采用盆栽称量法研究了对照、中度和重度干旱(土壤相对含水量分别为75%、55%和35%)条件下1年生酸枣〔Ziziphus jujuba Mill.var.spinosa(Bunge)Hu ex H.F.Chow〕幼苗叶片中黄酮类成分含量及一些生长和生理指标的变化,并讨论了酸枣适应干...采用盆栽称量法研究了对照、中度和重度干旱(土壤相对含水量分别为75%、55%和35%)条件下1年生酸枣〔Ziziphus jujuba Mill.var.spinosa(Bunge)Hu ex H.F.Chow〕幼苗叶片中黄酮类成分含量及一些生长和生理指标的变化,并讨论了酸枣适应干旱过程中黄酮类成分的作用。结果表明:在中度和重度干旱条件下酸枣叶片苯丙氨酸解氨酶(PAL)活性均高于对照,但经重度干旱处理后复水第7天PAL活性与对照无显著差异。与对照相比,中度干旱条件下叶片槲皮素含量基本无变化,总黄酮和芦丁含量分别显著或极显著增加,丙二醛和可溶性蛋白质含量无显著变化;与中度干旱条件相比,重度干旱条件下叶片总黄酮、芦丁和槲皮素含量显著或极显著下降,丙二醛含量不显著增加,可溶性蛋白质含量显著降低;与对照相比,重度干旱条件下总黄酮含量无明显变化,芦丁和丙二醛含量极显著或显著增加,槲皮素和可溶性蛋白质含量显著下降;复水第7天,总黄酮、芦丁和槲皮素含量均显著或极显著高于复水前,且总黄酮和芦丁含量显著高于对照,槲皮素含量与对照无显著差异;复水第1天至第4天,丙二醛含量呈先降后增再降的趋势、可溶性蛋白质含量则呈先降低后逐渐增加的趋势,其中复水第1天丙二醛含量显著高于复水前、可溶性蛋白质含量显著低于复水前。在中度或重度干旱胁迫后酸枣枝条长度均极显著小于对照,且随土壤相对含水量的降低枝条长度减小;叶片相对含水量也表现出随土壤相对含水量的降低逐渐减小的趋势,但差异不显著。研究结果提示:适宜的干旱胁迫可促进酸枣叶片黄酮类代谢,但在不同的干旱胁迫条件下,黄酮类代谢在酸枣抗旱过程中具有不同的作用。展开更多
Genetic relationships between Ziziphus jujuba and Ziziphus spinosa populations were studied using RAPD data in population genetics. 275 loci were gained among which 249 loci were polymorphic by 22 primers. The polymor...Genetic relationships between Ziziphus jujuba and Ziziphus spinosa populations were studied using RAPD data in population genetics. 275 loci were gained among which 249 loci were polymorphic by 22 primers. The polymorphic loci percentage was 89% among Z.spinosa population while that of Z.jujuba population was 56%. 31 specific RAPD markers were detected on 3 Z.jujuba varieties and 15 Z.spinosa forms. Cluster analysis showed: when λ=12, the forms tested were divided into 2 groups. One included Z. jujuba cv. Zanhuangdazao, Yudichuangan, Yuanshichuangan, Xiaochuangan, Qiantai No.1, Luzhuangchuangan; the other included Z. jujuba cv. Zilingdan and 26 Z. spinosa forms. All above indicated that Z. jujuba and Z.spinosa should be regarded as two separate species.展开更多
The leaf anatomic structure of %Zizyphus jujuba% var. %spinosa% from seashore to western arid area (near 37° N latitude) of China were investigated with both a light microscope and a scanning electron microscope....The leaf anatomic structure of %Zizyphus jujuba% var. %spinosa% from seashore to western arid area (near 37° N latitude) of China were investigated with both a light microscope and a scanning electron microscope. The results showed that all provenances shared the following common features. The distribution of stomata was generally uniform on the abaxial surface, and the type of stomatal apparatus was mainly anomocytic. The upper epidermis was formed by one layer of cells with tight, regular arrangement, and the lower epidermis was also formed by one layer of cells, and the type of anticlinal cell wall on both the upper and on the lower epidermis was straight. In the contrast, there were some differences in the anatomic characteristics among the provenances. With the decrease of water content in the habitats, (1) the stomatas became smaller gradually, and the density of stomata increase gradually; (2) the cells of both the upper and the lower epidermis became smaller gradually; (3) thickness of the upper and the lower cuticle, as well as the thickness of leaf, increased gradually; (4) bifacial leaf changed to isobilateral leaf; (5) the proportion of palisade tissue increased gradually, whereas the spongy tissue decreased; (6) diameter of midrib vessels and of midrib vascular bundles increased gradually; (7) the epidermis ornament changed in an order of smoothness, strip, reticulation, and mountain-like that represented the leaf structural characteristics of xerophytes. The results also showed that the sequence of the drought resistance delicacy was thickness of upper cuticle>density of stomata in proximal surface, size of stomata in proximal surface (length/width), thickness of lower cuticle, thickness of palisade, and thickness of spongy>average length of stomata, average length/width of upper epidermis cells, thickness of leaf, value of CTR, thickness of upper epiderm cell, thickness of lower epidermic cell, layers of palisade>diameter of midrib vascular bundle, average width of stomata, the area of stomata in 展开更多
文摘采用盆栽称量法研究了对照、中度和重度干旱(土壤相对含水量分别为75%、55%和35%)条件下1年生酸枣〔Ziziphus jujuba Mill.var.spinosa(Bunge)Hu ex H.F.Chow〕幼苗叶片中黄酮类成分含量及一些生长和生理指标的变化,并讨论了酸枣适应干旱过程中黄酮类成分的作用。结果表明:在中度和重度干旱条件下酸枣叶片苯丙氨酸解氨酶(PAL)活性均高于对照,但经重度干旱处理后复水第7天PAL活性与对照无显著差异。与对照相比,中度干旱条件下叶片槲皮素含量基本无变化,总黄酮和芦丁含量分别显著或极显著增加,丙二醛和可溶性蛋白质含量无显著变化;与中度干旱条件相比,重度干旱条件下叶片总黄酮、芦丁和槲皮素含量显著或极显著下降,丙二醛含量不显著增加,可溶性蛋白质含量显著降低;与对照相比,重度干旱条件下总黄酮含量无明显变化,芦丁和丙二醛含量极显著或显著增加,槲皮素和可溶性蛋白质含量显著下降;复水第7天,总黄酮、芦丁和槲皮素含量均显著或极显著高于复水前,且总黄酮和芦丁含量显著高于对照,槲皮素含量与对照无显著差异;复水第1天至第4天,丙二醛含量呈先降后增再降的趋势、可溶性蛋白质含量则呈先降低后逐渐增加的趋势,其中复水第1天丙二醛含量显著高于复水前、可溶性蛋白质含量显著低于复水前。在中度或重度干旱胁迫后酸枣枝条长度均极显著小于对照,且随土壤相对含水量的降低枝条长度减小;叶片相对含水量也表现出随土壤相对含水量的降低逐渐减小的趋势,但差异不显著。研究结果提示:适宜的干旱胁迫可促进酸枣叶片黄酮类代谢,但在不同的干旱胁迫条件下,黄酮类代谢在酸枣抗旱过程中具有不同的作用。
文摘Genetic relationships between Ziziphus jujuba and Ziziphus spinosa populations were studied using RAPD data in population genetics. 275 loci were gained among which 249 loci were polymorphic by 22 primers. The polymorphic loci percentage was 89% among Z.spinosa population while that of Z.jujuba population was 56%. 31 specific RAPD markers were detected on 3 Z.jujuba varieties and 15 Z.spinosa forms. Cluster analysis showed: when λ=12, the forms tested were divided into 2 groups. One included Z. jujuba cv. Zanhuangdazao, Yudichuangan, Yuanshichuangan, Xiaochuangan, Qiantai No.1, Luzhuangchuangan; the other included Z. jujuba cv. Zilingdan and 26 Z. spinosa forms. All above indicated that Z. jujuba and Z.spinosa should be regarded as two separate species.
文摘The leaf anatomic structure of %Zizyphus jujuba% var. %spinosa% from seashore to western arid area (near 37° N latitude) of China were investigated with both a light microscope and a scanning electron microscope. The results showed that all provenances shared the following common features. The distribution of stomata was generally uniform on the abaxial surface, and the type of stomatal apparatus was mainly anomocytic. The upper epidermis was formed by one layer of cells with tight, regular arrangement, and the lower epidermis was also formed by one layer of cells, and the type of anticlinal cell wall on both the upper and on the lower epidermis was straight. In the contrast, there were some differences in the anatomic characteristics among the provenances. With the decrease of water content in the habitats, (1) the stomatas became smaller gradually, and the density of stomata increase gradually; (2) the cells of both the upper and the lower epidermis became smaller gradually; (3) thickness of the upper and the lower cuticle, as well as the thickness of leaf, increased gradually; (4) bifacial leaf changed to isobilateral leaf; (5) the proportion of palisade tissue increased gradually, whereas the spongy tissue decreased; (6) diameter of midrib vessels and of midrib vascular bundles increased gradually; (7) the epidermis ornament changed in an order of smoothness, strip, reticulation, and mountain-like that represented the leaf structural characteristics of xerophytes. The results also showed that the sequence of the drought resistance delicacy was thickness of upper cuticle>density of stomata in proximal surface, size of stomata in proximal surface (length/width), thickness of lower cuticle, thickness of palisade, and thickness of spongy>average length of stomata, average length/width of upper epidermis cells, thickness of leaf, value of CTR, thickness of upper epiderm cell, thickness of lower epidermic cell, layers of palisade>diameter of midrib vascular bundle, average width of stomata, the area of stomata in
