采用RT-PCR和RACE(rapid amplification of cDNA ends)技术,从红花未成熟的种子和叶片中分离到8个FAD2基因和1个FAD6基因片段,并全部提交至GenBank上。在NCBI中Blast结果显示,CtFAD2-1和Ct-FAD2-8与向日葵、大豆、棉花等种子特异表达的F...采用RT-PCR和RACE(rapid amplification of cDNA ends)技术,从红花未成熟的种子和叶片中分离到8个FAD2基因和1个FAD6基因片段,并全部提交至GenBank上。在NCBI中Blast结果显示,CtFAD2-1和Ct-FAD2-8与向日葵、大豆、棉花等种子特异表达的FAD2-1基因的同源性较高。而CtFAD2-2与其他作物中组成型表达的FAD2-2/CtFAD2-3有较高的相似性。将红花ω6脂肪酸脱氢酶氨基酸序列进行同源性比对,CtFAD2-1与CtFAD2-8的同源性最高,为79.9%;其次是CtFAD2-2与CtFAD2-1和CtFAD2-8,分别为70.8%和70.2%;而这3个FAD2基因与其余5个拷贝间的序列相似性均较低,在52.0%～61.0%之间;CtFAD6与CtFAD2之间的相似性极低,在18.0%～21.9%之间。红花8个FAD2均检测到内质网滞留信号,将其定位于内质网膜上,而CtFAD6 N-端检测到67bp的质体信号肽序列,将其定位于叶绿体膜上。疏水性和跨膜分析结果显示,除Ct-FAD2-6以外,其余FAD2均含有6个疏水区,分别跨膜4～6次。展开更多
The feeding trial was conducted to determine the effects of flaxseed on layer＇s performance and egg quality, and the effects of the dietary ratio of ω-6 fatty acid to ω-3 fatty acid on fatty acid deposition in yolk...The feeding trial was conducted to determine the effects of flaxseed on layer＇s performance and egg quality, and the effects of the dietary ratio of ω-6 fatty acid to ω-3 fatty acid on fatty acid deposition in yolk using flaxseed as resource of ω-3 fatty acid and sunflower seed as resource of ω-6 fatty acid. 192 20-wk-old Newroman commercial hens were randomly divided into 4 treatment groups with 6 replicates containing 8 birds. The rations corresponding with the four treatments were formulated with 4% flaxseed ＋ 9% sunflower seed, 8% flaxseed ＋ 18% sunflower seed, 15% flaxseed, and soybean meal, respectively. Sunflower seed was unshelled and flaxseed was whole. Ratios of ω-6 fatty acid to ω-3 fatty acid in 4 dietary treatments were 5.67, 5.21, 1.07, and 11.83, respectively. It can be concluded that long-term feeding 15% flaxseed had no significant （P〉 0.05） influence on egg production, egg weight, eggshell quality, yolk weight, and percentage of yolk weight. Dietary ratio of ω-6 fatty acid to ω-3 fatty acid had impact on the 0-3 fatty acid levels in yolk, nevertheless yolk fatty acid profiles were different from those of dietary fatty acid compositions.展开更多
The main aim of this research was to identify fatty acids composition of Caspian Sea of white fish Rutilusfrisii kutum tissue and their changes during 12 months storage （-18 ℃ ）. The results showed, UFA （Unsaturat...The main aim of this research was to identify fatty acids composition of Caspian Sea of white fish Rutilusfrisii kutum tissue and their changes during 12 months storage （-18 ℃ ）. The results showed, UFA （Unsaturated Fatty Acid） and SFA （Saturated Fatty Acid） were 74/09 and 21/63%, respectively in fresh tissue. So that, DHA （C22：6） oleic acid （C 18：1c） had high amounts （15/07, 20/57） UFA and palmitic acid （C16：0） was the most （13/09%） SFA. The effects of freezing on fish tissue showed that UFA and SFA contents have reached to 58/79 and 22/17%, respectively at the end of cold storage. Also ω-3 and ω-6 series of fatty acids was 24/22 and 15/56% in fresh tissue, but their contents decreased to 8/68 and 5/11% at the end of period. Among, the fatty acids C22：6, C 18：1 c and C 16：0 had the most changes. The changes of fatty acids were significantly at 95% level expected for C18：0.展开更多
基金supported by the Heilongjiang Province Government Foundation,China(HL-98)
文摘The feeding trial was conducted to determine the effects of flaxseed on layer＇s performance and egg quality, and the effects of the dietary ratio of ω-6 fatty acid to ω-3 fatty acid on fatty acid deposition in yolk using flaxseed as resource of ω-3 fatty acid and sunflower seed as resource of ω-6 fatty acid. 192 20-wk-old Newroman commercial hens were randomly divided into 4 treatment groups with 6 replicates containing 8 birds. The rations corresponding with the four treatments were formulated with 4% flaxseed ＋ 9% sunflower seed, 8% flaxseed ＋ 18% sunflower seed, 15% flaxseed, and soybean meal, respectively. Sunflower seed was unshelled and flaxseed was whole. Ratios of ω-6 fatty acid to ω-3 fatty acid in 4 dietary treatments were 5.67, 5.21, 1.07, and 11.83, respectively. It can be concluded that long-term feeding 15% flaxseed had no significant （P〉 0.05） influence on egg production, egg weight, eggshell quality, yolk weight, and percentage of yolk weight. Dietary ratio of ω-6 fatty acid to ω-3 fatty acid had impact on the 0-3 fatty acid levels in yolk, nevertheless yolk fatty acid profiles were different from those of dietary fatty acid compositions.
文摘The main aim of this research was to identify fatty acids composition of Caspian Sea of white fish Rutilusfrisii kutum tissue and their changes during 12 months storage （-18 ℃ ）. The results showed, UFA （Unsaturated Fatty Acid） and SFA （Saturated Fatty Acid） were 74/09 and 21/63%, respectively in fresh tissue. So that, DHA （C22：6） oleic acid （C 18：1c） had high amounts （15/07, 20/57） UFA and palmitic acid （C16：0） was the most （13/09%） SFA. The effects of freezing on fish tissue showed that UFA and SFA contents have reached to 58/79 and 22/17%, respectively at the end of cold storage. Also ω-3 and ω-6 series of fatty acids was 24/22 and 15/56% in fresh tissue, but their contents decreased to 8/68 and 5/11% at the end of period. Among, the fatty acids C22：6, C 18：1 c and C 16：0 had the most changes. The changes of fatty acids were significantly at 95% level expected for C18：0.
