Background： Previous studies suggested that supplementation of lactating sows with β-hydroxy-β-methylbutyrate（HMB） could improve the performance of weaning pigs, but there were little information in the muscle fi...Background： Previous studies suggested that supplementation of lactating sows with β-hydroxy-β-methylbutyrate（HMB） could improve the performance of weaning pigs, but there were little information in the muscle fiber type transformation of the offspring and the subsequent performance in pigs from weaning through finishing in response to maternal HMB consumption. The purpose of this study was to determine the effect of supplementing lactating sows with HMB on skeletal muscle fiber type transformation and growth of the offspring during d 28 and180 after birth. A total of 20 sows according to their body weight were divided into the control（CON, n = 10） or HMB groups（HMB, n = 10）. Sows in the HMB group were supplemented with β-hydroxy-β-methylbutyrate calcium（HMB-Ca） 2 g/kg feed during d 1 to 27 of lactation. After weaning, 48 mixed sex piglets were blocked by sow treatment and fed standard diets for post-weaning, growing, finishing periods. Growth performance was recorded during d 28 to 180 after birth. Pigs were slaughtered on d 28（n = 6/treatment） and 180（n = 6/treatment） postnatal and the longissimus dorsi（LD） was collected, respectively.Results： The HMB-fed sows during lactation showed increased HMB concentration（P ＆lt; 0.05） in milk and LD of weaning piglets（P ＆lt; 0.05）. In addition, offsprings in HMB group had a higher finishing BW and lean percentage than did pigs in CON group（P ＆lt; 0.05）, meanwhile, compared with pigs from sows fed the CON diet, pigs from sows fed HMB diet showed higher type Ⅱ muscle fiber cross-sectional area（CSA）, elevated myosin heavy chain（MyHC） Ⅱb and Sox6 mRNA, and fast-MyHC protein levels in LD（P ＆lt; 0.05）.Conclusions： HMB supplemented to sow diets throughout lactation increases the levels of HMB in maternal milk and skeletal muscle of pigs during d 28 after birth and promotes subsequent performance of pigs between d 28 and 180 of age by enhancing glycolytic muscle fiber transformation.展开更多
本试验旨在研究胚蛋给养（in ovo feeding）β-羟基-β-甲基丁酸（beta-hydroxy-beta-methylbutyrate,HMB）对肉仔鸡孵化率、生长性能及骨骼肌发育的影响。选用540枚商品代AA肉仔鸡受精蛋,随机分为3个组：空白组（不注射）、生理盐水组...本试验旨在研究胚蛋给养（in ovo feeding）β-羟基-β-甲基丁酸（beta-hydroxy-beta-methylbutyrate,HMB）对肉仔鸡孵化率、生长性能及骨骼肌发育的影响。选用540枚商品代AA肉仔鸡受精蛋,随机分为3个组：空白组（不注射）、生理盐水组（7胚龄气室注射1mL 0.9%生理盐水）、HMB组（7胚龄气室注射1mL含0.1%HMB的生理盐水）。出壳后,每组选取72只体重相近健康公雏随机分成6个重复,每重复12只。结果表明：1）胚蛋给养对种蛋孵化率无显著性影响（P〉0.05）;2）HMB组出壳重、2l日龄体重、0~21日龄平均日增重均显著高于其他组（P〈0.05）;3）7日龄时,HMB组肉仔鸡胸肌率较生理盐水组提高了1.01%（P=0.019）;21日龄时,HMB组胸肌率显著高于其他组（P〈0.05）,较空白组和生理盐水组分别提高了1.11%和1.04%;4）HMB组肉仔鸡的腹脂率最低,且在21日龄时显著低于空白组（P=0.018）;5）与空白组和生理盐水组相比,HMB组肉仔鸡出壳当天和4日龄肌细胞直径显著增大（P〈0.05）。6）出壳当天和7日龄肉仔鸡,HMB组胸肌卫星细胞有丝分裂活性指数较其他两组显著提高（P〈0.05）;7）7日龄肉仔鸡,HMB组血浆IGF-1含量显著高于空白组（P〈0.05）,与生理盐水组之间差异不显著（P〉0.05）。结果提示,胚蛋给养HMB可提高肉仔鸡出壳重,增加卫星细胞有丝分裂活性,促进肉鸡胸肌发育,加快肉鸡前期生长;注射HMB不影响种蛋孵化率和肉鸡饲料转化效率;注射生理盐水与不注射处理结果相似,对肉仔鸡生长没有影响。展开更多
基金supported by the National Special Research Fund for Non-Profit Sector(Agriculture)(No.201203015)Academy of Kechuang Feed Industry in Sichuan(2013NZ0056)+1 种基金Research Team of Youth Scientific and Technical Innovation of Sichuan(13CXTD0004)the Program for Changjiang Scholars and Innovative Research Team in University(IRT13083)
文摘Background： Previous studies suggested that supplementation of lactating sows with β-hydroxy-β-methylbutyrate（HMB） could improve the performance of weaning pigs, but there were little information in the muscle fiber type transformation of the offspring and the subsequent performance in pigs from weaning through finishing in response to maternal HMB consumption. The purpose of this study was to determine the effect of supplementing lactating sows with HMB on skeletal muscle fiber type transformation and growth of the offspring during d 28 and180 after birth. A total of 20 sows according to their body weight were divided into the control（CON, n = 10） or HMB groups（HMB, n = 10）. Sows in the HMB group were supplemented with β-hydroxy-β-methylbutyrate calcium（HMB-Ca） 2 g/kg feed during d 1 to 27 of lactation. After weaning, 48 mixed sex piglets were blocked by sow treatment and fed standard diets for post-weaning, growing, finishing periods. Growth performance was recorded during d 28 to 180 after birth. Pigs were slaughtered on d 28（n = 6/treatment） and 180（n = 6/treatment） postnatal and the longissimus dorsi（LD） was collected, respectively.Results： The HMB-fed sows during lactation showed increased HMB concentration（P ＆lt; 0.05） in milk and LD of weaning piglets（P ＆lt; 0.05）. In addition, offsprings in HMB group had a higher finishing BW and lean percentage than did pigs in CON group（P ＆lt; 0.05）, meanwhile, compared with pigs from sows fed the CON diet, pigs from sows fed HMB diet showed higher type Ⅱ muscle fiber cross-sectional area（CSA）, elevated myosin heavy chain（MyHC） Ⅱb and Sox6 mRNA, and fast-MyHC protein levels in LD（P ＆lt; 0.05）.Conclusions： HMB supplemented to sow diets throughout lactation increases the levels of HMB in maternal milk and skeletal muscle of pigs during d 28 after birth and promotes subsequent performance of pigs between d 28 and 180 of age by enhancing glycolytic muscle fiber transformation.
文摘本试验旨在研究胚蛋给养（in ovo feeding）β-羟基-β-甲基丁酸（beta-hydroxy-beta-methylbutyrate,HMB）对肉仔鸡孵化率、生长性能及骨骼肌发育的影响。选用540枚商品代AA肉仔鸡受精蛋,随机分为3个组：空白组（不注射）、生理盐水组（7胚龄气室注射1mL 0.9%生理盐水）、HMB组（7胚龄气室注射1mL含0.1%HMB的生理盐水）。出壳后,每组选取72只体重相近健康公雏随机分成6个重复,每重复12只。结果表明：1）胚蛋给养对种蛋孵化率无显著性影响（P〉0.05）;2）HMB组出壳重、2l日龄体重、0~21日龄平均日增重均显著高于其他组（P〈0.05）;3）7日龄时,HMB组肉仔鸡胸肌率较生理盐水组提高了1.01%（P=0.019）;21日龄时,HMB组胸肌率显著高于其他组（P〈0.05）,较空白组和生理盐水组分别提高了1.11%和1.04%;4）HMB组肉仔鸡的腹脂率最低,且在21日龄时显著低于空白组（P=0.018）;5）与空白组和生理盐水组相比,HMB组肉仔鸡出壳当天和4日龄肌细胞直径显著增大（P〈0.05）。6）出壳当天和7日龄肉仔鸡,HMB组胸肌卫星细胞有丝分裂活性指数较其他两组显著提高（P〈0.05）;7）7日龄肉仔鸡,HMB组血浆IGF-1含量显著高于空白组（P〈0.05）,与生理盐水组之间差异不显著（P〉0.05）。结果提示,胚蛋给养HMB可提高肉仔鸡出壳重,增加卫星细胞有丝分裂活性,促进肉鸡胸肌发育,加快肉鸡前期生长;注射HMB不影响种蛋孵化率和肉鸡饲料转化效率;注射生理盐水与不注射处理结果相似,对肉仔鸡生长没有影响。
