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G250基因沉默诱导肾癌786-0细胞凋亡

Apoptosis of renal cell carcinoma 786-0 cells induced by G250 gene silencing
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摘要 目的观察沉默G250基因对肾癌786-0细胞凋亡的影响。方法构建靶向G250的siRNA表达质粒pshRNA-G250并转染肾癌786-0细胞,TUNEL法和流式细胞仪检测G250基因沉默后肾癌786-0细胞(命名786-0/si-G250-b)的凋亡率,以转染空质粒的细胞(命名786-0/si-G250-0)为对照。结果 TUNEL法显示786-0/si-G250-b和786-0/si-G250-0细胞凋亡率分别为(21.537±3.552)%、(6.590±1.167)%,(t=6.925,P=0.002),流式细胞仪检测示细胞凋亡率分别为(33.033±2.914)%(、9.133±1.115)%,(t=13.266,P=0.000)。结论沉默G250基因的表达可诱导肾癌786-0细胞的明显凋亡。 Objective To investigate the effects of G250 gene silencing by siRNA on apoptosis of renal cell carcinoma 786-0 cell.Methods The eukaryotic expression vector named pshRNA-G250 of small interfering RNA(siRNA) targeting G250 gene was constructed and transfected into renal cell carcinoma 786-0 cells.TUNEL and flow cytometry(FCM) assay were performed to test the rate of apoptosis of 786-0 cells after G250 gene silencing(named 786-0/si-G250-b).and one that empty plasmid was transfected into(named 786-0/si-G250-0) as control group.Results TUNEL showed apoptosis rate of 786-0/si-G250-b and 786-0/si-G250-0 cell was(21.537± 3.552)%,(6.590±1.167)%,(t=6.925,P=0.002).FCM showed the rate of apoptosis was(33.033±2.914)%,(9.133±1.115)%,(t=13.266,P=0.000),respectively.Conclusion The inhibition to the expression of G250 gene can obviously induce the apoptosis of renal carcinoma 786-0 cells.
出处 《中国实验诊断学》 北大核心 2011年第4期579-582,共4页 Chinese Journal of Laboratory Diagnosis
关键词 G250基因 凋亡 肿瘤相关抗原 RNA干扰 肾细胞癌 G250 gene apoptosis tumor associate antigen RNA interference renal cell carcinoma
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  • 1Abdulrahman M,Maina N E,Morris M R,et al.Identification of novel VHL targets that are associated with the development of renal cell carcinoma[J].Oncogene,2007,26:1661. 被引量:1
  • 2Zavada J,Zavadova Z,Pastorek J,et al.Human tumor-associated cell adhesion protein MN/CA IX:identification of M75 epitope and of the region mediating cell adhesion[J].Br J Cancer,2000,82:1808. 被引量:1
  • 3Li G,Feng G,Gentil-perret A,et al.CA9 gene expression in conventional renal cell carcinoma:a potential marker for prediction of early metastasis after nephrectomy[J].Clin Exp Metastasis,2007,24(3):149. 被引量:1
  • 4Cho M,Uumura H,Kim SC,et al.Hypomethylation of the MN/CA9 promoter and upregulated MN/CA9 expression in human renal cell carcinoma[J].Br J Cancer,2001,85:563. 被引量:1
  • 5Brunelli M,Gobbo S,Cossu-rocca P,et al.Chromosomal gains in the sarcomatoid transformation of chromophobe renal cell carcinoma[J].Mod Pathol,2007,20:303. 被引量:1
  • 6陈海棠,余永伟,侯建国,常文军,余宏宇,赵晋丰,曹广文.肾细胞癌中染色体3p、9p、14q微卫星不稳定及杂合性缺失分析[J].第二军医大学学报,2008,29(6):621-625. 被引量:1
  • 7Argani P,Olgac S,Tickoo SK,et al.Xpl1 translocation renal cell carcinoma in adults:expanded clinical,pathologic,and genetic spectrum[J].Am J Surg Pathol,2007,3l:1149. 被引量:1
  • 8Osborne BA,Schwartz LM.Essential genes that regulate apoptosis[J].Trends Cell Biol,1994,4:394. 被引量:1
  • 9赵俊峰,郑少斌,姜耀东,赵善超,张香梅.G250基因siRNA表达载体的构建与鉴定[J].山东医药,2008,48(6):23-25. 被引量:5
  • 10Chen Y,Cai J,Anders MW,et al.Role of mitochondrial dysfunction in S-(1,2-dichlorovinyl)-l-cysteine-induced apoptosis[J].Toxicol Appl Pharmacol,2001,170:172. 被引量:1

二级参考文献11

  • 1M Vidaurreta,ML Maestro,S Rafael,S Veganzones,MT Sanz-Casla,J Cerdán,M Arroyo.Telomerase activity in colorectal cancer,prognostic factor and implications in the microsatellite instability pathway[J].World Journal of Gastroenterology,2007,13(28):3868-3872. 被引量:8
  • 2Jemal A, Siegel R, Ward E, et al. Cancer statistics[J]. CA Cancer J Clin,2006, 56(2) :106 - 130. 被引量:1
  • 3Oosterwijk E, Ruiter DJ, Hoedemaeker PJ, et al. Monoclonal antibody G250 recognizes a determinant present in renal cell carcinoma and absent from normal kidney[J]. Int J Cancer, 1986,38(4) :489 - 494. 被引量:1
  • 4Nakagawa Y, Uemura H, Hirao Y, et al. Radiation hybrid mapping of the human MN/CA9 locus to chromosome band 9p12-p13 [ J ]. Genomics, 1998,53 ( 1 ) : 118 - 119. 被引量:1
  • 5Pastorek J, Pastorekova S, Callebant I, et al. Cloning and characterization of MN, a human tumor-associated protein with a domain ho- mologous to carbonic anhydrase and a putative helix-loop-helix DNA binding segment[J]. Oncogene, 1994,9(10) : 2877 -2888. 被引量:1
  • 6Saarnio J, Parkkila S, Parkkila AK, et al. Immunohistochemical study of colorectal tumors for expression of a novel transmembrane carbonic anhydrase, MN/CA IX, with potential value as a marker of cell proliferation[J]. Am J P.athol, 1998, 153(1) : 279 -285. 被引量:1
  • 7Zavada J, Zavadova Z, Pastorek J, et al. Human tumor-associated cell adhension protein MN/CA IX : identification of M75 eptope and of the region mediating cell adhension [ J ]. Br J Cancer, 2000, 82 (11) : 1808 -1813. 被引量:1
  • 8Cho M, Uemura H, Kim SC, et al. Hypomethylation of the MN/CA9 promoter proximal region (SP) and upregulated MN/CA9 expression in human renal cell carcinoma[J]. Br J Cancer, 2001,85(4) : 563 - 567. 被引量:1
  • 9McKieman JM, Buttyan R, Bander NH, et al. Expression of the tumor-associated gene MN: a potential biomarker for human renal cell carcinoma[J]. Cancer Res, 1997,57(12) : 2362 -2365. 被引量:1
  • 10Bernstein E, Caudy AA, Hammond SM, et al. Role for a bidentate ribonuclease in the initiation step of RNA interference[ J]. Nature, 2001,409(6818) :363 -366. 被引量:1

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