Enzyme-based anticancer therapy is more attractive for the less side effect than conventional chemotherapy.However,the poor stability and low membrane permeability of enzymes during the intracellular delivery are cons...Enzyme-based anticancer therapy is more attractive for the less side effect than conventional chemotherapy.However,the poor stability and low membrane permeability of enzymes during the intracellular delivery are constraints for its practical applications.In this work,we synthesized novel near-infrared (NIR)-responsive core-shell-structured Prussian blue@fibrous SiO2 (PBFS) nanoparticles as the carrier of superoxide dismutase (SOD) and a glutathione (GSH)-activated Fenton reagent (DiFe).The PBFS nanoparticles are further modified with aGSH-responsive cationic polymer (poly(2-(acryloyloxy)-N,N-dimethyl-N-(4-(((2-((2-(((4-methyl-2-oxo-2H-chromen-7-yl)carbamoyl)oxy)ethyl)disulfaneyl)ethoxy)carbonyl)amino)benzyl)ethan-1-aminium,PSS) containing disulfide bonds and fluorophores.After SOD and DiFe are loaded on the PBFS-PSS nanoparticles,dual chemodynamic/photothermal therapeutic nanoparticulate systems (PBFS-PSS/DiFe/SOD) are obtained.In vitro experiments show that PBFS-PSS/DiFe/SOD nanoparticles have good biocompatibility and can be tracked under fluorescence microscope during the intracellular delivery process in MCF-7 tumor cells due to the GSH-activated release of fluorophores.They also exhibit high efficiency in NIR photothermal conversion and GSH-activated Fenton reaction in tumor cells,thus achieving high-efficient killing effect of tumor cells based on the combination of photothermal and chemodynamic therapeutic performance (PTT and CDT).This work offers a novel pathway to construct a visual multifunctional nanomedicine platform for future cancer therapy.展开更多
An efficient synthesis of various a-halo,a-allylic aldehydes from a,a-dihalo ketones using both cyclic （3-bromocyclohex-1-ene zinc bromide and （Z）-3-bromocyelobut-l-ene zinc bromide） and acyclic （allylzinc bromid...An efficient synthesis of various a-halo,a-allylic aldehydes from a,a-dihalo ketones using both cyclic （3-bromocyclohex-1-ene zinc bromide and （Z）-3-bromocyelobut-l-ene zinc bromide） and acyclic （allylzinc bromide and cinnamylzinc bromide） type of allylic organozinc bromide with DMF as base is described. A possible reaction mechanism is also proposed.展开更多
基金the National Natural Science Foundation of China (Nos.51473152 and 51573174).
文摘Enzyme-based anticancer therapy is more attractive for the less side effect than conventional chemotherapy.However,the poor stability and low membrane permeability of enzymes during the intracellular delivery are constraints for its practical applications.In this work,we synthesized novel near-infrared (NIR)-responsive core-shell-structured Prussian blue@fibrous SiO2 (PBFS) nanoparticles as the carrier of superoxide dismutase (SOD) and a glutathione (GSH)-activated Fenton reagent (DiFe).The PBFS nanoparticles are further modified with aGSH-responsive cationic polymer (poly(2-(acryloyloxy)-N,N-dimethyl-N-(4-(((2-((2-(((4-methyl-2-oxo-2H-chromen-7-yl)carbamoyl)oxy)ethyl)disulfaneyl)ethoxy)carbonyl)amino)benzyl)ethan-1-aminium,PSS) containing disulfide bonds and fluorophores.After SOD and DiFe are loaded on the PBFS-PSS nanoparticles,dual chemodynamic/photothermal therapeutic nanoparticulate systems (PBFS-PSS/DiFe/SOD) are obtained.In vitro experiments show that PBFS-PSS/DiFe/SOD nanoparticles have good biocompatibility and can be tracked under fluorescence microscope during the intracellular delivery process in MCF-7 tumor cells due to the GSH-activated release of fluorophores.They also exhibit high efficiency in NIR photothermal conversion and GSH-activated Fenton reaction in tumor cells,thus achieving high-efficient killing effect of tumor cells based on the combination of photothermal and chemodynamic therapeutic performance (PTT and CDT).This work offers a novel pathway to construct a visual multifunctional nanomedicine platform for future cancer therapy.
基金This work was supported by the National Natural Science Foundation of China （No. 21072143）.
文摘An efficient synthesis of various a-halo,a-allylic aldehydes from a,a-dihalo ketones using both cyclic （3-bromocyclohex-1-ene zinc bromide and （Z）-3-bromocyelobut-l-ene zinc bromide） and acyclic （allylzinc bromide and cinnamylzinc bromide） type of allylic organozinc bromide with DMF as base is described. A possible reaction mechanism is also proposed.
基金Project supported by the National Natural Science Foundation of China （No. 20572124）.
文摘Alkyl sulfoxides were synthesized in good yields from the reaction of alkanesulfinyl chloride with Grignard reagents in the presence of zinc chloride.
