Interferon, a natural protein that is produced by a variety of cells during viral infection, activates the transcription of multiple functional genes in cells, regulates synergy among various signaling pathways, and m...Interferon, a natural protein that is produced by a variety of cells during viral infection, activates the transcription of multiple functional genes in cells, regulates synergy among various signaling pathways, and mediates many biological functions such as antiviral activity, immune regulation, and cell growth. However, clinical research on interferon in livestock is lacking. In this study, recombinant porcine interferon(PoIFNa) was used as an adjuvant, in combination with inactivated influenza virus, to vaccinate 6-week-old pigs via nasal infusion. The transcription of target genes was then monitored and the functions of PoIFNa were determined with respect to the activation of mucosal immunity. We found that a combination of low-dose PoIFNa and inactivated influenza virus could significantly up-regulate the expression of immunoregulatory cytokines such as IL-2, IL-18, IFN-c, IL-6, and IL-10 by real-time PCR, suggesting the induction of a strong mucosal innate immune response after administration. In addition, low-dose PoIFNa can significant enhancing the transcription of genes encoding homing factors including CCR9 and CCR10(P \ 0.001), thereby resulting in the induction of higher levels of HA-specific antibodies(P \ 0.05), which can be determined by ELISA and IFA. Post-immunization challenges with H1 N1 virus demonstrated that PoIFNa, combined with inactivated influenza virus, could alleviate clinical signs in pigs during the early stages of viral infection. These studies reveal low-dose PoIFNa as a potential mucosal adjuvant for influenza virus in pigs.展开更多
目的观察甘氨双唑钠(CMNa)联合时辰放疗对鼻咽癌裸鼠移植瘤的时辰放射增敏作用,并探讨其作用机制。方法将荷瘤裸鼠随机分为3组:放疗组、放疗+CMNa组、空白对照组,每组分3HALO(光照后小时,hours after light onset)、9HALO、15HALO、21H...目的观察甘氨双唑钠(CMNa)联合时辰放疗对鼻咽癌裸鼠移植瘤的时辰放射增敏作用,并探讨其作用机制。方法将荷瘤裸鼠随机分为3组:放疗组、放疗+CMNa组、空白对照组,每组分3HALO(光照后小时,hours after light onset)、9HALO、15HALO、21HALO四个时辰进行相应处理。测定肿瘤再生长延长时间(regrowth delay time,TGD),绘制生长曲线。用免疫组化法检测各组肿瘤标本中HIF-1α、γ-H2AX和凋亡蛋白的表达。结果通过对各组TGD的比较,以放疗+CMNa组对肿瘤的抑制效果最好。在该组中,TGD:15HALO>21HALO>9HALO>3HALO,15HALO与3HALO的TGD比较有统计学意义;15HALO与3HALO的HIF-1 α、γ-H2AX及凋亡蛋白的表达水平比较有统计学意义。结论甘氨双唑钠联合时辰放疗对鼻咽癌裸鼠移植瘤有明显的时辰放射增敏作用,以15HALO放疗+CMNa组对肿瘤的抑制效果最佳。其机制可能与HIF-1α的表达,DNA双链损伤,凋亡有关。展开更多
In this paper,we study a boundedness property of the Adams type for multilinear fractional integral operators with the multilinear L^(r′,α)-Hörmander condition and their commutators with vector valued BMO funct...In this paper,we study a boundedness property of the Adams type for multilinear fractional integral operators with the multilinear L^(r′,α)-Hörmander condition and their commutators with vector valued BMO functions on a Morrey space and a predual Morrey space.Moreover,we give an endpoint estimate for multilinear fractional integral operators.As an application,we obtain the boundedness of multilinear Fourier multipliers with limited Sobolev regularity on a Morrey space.展开更多
There is a classic experiential rule about 1,2-diol RCR^1 （OH） CR^1 （OH） R（ R^1 = H or methyl）, that is, the proton chemical shift of the α-R^1 group at the hydroxvl group in the all-isomer, in general, aPPears...There is a classic experiential rule about 1,2-diol RCR^1 （OH） CR^1 （OH） R（ R^1 = H or methyl）, that is, the proton chemical shift of the α-R^1 group at the hydroxvl group in the all-isomer, in general, aPPears in a higher field with ca.展开更多
基金supported by Grants from the National Key R&D Programme of China (2017YFD051105)the National Natural Science Foundation of China (31630079)+2 种基金the National Science and Technology Major Project (2018ZX10101004)the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB29010000)supported by Youth Innovation Promotion Association of CAS (2019)
文摘Interferon, a natural protein that is produced by a variety of cells during viral infection, activates the transcription of multiple functional genes in cells, regulates synergy among various signaling pathways, and mediates many biological functions such as antiviral activity, immune regulation, and cell growth. However, clinical research on interferon in livestock is lacking. In this study, recombinant porcine interferon(PoIFNa) was used as an adjuvant, in combination with inactivated influenza virus, to vaccinate 6-week-old pigs via nasal infusion. The transcription of target genes was then monitored and the functions of PoIFNa were determined with respect to the activation of mucosal immunity. We found that a combination of low-dose PoIFNa and inactivated influenza virus could significantly up-regulate the expression of immunoregulatory cytokines such as IL-2, IL-18, IFN-c, IL-6, and IL-10 by real-time PCR, suggesting the induction of a strong mucosal innate immune response after administration. In addition, low-dose PoIFNa can significant enhancing the transcription of genes encoding homing factors including CCR9 and CCR10(P \ 0.001), thereby resulting in the induction of higher levels of HA-specific antibodies(P \ 0.05), which can be determined by ELISA and IFA. Post-immunization challenges with H1 N1 virus demonstrated that PoIFNa, combined with inactivated influenza virus, could alleviate clinical signs in pigs during the early stages of viral infection. These studies reveal low-dose PoIFNa as a potential mucosal adjuvant for influenza virus in pigs.
基金supported by National Natural Science Foundation of China(11871452,12071473)the Beijing Information Science and Technology University Foundation(2025031)。
文摘In this paper,we study a boundedness property of the Adams type for multilinear fractional integral operators with the multilinear L^(r′,α)-Hörmander condition and their commutators with vector valued BMO functions on a Morrey space and a predual Morrey space.Moreover,we give an endpoint estimate for multilinear fractional integral operators.As an application,we obtain the boundedness of multilinear Fourier multipliers with limited Sobolev regularity on a Morrey space.
文摘There is a classic experiential rule about 1,2-diol RCR^1 （OH） CR^1 （OH） R（ R^1 = H or methyl）, that is, the proton chemical shift of the α-R^1 group at the hydroxvl group in the all-isomer, in general, aPPears in a higher field with ca.
