AIM: To investigate the expression pattern of γ-synuclein in colorectal cancer (CRC) tissues, and to study the effects of γ-synuclein on CRC cell line HCT116 biological features in vitro.METHODS: The expression patt...AIM: To investigate the expression pattern of γ-synuclein in colorectal cancer (CRC) tissues, and to study the effects of γ-synuclein on CRC cell line HCT116 biological features in vitro.METHODS: The expression pattern of γ-synuclein was determined in 54 CRC tissues and 30 tumor-matched nonneoplastic adjacent tissues (NNAT) 5 cm away from the tumor via real-time quantitative reverse transcription PCR (RT-PCR) and immunohistochemistry. The relationship between γ-synuclein protein expression and clinicopathological factors of CRC tissues was analyzed. Three small interfering RNA (siRNA) targeting γ-synuclein mRNA plasmids were constructed and transfected into the CRC cell line HCT116. The stable cell lines were selected with G-418 for 28 d, and the biological features of these cells were examined by cell growth curve, soft agar assay, and cell migration and invasion assays in vitro. RESULTS: The expression of γ-synuclein mRNA and protein was much higher in CRC tissue samples than in NNAT samples (P = 0.02, P = 0.036). There was a significant correlation between the γ-synuclein protein expression and clinical stage and lymph node involvement of CRC (P = 0.02, P = 0.033). In functional analysis we found that down-regulation of γ-synuclein expression in HCT116 cells could inhibit the growth, colony formation rate, and migration and invasion ability of HCT116 cells.CONCLUSION: Increased expression of γ-synuclein in CRC tissues and the biological effects of reduced γ-synuclein expression on HCT116 cells suggest that γ-synuclein may play a positive role in the progression of CRC.展开更多
γ-突触核蛋白基因（γ-synuclein，SNCG），又称乳腺癌特异性基因1（breast cancer specific gene1，BCSG1），由Ji等于1997年发现，与已知的α-和β-突触核蛋白同属突触核蛋白基因家族。对突触核蛋白家族的研究一直是神经变性疾病领...γ-突触核蛋白基因（γ-synuclein，SNCG），又称乳腺癌特异性基因1（breast cancer specific gene1，BCSG1），由Ji等于1997年发现，与已知的α-和β-突触核蛋白同属突触核蛋白基因家族。对突触核蛋白家族的研究一直是神经变性疾病领域的热点，它的表达异常影响多巴胺能神经元功能，导致阿尔茨海默病和帕金森病等神经退行性疾病的发生和发展。近期研究发现突触核蛋白家族与肿瘤遗传学相关，尤其是其中最后发现的SNCG与多种器官恶性肿瘤的发生和进展有关。在乳腺癌中，SNCG可能与肿瘤发生、进展、转移以及化疗耐药关系密切。展开更多
文摘AIM: To investigate the expression pattern of γ-synuclein in colorectal cancer (CRC) tissues, and to study the effects of γ-synuclein on CRC cell line HCT116 biological features in vitro.METHODS: The expression pattern of γ-synuclein was determined in 54 CRC tissues and 30 tumor-matched nonneoplastic adjacent tissues (NNAT) 5 cm away from the tumor via real-time quantitative reverse transcription PCR (RT-PCR) and immunohistochemistry. The relationship between γ-synuclein protein expression and clinicopathological factors of CRC tissues was analyzed. Three small interfering RNA (siRNA) targeting γ-synuclein mRNA plasmids were constructed and transfected into the CRC cell line HCT116. The stable cell lines were selected with G-418 for 28 d, and the biological features of these cells were examined by cell growth curve, soft agar assay, and cell migration and invasion assays in vitro. RESULTS: The expression of γ-synuclein mRNA and protein was much higher in CRC tissue samples than in NNAT samples (P = 0.02, P = 0.036). There was a significant correlation between the γ-synuclein protein expression and clinical stage and lymph node involvement of CRC (P = 0.02, P = 0.033). In functional analysis we found that down-regulation of γ-synuclein expression in HCT116 cells could inhibit the growth, colony formation rate, and migration and invasion ability of HCT116 cells.CONCLUSION: Increased expression of γ-synuclein in CRC tissues and the biological effects of reduced γ-synuclein expression on HCT116 cells suggest that γ-synuclein may play a positive role in the progression of CRC.
文摘γ-突触核蛋白基因（γ-synuclein，SNCG），又称乳腺癌特异性基因1（breast cancer specific gene1，BCSG1），由Ji等于1997年发现，与已知的α-和β-突触核蛋白同属突触核蛋白基因家族。对突触核蛋白家族的研究一直是神经变性疾病领域的热点，它的表达异常影响多巴胺能神经元功能，导致阿尔茨海默病和帕金森病等神经退行性疾病的发生和发展。近期研究发现突触核蛋白家族与肿瘤遗传学相关，尤其是其中最后发现的SNCG与多种器官恶性肿瘤的发生和进展有关。在乳腺癌中，SNCG可能与肿瘤发生、进展、转移以及化疗耐药关系密切。
