目的探讨抑制人肺癌相关抗原基因ALT04-AG表达对细胞生长特性及相关基因表达的影响。方法重组反义ALT04-AGRNA真核表达质粒,经脂质体介导转染人肺癌细胞株(L78),以MTT、FCM法分析转染细胞生长特性,以Northern b lot、免疫组化染色及基...目的探讨抑制人肺癌相关抗原基因ALT04-AG表达对细胞生长特性及相关基因表达的影响。方法重组反义ALT04-AGRNA真核表达质粒,经脂质体介导转染人肺癌细胞株(L78),以MTT、FCM法分析转染细胞生长特性,以Northern b lot、免疫组化染色及基因芯片技术检测相关基因的表达。结果构建了重组表达反义ALT04-AGRNA的真核表达质粒[pALT04-AG(as)],经该质粒转染及二氟甲基鸟氨酸(d ifluorom ethylorn ith ine,DFMO)处理的L78细胞均引起ALT04-AG表达下调及细胞的增殖抑制,后者还导致细胞凋亡比例增加。结论pALT04-AG(as)转染L78细胞或用DFMO抑制多胺生物合成,可通过对相关基因表达的调控促使L78细胞恶性表型逆转,而后者的作用更为广泛。本结果为探索肿瘤的诊断与治疗提供了有意义的线索。展开更多
Hepatocellular carcinoma(HCC) is one of the most common tumors worldwide. The survival rate after the onset of symptoms is generally less than one year for the late presentation of HCC, and reliable tools for early di...Hepatocellular carcinoma(HCC) is one of the most common tumors worldwide. The survival rate after the onset of symptoms is generally less than one year for the late presentation of HCC, and reliable tools for early diagnosis are lacking. Therefore, novel biomarkers for the early detection of HCC are urgently required. Recent studies show that the abnormal release of proteins by tumor cells can elicit humoral immune responses to self-antigens called tumor-associated antigens(TAAs). The corresponding autoantibodies can be detected before the clinical diagnosis of cancer. Therefore, there is growing interest in using serum autoantibodies as cancer biomarkers. In this review, we focus on the advances in research on autoantibodies against TAAs as serum biomarker for detection of HCC, the mechanism of the production of TAAs, and the association of autoantibodies with patients' clinical characteristics.展开更多
目的：探讨血清肿瘤相关物质（TAM）和血清前列腺特异抗原（PSA）联合检测鉴别诊断前列腺疾病及前列腺癌（PCa）的临床价值。方法 TAM 采用化学显色法定量测定，PSA 采用德国罗氏公司 Cobas e 601全自动电化学发光分析仪及配套试剂检测...目的：探讨血清肿瘤相关物质（TAM）和血清前列腺特异抗原（PSA）联合检测鉴别诊断前列腺疾病及前列腺癌（PCa）的临床价值。方法 TAM 采用化学显色法定量测定，PSA 采用德国罗氏公司 Cobas e 601全自动电化学发光分析仪及配套试剂检测。结果慢性前列腺炎组、急性前列腺炎组、前列腺增生组和前列腺癌组 TAM敏感性分别为0.62%、1.94%、1.75%和94.94%，PSA 敏感性分别为40.12%、79.61%、47.81%和96.20%，PSA ﹢TAM 两项均阳性敏感性分别为0.62%、0.97%、0.88%和93.67%，TAM 特异性分别为21.33%、23.58%、25.27%和65.89%，PSA 特异性分别为47.42%、49.38%、53.62%和78.36%，PSA ﹢ TAM 两项均阳性特异性分别为20.17%、22.29%、24.56%和98.26%，差异有统计学意义（P 〈0.01）。结论 TAM 和 PSA 联合检测在敏感性和特异性上具有很好的互补作用，对鉴别诊断前列腺疾病及前列腺癌具有重要的临床价值。展开更多
基金A grant from the National Natural Science Foundation of China,No.81071973A grant from the Scientific Research Foundation for Returned Overseas Chinese Scholars,Bureau of Human Resources and Social Security of Beijing,China(Key project,2010)
文摘Hepatocellular carcinoma(HCC) is one of the most common tumors worldwide. The survival rate after the onset of symptoms is generally less than one year for the late presentation of HCC, and reliable tools for early diagnosis are lacking. Therefore, novel biomarkers for the early detection of HCC are urgently required. Recent studies show that the abnormal release of proteins by tumor cells can elicit humoral immune responses to self-antigens called tumor-associated antigens(TAAs). The corresponding autoantibodies can be detected before the clinical diagnosis of cancer. Therefore, there is growing interest in using serum autoantibodies as cancer biomarkers. In this review, we focus on the advances in research on autoantibodies against TAAs as serum biomarker for detection of HCC, the mechanism of the production of TAAs, and the association of autoantibodies with patients' clinical characteristics.
