摘要
目的观察沉默G250基因对肾癌786-0细胞凋亡的影响。方法构建靶向G250的siRNA表达质粒pshRNA-G250并转染肾癌786-0细胞,TUNEL法和流式细胞仪检测G250基因沉默后肾癌786-0细胞(命名786-0/si-G250-b)的凋亡率,以转染空质粒的细胞(命名786-0/si-G250-0)为对照。结果 TUNEL法显示786-0/si-G250-b和786-0/si-G250-0细胞凋亡率分别为(21.537±3.552)%、(6.590±1.167)%,(t=6.925,P=0.002),流式细胞仪检测示细胞凋亡率分别为(33.033±2.914)%(、9.133±1.115)%,(t=13.266,P=0.000)。结论沉默G250基因的表达可诱导肾癌786-0细胞的明显凋亡。
Objective To investigate the effects of G250 gene silencing by siRNA on apoptosis of renal cell carcinoma 786-0 cell.Methods The eukaryotic expression vector named pshRNA-G250 of small interfering RNA(siRNA) targeting G250 gene was constructed and transfected into renal cell carcinoma 786-0 cells.TUNEL and flow cytometry(FCM) assay were performed to test the rate of apoptosis of 786-0 cells after G250 gene silencing(named 786-0/si-G250-b).and one that empty plasmid was transfected into(named 786-0/si-G250-0) as control group.Results TUNEL showed apoptosis rate of 786-0/si-G250-b and 786-0/si-G250-0 cell was(21.537± 3.552)%,(6.590±1.167)%,(t=6.925,P=0.002).FCM showed the rate of apoptosis was(33.033±2.914)%,(9.133±1.115)%,(t=13.266,P=0.000),respectively.Conclusion The inhibition to the expression of G250 gene can obviously induce the apoptosis of renal carcinoma 786-0 cells.
出处
《中国实验诊断学》
北大核心
2011年第4期579-582,共4页
Chinese Journal of Laboratory Diagnosis