【目的】在德国鸢尾的组培过程中,内生菌引起的污染制约着其进行快速繁殖工厂化育苗,针对引起德国鸢尾组培苗污染的内生菌进行分离、鉴定,研究比较6种常用药剂对该菌株的抑制效果,筛选出最佳防治药剂,为德国鸢尾组培快繁过程中有效控制...【目的】在德国鸢尾的组培过程中,内生菌引起的污染制约着其进行快速繁殖工厂化育苗,针对引起德国鸢尾组培苗污染的内生菌进行分离、鉴定,研究比较6种常用药剂对该菌株的抑制效果,筛选出最佳防治药剂,为德国鸢尾组培快繁过程中有效控制内生菌污染提供科学依据。【方法】采用NA培养基分离纯化菌株(115325),通过形态特征观察、生理生化试验及16S r DNA序列同源性分析,对其进行分类鉴定。采用纸碟抑菌圈法测定6种常用药剂对供试菌株的抑制效果。【结果】供试菌株115325的形态特征,及生理生化指标与表皮葡萄球菌(Staphylococcus epidermidis)基本相同。16S r DNA序列分析表明,该菌株与解糖葡萄球菌S.saccharolyticus(L37602)进化关系最为密切,聚在同一个系统发育分支,与表皮葡萄球菌的同源性最高,为99.86%。室内药剂筛选结果表明,头孢菌素和庆大霉素对供试菌株的抑制作用最强。【结论】综合形态学特征、生理生化特性及16S r DNA序列分析结果,供试菌株115325被鉴定为表皮葡萄球菌。头孢菌素和庆大霉素可作为抑制德国鸢尾组培苗内生菌污染的首选药剂。展开更多
Colorectal cancer(CRC)is the second most common cause of cancer-related deaths in Europe and other Western countries,mainly due to the lack of wellvalidated clinically useful biomarkers with enough sensitivity and spe...Colorectal cancer(CRC)is the second most common cause of cancer-related deaths in Europe and other Western countries,mainly due to the lack of wellvalidated clinically useful biomarkers with enough sensitivity and specificity to detect this disease at early stages.Although it is well known that the pathogenesis of CRC is a progressive accumulation of mutations in multiple genes,much less is known at the proteome level.Therefore,in the last years many proteomic studies have been conducted to find new candidate protein biomarkers for diagnosis,prognosis and as therapeutic targets for this malignancy,as well as to elucidate the molecular mechanisms of colorectal carcinogenesis.An important advantage of the proteomic approaches is the capacity to look for multiple differentially expressed proteins in a single study.This review provides an overview of the recent reports describing the different proteomic tools used for the discovery of new protein markers for CRC such as two-dimensional electrophoresis methods,quantitative mass spectrometry-based techniques or protein microarrays.Additionally,we will also focus on the diverse biological samples used for CRC biomarker discovery such as tissue,serum and faeces,besides cell lines and murine models,discussing their advantages and disadvantages,and summarize the most frequently identified candidate CRC markers.展开更多
文摘【目的】在德国鸢尾的组培过程中,内生菌引起的污染制约着其进行快速繁殖工厂化育苗,针对引起德国鸢尾组培苗污染的内生菌进行分离、鉴定,研究比较6种常用药剂对该菌株的抑制效果,筛选出最佳防治药剂,为德国鸢尾组培快繁过程中有效控制内生菌污染提供科学依据。【方法】采用NA培养基分离纯化菌株(115325),通过形态特征观察、生理生化试验及16S r DNA序列同源性分析,对其进行分类鉴定。采用纸碟抑菌圈法测定6种常用药剂对供试菌株的抑制效果。【结果】供试菌株115325的形态特征,及生理生化指标与表皮葡萄球菌(Staphylococcus epidermidis)基本相同。16S r DNA序列分析表明,该菌株与解糖葡萄球菌S.saccharolyticus(L37602)进化关系最为密切,聚在同一个系统发育分支,与表皮葡萄球菌的同源性最高,为99.86%。室内药剂筛选结果表明,头孢菌素和庆大霉素对供试菌株的抑制作用最强。【结论】综合形态学特征、生理生化特性及16S r DNA序列分析结果,供试菌株115325被鉴定为表皮葡萄球菌。头孢菌素和庆大霉素可作为抑制德国鸢尾组培苗内生菌污染的首选药剂。
基金Supported by Xunta de Galicia(10PXIB310215PR),REGICC(CN 2012/217)Fundación Científica de la Asociación Espaola contra el Cáncer(GCB13131592CAST)+1 种基金Fondo de Investigacio-nes Sanitarias(PI12/00117)FEDER founding
文摘Colorectal cancer(CRC)is the second most common cause of cancer-related deaths in Europe and other Western countries,mainly due to the lack of wellvalidated clinically useful biomarkers with enough sensitivity and specificity to detect this disease at early stages.Although it is well known that the pathogenesis of CRC is a progressive accumulation of mutations in multiple genes,much less is known at the proteome level.Therefore,in the last years many proteomic studies have been conducted to find new candidate protein biomarkers for diagnosis,prognosis and as therapeutic targets for this malignancy,as well as to elucidate the molecular mechanisms of colorectal carcinogenesis.An important advantage of the proteomic approaches is the capacity to look for multiple differentially expressed proteins in a single study.This review provides an overview of the recent reports describing the different proteomic tools used for the discovery of new protein markers for CRC such as two-dimensional electrophoresis methods,quantitative mass spectrometry-based techniques or protein microarrays.Additionally,we will also focus on the diverse biological samples used for CRC biomarker discovery such as tissue,serum and faeces,besides cell lines and murine models,discussing their advantages and disadvantages,and summarize the most frequently identified candidate CRC markers.
