摘要
用 Ｌ９２９细胞染料摄入法检测了小鼠受不同剂量（７５ｍＧｙ； ２．０Ｇｙ）Ｘ射线全身照射后小鼠腹腔巨噬细胞肿瘤坏死因子α（ＴＮＦα）表达量的时程变化及全身照射后 ２４ｈ的剂量一效应关系。结果可见， ７５ｍＧｙ照射后 ３ｈ， ＴＮＦα表达量有所增加，从 ６～１２０ｈ，均显著高于对照组，１２ｈ表达量最高；２．０Ｇｙ照射后３～１２０ｈ，不同时间点均显著高于对照，表达量最高点为１２ｈ。从剂量一效应关系中可见，经 ５０ｍＧｙ～２．０Ｇｙ不同剂量照射后， ＴＮＦα表达量均显著高于对照组， ０．５Ｇｙ照射组其表达量最高，而经 ４．０Ｇｙ和 ６．０Ｇｙ照射后， ＴＮＦα的表达量显著低于对照组。提示一定剂量电离辐射可诱导巨噬细胞产生ＴＮＦα。
The TNF-mediated L929 cell toxicity bioassay was used to examine the expression of TNFα in mouse peritoneal macrophages following whole body irradiation(WBI). The wxpression of TNFα gradually increased after WBI with 0.075Gy, reaching its peak at 12h. The TNFα expression of TNFα gradually increassed from 3 to 120h after WBI with 2Gy . The expression of TNFα was significantly increased at 24h after WBI with 0.05, 0.075 , 0.1,0.2,0.5, 1.0 and 2.0Gy . The expression of TNFα increased most markedly in the group irradiated with 0.5Gy.
出处
《辐射研究与辐射工艺学报》
CSCD
北大核心
2000年第3期236-239,共4页
Journal of Radiation Research and Radiation Processing
基金
国家自然科学基金资助!(39570188)
关键词
X线辐射
腹腔巨噬细胞
肿瘤坏死因子Α
X-ray irradiation
Peritoneal macrophages
Tumor necrosis facforα
