplantation of umbilical-cord blood from unrelated donors in newb orns with infantile Krabbe’s disease favorably altered the natural history of t he disease. Transplantation in babies after
