Objective:To investigate the histopathologic changes of hypothalamus and pituitary in a rat model of polycystic ovary syndrome(PCOS). Methods:Rat model of PCOS was established in 6 immature female SD rats and another ...Objective:To investigate the histopathologic changes of hypothalamus and pituitary in a rat model of polycystic ovary syndrome(PCOS). Methods:Rat model of PCOS was established in 6 immature female SD rats and another 6 immature rats treated with placebo were as control.The tissues of hypothalamus and pituitary were obtained and observed by light microscope and transmission electron microscope. Results:Light microscopy revealed little difference in morphology of neurons in arcuate nucleus or basophilic cells in pituitary between PCOS rats and normal rats.Electron microscopic examination showed that,compared with those in normal rats,GnRH neurons in PCOS rats were larger and fuller,with Golgi complex and mitochondria increased. The mitochondria were small,round and swelling.More high-density secretory granules and bright vesicles were observed in the cytoplasm.The Golgi complex near nucleus in pituitary gonadotropin cell in PCOS rats was fractured and expanded,and there were increased number mitochondria and different sizes of the higher electron density secretory granules in the cytoplasm. Conclusion:The morphological alterations in hypothalamus and pituitary could play a very important role in the development of PCOS.展开更多
文摘Objective:To investigate the histopathologic changes of hypothalamus and pituitary in a rat model of polycystic ovary syndrome(PCOS). Methods:Rat model of PCOS was established in 6 immature female SD rats and another 6 immature rats treated with placebo were as control.The tissues of hypothalamus and pituitary were obtained and observed by light microscope and transmission electron microscope. Results:Light microscopy revealed little difference in morphology of neurons in arcuate nucleus or basophilic cells in pituitary between PCOS rats and normal rats.Electron microscopic examination showed that,compared with those in normal rats,GnRH neurons in PCOS rats were larger and fuller,with Golgi complex and mitochondria increased. The mitochondria were small,round and swelling.More high-density secretory granules and bright vesicles were observed in the cytoplasm.The Golgi complex near nucleus in pituitary gonadotropin cell in PCOS rats was fractured and expanded,and there were increased number mitochondria and different sizes of the higher electron density secretory granules in the cytoplasm. Conclusion:The morphological alterations in hypothalamus and pituitary could play a very important role in the development of PCOS.