A sensitive and specific reversed-phase high performance liquid chromatography （RP-HPLC） method was established for the simultaneous quantitative determination of eight active components, two stilbenes （resveratrol...A sensitive and specific reversed-phase high performance liquid chromatography （RP-HPLC） method was established for the simultaneous quantitative determination of eight active components, two stilbenes （resveratrol, polydatin） and six flavonoids （rutin, quercilrin, quercetin, luteotin, isoorientin, kaempferol）, in Polygonum multiflorum Thunb. The chromatographic separation was performed on a Kromasil C18 column （250 mm^4.6 mm, 5 ~tm）, and gradient elution was carried out with water-methanol as the mobile phase at a flow rate of 1 mL/min. Stilbenes and flavonoids were respectively detected at 320 nm and 350 nm with DAD. The correlation coefficients of all the calibration curves were found to be higher than 0.9995. The average recoveries were ranged from 96.8% to 102.5% with RSD less than 4.8% for these components. RP-HPLC was validated to be a robust method for the quantitative determination of active components in Polygonum multiflorum Thunb, and it could be used in the quality control of this traditional medicine.展开更多
桑树(Morus alba L.)根的乙醇浸膏具有抑制蛋白激酶C(PKC)的活性,从中分得的二种二苯乙烯类衍生物:oxyresveratrol和kuwanon Y对PKC的半抑制浓度IC_(50)分别为48μmol·L^(-1)和15μmol·L^(-1)。酶动力学研究显示,oxyresverat...桑树(Morus alba L.)根的乙醇浸膏具有抑制蛋白激酶C(PKC)的活性,从中分得的二种二苯乙烯类衍生物:oxyresveratrol和kuwanon Y对PKC的半抑制浓度IC_(50)分别为48μmol·L^(-1)和15μmol·L^(-1)。酶动力学研究显示,oxyresveratrol对PKC的抑制属于非竞争性抑制。展开更多
文摘A sensitive and specific reversed-phase high performance liquid chromatography （RP-HPLC） method was established for the simultaneous quantitative determination of eight active components, two stilbenes （resveratrol, polydatin） and six flavonoids （rutin, quercilrin, quercetin, luteotin, isoorientin, kaempferol）, in Polygonum multiflorum Thunb. The chromatographic separation was performed on a Kromasil C18 column （250 mm^4.6 mm, 5 ~tm）, and gradient elution was carried out with water-methanol as the mobile phase at a flow rate of 1 mL/min. Stilbenes and flavonoids were respectively detected at 320 nm and 350 nm with DAD. The correlation coefficients of all the calibration curves were found to be higher than 0.9995. The average recoveries were ranged from 96.8% to 102.5% with RSD less than 4.8% for these components. RP-HPLC was validated to be a robust method for the quantitative determination of active components in Polygonum multiflorum Thunb, and it could be used in the quality control of this traditional medicine.
文摘桑树(Morus alba L.)根的乙醇浸膏具有抑制蛋白激酶C(PKC)的活性,从中分得的二种二苯乙烯类衍生物:oxyresveratrol和kuwanon Y对PKC的半抑制浓度IC_(50)分别为48μmol·L^(-1)和15μmol·L^(-1)。酶动力学研究显示,oxyresveratrol对PKC的抑制属于非竞争性抑制。
