AIM: To explore the anti-hepatitis B virus (HBV) effects of Boehmeria nivea (B. nivea) root extract (BNE) by using the HepG2 2.2.15 cell model system. METHODS: Hepatitis B surface antigen (HBsAg), hepatitis B virus e ...AIM: To explore the anti-hepatitis B virus (HBV) effects of Boehmeria nivea (B. nivea) root extract (BNE) by using the HepG2 2.2.15 cell model system. METHODS: Hepatitis B surface antigen (HBsAg), hepatitis B virus e antigen (HBeAg), and HBV DNA were measured by using ELISA and real-time PCR, respectively. Viral DNA replication and RNA expression were determined by using Southern and Northern blot, respectively. RESULTS: In HepG2 2.2.15 cells, HBeAg (60%, P < 0.01) and particle-associated HBV DNA (> 99%, P < 0.01) secretion into supernatant were significantly inhibited by BNE at a dose of 100 mg/L, whereas the HBsAg was not inhibited. With different doses of BNE, the reduced HBeAg was correlated with the inhibition of HBV DNA. The anti-HBV effect of BNE was not caused by its cytotoxicity to cells or inhibition of viral DNA replication and RNA expression. CONCLUSION: BNE could effectively reduce the HBV production and its anti-HBV machinery might differ from the nucleoside analogues.展开更多
A new quinolizidine alkaloid. 3-(4-hydroxyphenyl)-4-(3-methoxy-4-hydroxyphenyl)-3,4-dihydroquinolizidine (1). was isolated from the ethanol extract of the whole plants of Boehmeria siamensis Craib. Its structure was e...A new quinolizidine alkaloid. 3-(4-hydroxyphenyl)-4-(3-methoxy-4-hydroxyphenyl)-3,4-dihydroquinolizidine (1). was isolated from the ethanol extract of the whole plants of Boehmeria siamensis Craib. Its structure was elucidated on the analysis of ID NMR and 2D NMR spectrum.展开更多
Boehmeria nivea var.strigosa Zeng Y.Wu&Y.Zhao,a new variety of B.nivea(Urticaceae)from Southwest China,is here described based on evidence from morphology and molecular phylogeny.This new variety is mainly charact...Boehmeria nivea var.strigosa Zeng Y.Wu&Y.Zhao,a new variety of B.nivea(Urticaceae)from Southwest China,is here described based on evidence from morphology and molecular phylogeny.This new variety is mainly characterized by its green abaxial leaf blade,partly connate stipules,and densely patent strigose hairs on stems and potioles.The phylogenetic analysis based on rbc L,nrDNA and rbc L+nrDNA datasets,revealed that all individuals of B.nivea var.strigosa formed a monophyletic group.The conservation status of B.nivea var.strigosa is assessed as“Near Threatened”(NT)according to IUCN evaluation criteria.The discovery of this new variety is not only crucial for the taxonomy of ramie,but also provides reference for the exploration and utilization of ramie.展开更多
To explore the role of ethylene in sexual determination in ramie, the ethylene release rates in ramie stem apex of different sex, bud of the same node in female and hermaphrodite ramie in the second crop, and single i...To explore the role of ethylene in sexual determination in ramie, the ethylene release rates in ramie stem apex of different sex, bud of the same node in female and hermaphrodite ramie in the second crop, and single inflorescence of different sex in hermaphrodite ramie were measured by gas chromatography. Effects of two ethylene inhibitors on sex expression in ramie were investigated. The ethylene release rate of stem apex was higher in the second crop or female ramie than that in the third crop or hermaphrodite ramie during growth. Although ethylene release rates between lower nodes and higher nodes were little different, it was lower in the middle nodes in hermaphrodite ramie. The ethylene release rates were higher in the higher nodes of female ramie at the second crop. At the third crop, the ethylene release rates were higher in lower nodes, lower in middle nodes and then highest in higher nodes in female ramie. However, an opposite ethylene release pattern was observed in hermaphrodite ramie. The ethylene release rate was higher in female flower than male flower and mixed inflorescence in hermaphrodite ramie. The male flower could be distinctly induced by AVG （aminooethoxyvinylglycine）. The node of the first male flower, percentage of female flowers and ratio of female flowers to male in ramie were evidently depressed by AVG in contrast to water. The percentage of mixed male and female flowers was also increased and the percentage of female flower decreased by spraying AgNO3. There was a close relationship between sexual differentiation and ethylene release rate in ramie. The female ramie could be induced by high ethylene release rate. The female flower could be inhibited by AVG and AgNO3. AVG at a concentration of 300 mg L^-1 was most effective.展开更多
By using cpSSR （chloroplast microsatellites） markers, the genetic relationship among Boehmeria varieties was analyzed. Five pairs of cpSSR primers with excellent amplification result, clear amplification band and go...By using cpSSR （chloroplast microsatellites） markers, the genetic relationship among Boehmeria varieties was analyzed. Five pairs of cpSSR primers with excellent amplification result, clear amplification band and good repeatability were screened from 22 pairs of cpSSR primers, and the polymorphism rate was 22.73%. By using these five pairs of cpSSR primers, 16 polymorphic loci were amplified from eight experimental materials, with an average of 3.2 loci. According to the results of duster analysis, these experimental materials were divided into three categories. The clustering results were consistent with the classification results based on chloroplast gene sequences.展开更多
Different ploidy ramie materials were studied via microscopic observation on stem sections and macerated fiber cells. Morphologic differences between different ploidy plants were analyzed. Average bark area percentage...Different ploidy ramie materials were studied via microscopic observation on stem sections and macerated fiber cells. Morphologic differences between different ploidy plants were analyzed. Average bark area percentages in ramie stem transection of haploids, tetraploids, diploids and triploids were 28. 91, 27. 05, 26. 97 and 24.77% respectively. Average percentages of fiber layer area of diploids, haploids, tetraploids and triploids were 16.80, 16. 58, 15.52 and 13.78% respectively. Average fiber cell diameter and cell wall thickness were increased along with the increase of the ploidy of the plants. Average fiber length of diploids, triploids and haploids were 8. 49, 7. 96 and 6. 93 cm respectively. Average L/B (length/breadth) of diploids, triploids and haploids were 2 470. 7, 2 390. 6 and 1 616.3 respectively. Average breadths of fiber of haploids, diploids and triploids were 29. 30, 33. 87 and 49. 20μm respectively. However, there were relatively large variations in the above characteristics among the ramie materials of the same ploidy levels. Field performance of different ploidy plants was also investigated. As the chromosome ploidy increasing, there was a tendency of declining in shoot number per plant and increasing in stem diameter. Average shoots per plant of haploids, diploids, triploids and tetraploids were 5.83, 5.30, 3.77 and 3. 65 whereas their average stem diameters were 0.66, 0. 67, 0. 74 and 0. 76 cm respectively. Triploids were the tallest, while haploids were the shortest. Triploids had strong growth vigour, diploids and tetraploids had moderate growth vigour, while haploids appeared to be lack of growth vigour. Cold stress tolerance of tetraploids were the strongest, diploid had the moderate tolerance, while haploids and triploids were the least tolerant to cold stress. Both haploids and triploids were sterile.展开更多
Six male sterile lines (MSLs) of ramie (Boeh-meria nivea (L.) Gaud.) were grown in dark rooms under the photoperiods of 9.5h, 11h or 12.5h d-1 in spring and summer seasons (dif-ferent environmental temperatures) to te...Six male sterile lines (MSLs) of ramie (Boeh-meria nivea (L.) Gaud.) were grown in dark rooms under the photoperiods of 9.5h, 11h or 12.5h d-1 in spring and summer seasons (dif-ferent environmental temperatures) to test their developmental response to photoperiod and temperature. The MSLs showed little difference in vegetative growth duration, but different de-velopment rates in the reproductive growth stage under the tested conditions. Higher tem-perature (grown in summer) mainly accelerated vegetative growth, while the short photoperiod treatment accelerated the reproductive growth of the MSLs. Moreover, the short photoperiod treatment combined with higher temperature obviously accelerated both the vegetative and reproductive growth of the MSLs. But the effect of higher temperature decreased, or even dis-appeared along with the photoperiod elongation. The MSLs were divided into 5 photo-temperature response types, based on the flower budding acceleration of short pho-toperiod and the approximate temperature re-sponse index.展开更多
文摘AIM: To explore the anti-hepatitis B virus (HBV) effects of Boehmeria nivea (B. nivea) root extract (BNE) by using the HepG2 2.2.15 cell model system. METHODS: Hepatitis B surface antigen (HBsAg), hepatitis B virus e antigen (HBeAg), and HBV DNA were measured by using ELISA and real-time PCR, respectively. Viral DNA replication and RNA expression were determined by using Southern and Northern blot, respectively. RESULTS: In HepG2 2.2.15 cells, HBeAg (60%, P < 0.01) and particle-associated HBV DNA (> 99%, P < 0.01) secretion into supernatant were significantly inhibited by BNE at a dose of 100 mg/L, whereas the HBsAg was not inhibited. With different doses of BNE, the reduced HBeAg was correlated with the inhibition of HBV DNA. The anti-HBV effect of BNE was not caused by its cytotoxicity to cells or inhibition of viral DNA replication and RNA expression. CONCLUSION: BNE could effectively reduce the HBV production and its anti-HBV machinery might differ from the nucleoside analogues.
文摘A new quinolizidine alkaloid. 3-(4-hydroxyphenyl)-4-(3-methoxy-4-hydroxyphenyl)-3,4-dihydroquinolizidine (1). was isolated from the ethanol extract of the whole plants of Boehmeria siamensis Craib. Its structure was elucidated on the analysis of ID NMR and 2D NMR spectrum.
文摘Boehmeria nivea var.strigosa Zeng Y.Wu&Y.Zhao,a new variety of B.nivea(Urticaceae)from Southwest China,is here described based on evidence from morphology and molecular phylogeny.This new variety is mainly characterized by its green abaxial leaf blade,partly connate stipules,and densely patent strigose hairs on stems and potioles.The phylogenetic analysis based on rbc L,nrDNA and rbc L+nrDNA datasets,revealed that all individuals of B.nivea var.strigosa formed a monophyletic group.The conservation status of B.nivea var.strigosa is assessed as“Near Threatened”(NT)according to IUCN evaluation criteria.The discovery of this new variety is not only crucial for the taxonomy of ramie,but also provides reference for the exploration and utilization of ramie.
基金supported by the National Natural Science Foundation of China (30270849)
文摘To explore the role of ethylene in sexual determination in ramie, the ethylene release rates in ramie stem apex of different sex, bud of the same node in female and hermaphrodite ramie in the second crop, and single inflorescence of different sex in hermaphrodite ramie were measured by gas chromatography. Effects of two ethylene inhibitors on sex expression in ramie were investigated. The ethylene release rate of stem apex was higher in the second crop or female ramie than that in the third crop or hermaphrodite ramie during growth. Although ethylene release rates between lower nodes and higher nodes were little different, it was lower in the middle nodes in hermaphrodite ramie. The ethylene release rates were higher in the higher nodes of female ramie at the second crop. At the third crop, the ethylene release rates were higher in lower nodes, lower in middle nodes and then highest in higher nodes in female ramie. However, an opposite ethylene release pattern was observed in hermaphrodite ramie. The ethylene release rate was higher in female flower than male flower and mixed inflorescence in hermaphrodite ramie. The male flower could be distinctly induced by AVG （aminooethoxyvinylglycine）. The node of the first male flower, percentage of female flowers and ratio of female flowers to male in ramie were evidently depressed by AVG in contrast to water. The percentage of mixed male and female flowers was also increased and the percentage of female flower decreased by spraying AgNO3. There was a close relationship between sexual differentiation and ethylene release rate in ramie. The female ramie could be induced by high ethylene release rate. The female flower could be inhibited by AVG and AgNO3. AVG at a concentration of 300 mg L^-1 was most effective.
基金Supported by Fund for Basic Scientific Research and Operating Expenses of Chinese Academy Of Agricultural Sciences(1610032012030)
文摘By using cpSSR （chloroplast microsatellites） markers, the genetic relationship among Boehmeria varieties was analyzed. Five pairs of cpSSR primers with excellent amplification result, clear amplification band and good repeatability were screened from 22 pairs of cpSSR primers, and the polymorphism rate was 22.73%. By using these five pairs of cpSSR primers, 16 polymorphic loci were amplified from eight experimental materials, with an average of 3.2 loci. According to the results of duster analysis, these experimental materials were divided into three categories. The clustering results were consistent with the classification results based on chloroplast gene sequences.
文摘Different ploidy ramie materials were studied via microscopic observation on stem sections and macerated fiber cells. Morphologic differences between different ploidy plants were analyzed. Average bark area percentages in ramie stem transection of haploids, tetraploids, diploids and triploids were 28. 91, 27. 05, 26. 97 and 24.77% respectively. Average percentages of fiber layer area of diploids, haploids, tetraploids and triploids were 16.80, 16. 58, 15.52 and 13.78% respectively. Average fiber cell diameter and cell wall thickness were increased along with the increase of the ploidy of the plants. Average fiber length of diploids, triploids and haploids were 8. 49, 7. 96 and 6. 93 cm respectively. Average L/B (length/breadth) of diploids, triploids and haploids were 2 470. 7, 2 390. 6 and 1 616.3 respectively. Average breadths of fiber of haploids, diploids and triploids were 29. 30, 33. 87 and 49. 20μm respectively. However, there were relatively large variations in the above characteristics among the ramie materials of the same ploidy levels. Field performance of different ploidy plants was also investigated. As the chromosome ploidy increasing, there was a tendency of declining in shoot number per plant and increasing in stem diameter. Average shoots per plant of haploids, diploids, triploids and tetraploids were 5.83, 5.30, 3.77 and 3. 65 whereas their average stem diameters were 0.66, 0. 67, 0. 74 and 0. 76 cm respectively. Triploids were the tallest, while haploids were the shortest. Triploids had strong growth vigour, diploids and tetraploids had moderate growth vigour, while haploids appeared to be lack of growth vigour. Cold stress tolerance of tetraploids were the strongest, diploid had the moderate tolerance, while haploids and triploids were the least tolerant to cold stress. Both haploids and triploids were sterile.
文摘Six male sterile lines (MSLs) of ramie (Boeh-meria nivea (L.) Gaud.) were grown in dark rooms under the photoperiods of 9.5h, 11h or 12.5h d-1 in spring and summer seasons (dif-ferent environmental temperatures) to test their developmental response to photoperiod and temperature. The MSLs showed little difference in vegetative growth duration, but different de-velopment rates in the reproductive growth stage under the tested conditions. Higher tem-perature (grown in summer) mainly accelerated vegetative growth, while the short photoperiod treatment accelerated the reproductive growth of the MSLs. Moreover, the short photoperiod treatment combined with higher temperature obviously accelerated both the vegetative and reproductive growth of the MSLs. But the effect of higher temperature decreased, or even dis-appeared along with the photoperiod elongation. The MSLs were divided into 5 photo-temperature response types, based on the flower budding acceleration of short pho-toperiod and the approximate temperature re-sponse index.
