目的观察曲安奈德联合A型肉毒毒素(botulinum toxin type A, BTX-A)注射治疗后行SRT-100 X线照射治疗瘢痕疙瘩的长期疗效。方法将自2016年9月至2017年9月收治的30例瘢痕疙瘩患者随机分为A组(15例)和B组(15例)。A组于瘢痕疙瘩内注射曲安...目的观察曲安奈德联合A型肉毒毒素(botulinum toxin type A, BTX-A)注射治疗后行SRT-100 X线照射治疗瘢痕疙瘩的长期疗效。方法将自2016年9月至2017年9月收治的30例瘢痕疙瘩患者随机分为A组(15例)和B组(15例)。A组于瘢痕疙瘩内注射曲安奈德,每4周1次,共5次;B组于瘢痕疙瘩内注射曲安奈德后30 min在瘢痕疙瘩基底部注射BTX-A,2.5 U/点,每点间隔1.0 cm,每4周1次,共5次。两组在注射治疗后均采用SRT-100 X线照射,4 Gy/次,共5次,采用温哥华瘢痕评分量表与可视对比评分量表对两组治疗前后瘢痕疙瘩的整体情况进行评分,并比较两组患者治疗前后总评分差值以及治疗后的显效程度。结果两组患者治疗后的效果均较满意。B组总体显效程度高于A组(z=-2.49,P=0.01)。A组和B组患者治疗前后总评分差值分别为(5.13±1.36)分、(8.67±1.05)分,两组比较其差异具有统计学意义(t=7.99,P<0.05)。结论采用曲安奈德联合BTX-A注射后行SRT-100 X线照射治疗是预防瘢痕疙瘩复发的有效措施,值得临床推广应用。展开更多
Objective To observe the effects of signal factors of corticosterone （CS）, cAMP, cGMP, Ca^2＋ and protein kinase C （PKC） on lymphocyte apoptosis in mouse thymus induced by X-rays of 4 Gy in vitro. Methods The DNA ...Objective To observe the effects of signal factors of corticosterone （CS）, cAMP, cGMP, Ca^2＋ and protein kinase C （PKC） on lymphocyte apoptosis in mouse thymus induced by X-rays of 4 Gy in vitro. Methods The DNA lyric rate for thymocytes was measured by fluomspectrophotometry. Results The DNA lyric rate for thymocytes 4-8 hours after irradiation with 2-8 Gy was significantly higher than that in the control （P〈0.01）. As compared with the control, the DNA lyric rate for thymocytes treated with 0.01 μnol/L CS （P〈0.01）, 50 ng/mL cAMP （P〈0.01）, 0.05-0.4 μg/mL ionomycin （Iono, P〈0.05 or P〈0.01） or 0.05-0.4 ng/mL phorbol myristate acetate （PMA, P〈0.05 or P〈0.01）, respectively, was significantly increased, while the rate for thymocytes treated with 50 ng/mL cGMP was not significantly increased. The DNA lyric rate for thymocytes treated with 0.01 μmol/L CS （P〈0.01）, 50 ng/mL cAMP （P〈0.01）, 0.2 and 0.4 μg/mL Iono （P〈0.05）, and 0.2 and 0.4 ng/mL PMA （P〈0.05） plus 4-Gy irradiation, respectively, was significantly higher than that treated with single 4-Gy irradiation, while the rate for thymocytes treated with 50 ng/mL cGMP plus 4-Gy irradiation was not increased. When both 0.4 I.tg/mL Iono and 0.4 ng/mL PMA acted on the thymocytes, the DNA lyric rate for thymocytes was significantly higher than that in the control （P〈0.01）, the DNA lytic rate for thymocytes treated with both 0.4 μg/mL Iono and 0.4 ng/mL PMA plus 4-Gy irradiation was significantly higher than that treated with single 4-Gy irradiation （P〈0.05）, but was Iono plus 4-Gy irradiation or 0.4 ng/mL PMA plus 4-Gy irradiation. can promote thymocyte apoptosis induced by larger dose X-rays. not significantly higher than that treated with 0.4 μg/mL Conclusion CS, cAMP, Ca^2＋, and PKC signal factors can promote thymocyte apoptosis induced by larger dose X-rays.展开更多
Long persistent phosphors have received significant attention owing to their attractive photophysical properties.Here,we report a new long persistent phosphor exhibiting strong ultraviolet A(UVA)afterglow.The phosphor...Long persistent phosphors have received significant attention owing to their attractive photophysical properties.Here,we report a new long persistent phosphor exhibiting strong ultraviolet A(UVA)afterglow.The phosphors were synthesized by a solid-state reaction method.We find that the obtained phosphors demonstrate super long UVA-afterglow emissions after irradiation by X-ray source,and the afterglow can last more than 50 h.A wide range of experimental characterizations indicate that the Tb^3+doped fluoride elpasolite phosphors are defective and some fluoride ions are replaced by oxygen ions,which creates electron traps with suitable trap depths.Our results establish that Tb^3+can act as optical emitters in wide-bandgap hosts that can result in the UVA afterglow.This work enriches the bank of UV long persistent phosphors,and may stimulate more efforts for the design and synthesis of this kind of optical materials.展开更多
X-ray excited photodynamic therapy(X-PDT)is the bravo answer of photodynamic therapy(PDT)for deep-seated tumors,as it employs X-ray as the irradiation source to overcome the limitation of light penetration depth.Howev...X-ray excited photodynamic therapy(X-PDT)is the bravo answer of photodynamic therapy(PDT)for deep-seated tumors,as it employs X-ray as the irradiation source to overcome the limitation of light penetration depth.However,high X-ray irradiation dose caused organ lesions and side effects became the major barrier to X-PDT application.To address this issue,this work employed a classic-al co-precipitation reaction to synthesize NaLuF_(4):15%Tb^(3+)(NLF)with an average particle size of(23.48±0.91)nm,which was then coupled with the photosensitizer merocyanine 540(MC540)to form the X-PDT system NLF-MC540 with high production of singlet oxygen.The system could induce antitumor efficacy to about 24%in relative low dose X-ray irradiation range(0.1-0.3 Gy).In vivo,when NLF-MC540 irradiated by 0.1 Gy X-ray,the tumor inhibition percentage reached 89.5%±5.7%.The therapeutic mechanism of low dose X-PDT was found.A significant increase of neutrophils in serum was found on the third day after X-PDT.By immunohistochemical staining of tumor sections,the Ly6G^(+),CD8^(+),and CD11c^(+)cells infiltrated in the tumor microenvironment were studied.Utilizing the bilat-eral tumor model,the NLF-MC540 with 0.1 Gy X-ray irradiation could inhibit both the primary tumor and the distant tumor growth.De-tected by enzyme linked immunosorbent assay(ELISA),two cytokines IFN-γand TNF-αin serum were upregulated 7 and 6 times than negative control,respectively.Detected by enzyme linked immune spot assay(ELISPOT),the number of immune cells attributable to the IFN-γand TNF-αlevels in the group of low dose X-PDT were 14 and 6 times greater than that in the negative control group,respectively.Thus,it conclude that low dose X-PDT system could successfully upregulate the levels of immune cells,stimulate the secretion of cy-tokines(especially IFN-γand TNF-α),activate antitumor immunity,and finally inhibit colon tumor growth.展开更多
基金This study was supported by a grant from the National Natural Science Foundation of China (No. 391702750)
文摘Objective To observe the effects of signal factors of corticosterone （CS）, cAMP, cGMP, Ca^2＋ and protein kinase C （PKC） on lymphocyte apoptosis in mouse thymus induced by X-rays of 4 Gy in vitro. Methods The DNA lyric rate for thymocytes was measured by fluomspectrophotometry. Results The DNA lyric rate for thymocytes 4-8 hours after irradiation with 2-8 Gy was significantly higher than that in the control （P〈0.01）. As compared with the control, the DNA lyric rate for thymocytes treated with 0.01 μnol/L CS （P〈0.01）, 50 ng/mL cAMP （P〈0.01）, 0.05-0.4 μg/mL ionomycin （Iono, P〈0.05 or P〈0.01） or 0.05-0.4 ng/mL phorbol myristate acetate （PMA, P〈0.05 or P〈0.01）, respectively, was significantly increased, while the rate for thymocytes treated with 50 ng/mL cGMP was not significantly increased. The DNA lyric rate for thymocytes treated with 0.01 μmol/L CS （P〈0.01）, 50 ng/mL cAMP （P〈0.01）, 0.2 and 0.4 μg/mL Iono （P〈0.05）, and 0.2 and 0.4 ng/mL PMA （P〈0.05） plus 4-Gy irradiation, respectively, was significantly higher than that treated with single 4-Gy irradiation, while the rate for thymocytes treated with 50 ng/mL cGMP plus 4-Gy irradiation was not increased. When both 0.4 I.tg/mL Iono and 0.4 ng/mL PMA acted on the thymocytes, the DNA lyric rate for thymocytes was significantly higher than that in the control （P〈0.01）, the DNA lytic rate for thymocytes treated with both 0.4 μg/mL Iono and 0.4 ng/mL PMA plus 4-Gy irradiation was significantly higher than that treated with single 4-Gy irradiation （P〈0.05）, but was Iono plus 4-Gy irradiation or 0.4 ng/mL PMA plus 4-Gy irradiation. can promote thymocyte apoptosis induced by larger dose X-rays. not significantly higher than that treated with 0.4 μg/mL Conclusion CS, cAMP, Ca^2＋, and PKC signal factors can promote thymocyte apoptosis induced by larger dose X-rays.
基金supported by the National Natural Science Foundation of China(11574225,11874275)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)(61564007).
文摘Long persistent phosphors have received significant attention owing to their attractive photophysical properties.Here,we report a new long persistent phosphor exhibiting strong ultraviolet A(UVA)afterglow.The phosphors were synthesized by a solid-state reaction method.We find that the obtained phosphors demonstrate super long UVA-afterglow emissions after irradiation by X-ray source,and the afterglow can last more than 50 h.A wide range of experimental characterizations indicate that the Tb^3+doped fluoride elpasolite phosphors are defective and some fluoride ions are replaced by oxygen ions,which creates electron traps with suitable trap depths.Our results establish that Tb^3+can act as optical emitters in wide-bandgap hosts that can result in the UVA afterglow.This work enriches the bank of UV long persistent phosphors,and may stimulate more efforts for the design and synthesis of this kind of optical materials.
基金funded by the National Natural Science Foundation of China (Nos.81771972,52171243,and 52371256)the National Key Research and Development Program of China (No.2017YFC0107405).
文摘X-ray excited photodynamic therapy(X-PDT)is the bravo answer of photodynamic therapy(PDT)for deep-seated tumors,as it employs X-ray as the irradiation source to overcome the limitation of light penetration depth.However,high X-ray irradiation dose caused organ lesions and side effects became the major barrier to X-PDT application.To address this issue,this work employed a classic-al co-precipitation reaction to synthesize NaLuF_(4):15%Tb^(3+)(NLF)with an average particle size of(23.48±0.91)nm,which was then coupled with the photosensitizer merocyanine 540(MC540)to form the X-PDT system NLF-MC540 with high production of singlet oxygen.The system could induce antitumor efficacy to about 24%in relative low dose X-ray irradiation range(0.1-0.3 Gy).In vivo,when NLF-MC540 irradiated by 0.1 Gy X-ray,the tumor inhibition percentage reached 89.5%±5.7%.The therapeutic mechanism of low dose X-PDT was found.A significant increase of neutrophils in serum was found on the third day after X-PDT.By immunohistochemical staining of tumor sections,the Ly6G^(+),CD8^(+),and CD11c^(+)cells infiltrated in the tumor microenvironment were studied.Utilizing the bilat-eral tumor model,the NLF-MC540 with 0.1 Gy X-ray irradiation could inhibit both the primary tumor and the distant tumor growth.De-tected by enzyme linked immunosorbent assay(ELISA),two cytokines IFN-γand TNF-αin serum were upregulated 7 and 6 times than negative control,respectively.Detected by enzyme linked immune spot assay(ELISPOT),the number of immune cells attributable to the IFN-γand TNF-αlevels in the group of low dose X-PDT were 14 and 6 times greater than that in the negative control group,respectively.Thus,it conclude that low dose X-PDT system could successfully upregulate the levels of immune cells,stimulate the secretion of cy-tokines(especially IFN-γand TNF-α),activate antitumor immunity,and finally inhibit colon tumor growth.
