AIM: To determine the expression of PGH synthase-1and the sensitivity of vascular smooth muscle to PGH_2in the aorta from the SHR at an age when noendothelium-dependent contractions to acetylcholine areobserved under ...AIM: To determine the expression of PGH synthase-1and the sensitivity of vascular smooth muscle to PGH_2in the aorta from the SHR at an age when noendothelium-dependent contractions to acetylcholine areobserved under control conditions. METHODS: Allexperiments were performed in parallel on aortas from20-wk-old SHR and Wistar-Kyoto normotensive rats(WKY). Rings, with or without endothelium, weresuspended in conventional organ chambers for therecording of changes in isometric force. Theexpression of PGH Synthase-1 was evaluated by展开更多
目的探讨高迁移率蛋白B1(HMGB1)、基质金属蛋白酶-9(MMP-9)m RNA和蛋白在子宫内膜癌组织中表达水平及临床意义。方法应用实时荧光定量RT-PCR及Western印记法检测正常子宫内膜组织、单纯型增生症子宫内膜组织、非典型增生子宫内膜组织和...目的探讨高迁移率蛋白B1(HMGB1)、基质金属蛋白酶-9(MMP-9)m RNA和蛋白在子宫内膜癌组织中表达水平及临床意义。方法应用实时荧光定量RT-PCR及Western印记法检测正常子宫内膜组织、单纯型增生症子宫内膜组织、非典型增生子宫内膜组织和子宫内膜癌Ⅰ期、Ⅱ期及Ⅲ期组织中HMGB1、MMP-9 m RNA和蛋白的表达水平。结果与正常子宫内膜组织比较,子宫内膜癌组织HMGB1、MMP-9 m RNA及蛋白的表达量明显升高,差异有统计学意义(P<0.05);子宫内膜癌组织HMGB1、MMP-9蛋白的表达上调与手术病理分期、淋巴结转移及浸润深度有关(P<0.05),与癌组织的分化程度无关(P>0.05);子宫内膜癌组织中,HMGB1蛋白与MMP-9蛋白的表达呈正相关(r=0.900,P<0.05)。结论 HMGB1、MMP-9在子宫内膜癌的发生、发展及转移的过程中起着重要的作用,其有望成为子宫内膜癌的早期诊断及预后评价的分子标志物。展开更多
文摘AIM: To determine the expression of PGH synthase-1and the sensitivity of vascular smooth muscle to PGH_2in the aorta from the SHR at an age when noendothelium-dependent contractions to acetylcholine areobserved under control conditions. METHODS: Allexperiments were performed in parallel on aortas from20-wk-old SHR and Wistar-Kyoto normotensive rats(WKY). Rings, with or without endothelium, weresuspended in conventional organ chambers for therecording of changes in isometric force. Theexpression of PGH Synthase-1 was evaluated by
文摘目的探讨高迁移率蛋白B1(HMGB1)、基质金属蛋白酶-9(MMP-9)m RNA和蛋白在子宫内膜癌组织中表达水平及临床意义。方法应用实时荧光定量RT-PCR及Western印记法检测正常子宫内膜组织、单纯型增生症子宫内膜组织、非典型增生子宫内膜组织和子宫内膜癌Ⅰ期、Ⅱ期及Ⅲ期组织中HMGB1、MMP-9 m RNA和蛋白的表达水平。结果与正常子宫内膜组织比较,子宫内膜癌组织HMGB1、MMP-9 m RNA及蛋白的表达量明显升高,差异有统计学意义(P<0.05);子宫内膜癌组织HMGB1、MMP-9蛋白的表达上调与手术病理分期、淋巴结转移及浸润深度有关(P<0.05),与癌组织的分化程度无关(P>0.05);子宫内膜癌组织中,HMGB1蛋白与MMP-9蛋白的表达呈正相关(r=0.900,P<0.05)。结论 HMGB1、MMP-9在子宫内膜癌的发生、发展及转移的过程中起着重要的作用,其有望成为子宫内膜癌的早期诊断及预后评价的分子标志物。
