针对炮尾加工工艺复杂且传统工艺规程不能直观反映加工工艺路线等问题,提出一种基于炮尾加工工艺路线专家系统的开发方法.在分析炮尾加工路线的基础上建立加工工艺路线知识库,对其加工工艺进行规律性推理,实现专家系统开发必需的知识库...针对炮尾加工工艺复杂且传统工艺规程不能直观反映加工工艺路线等问题,提出一种基于炮尾加工工艺路线专家系统的开发方法.在分析炮尾加工路线的基础上建立加工工艺路线知识库,对其加工工艺进行规律性推理,实现专家系统开发必需的知识库和推理机,并利用专家系统开发工具DEST(Design Expert System Tools)实现炮尾工艺路线设计专家系统的开发.展开更多
热不对称交错PCR(TAIL-PCR)是检测转基因作物目的基因插入位点的主要技术。为探索适宜大豆的TAIL-PCR反应体系,采用单因素试验和L16(45)正交试验对影响转基因大豆TAIL-PCR的主要参数进行分析,以期建立成熟的大豆TAIL-PCR技术体系。试验...热不对称交错PCR(TAIL-PCR)是检测转基因作物目的基因插入位点的主要技术。为探索适宜大豆的TAIL-PCR反应体系,采用单因素试验和L16(45)正交试验对影响转基因大豆TAIL-PCR的主要参数进行分析,以期建立成熟的大豆TAIL-PCR技术体系。试验设计3个嵌套特异性引物和5种兼并引物进行了3次巢式的热不对称PCR反应。结果表明:退火温度、酶用量、模板浓度、兼并引物和较低温特异性循环在不同水平对TAIL-PCR反应结果均有影响,优化的TAIL-PCR反应体系,即第2次反应为退火温度61℃,1.0 U/30 m L Taq酶,模板浓度30 ng/μL;第3次反应为退火温度62℃,0.5 U/50m L Taq酶,模板浓度40 ng/μL,反应过程中采用降低5个较低温特异性循环,兼并引物采用AD3,在此条件下扩增的条带清晰、稳定、特异性好,适合大豆TAIL-PCR反应体系。展开更多
A video system for tracking a free-swimming fish two-dimensionally is introduced in this paper. The tracking is accomplished by simultaneously taking images from the ventral view and the lateral view of the fish with ...A video system for tracking a free-swimming fish two-dimensionally is introduced in this paper. The tracking is accomplished by simultaneously taking images from the ventral view and the lateral view of the fish with two CCD cameras mounted on two computer-controlled and mutually orthogonal translation stages. By processing the images recorded during tracking,three-dimensional kinematic parameters of the tail and pectoral fin of the fish in forward,backward and turning swimming modes are obtained.展开更多
With the aim to apply the electric fish into practice to assist coal mine water disaster life detection and rescue work, based on the analysis on swing propulsion movements of tail fin, this paper integrates the elect...With the aim to apply the electric fish into practice to assist coal mine water disaster life detection and rescue work, based on the analysis on swing propulsion movements of tail fin, this paper integrates the electromagnet technology with tail fin drive system by analyzing how the fish swims with tail fin under the law of progression. The principle, structure, and drive signals of tail fin electromagnetic drive are researched, the enforced situation of fish under eIectromagnetic driving modes are analyzed, and the experimental plat-form of tail fin electromagnetic drive is established. The best distance between electro- magnet and armature, which can realize the swing of tail fin, was researched in the experiment under water. The robotic fish structure parameters of tail fin electromagnetic drive was finalized by theoretical analysis and experimental measurement.展开更多
文摘针对炮尾加工工艺复杂且传统工艺规程不能直观反映加工工艺路线等问题,提出一种基于炮尾加工工艺路线专家系统的开发方法.在分析炮尾加工路线的基础上建立加工工艺路线知识库,对其加工工艺进行规律性推理,实现专家系统开发必需的知识库和推理机,并利用专家系统开发工具DEST(Design Expert System Tools)实现炮尾工艺路线设计专家系统的开发.
文摘热不对称交错PCR(TAIL-PCR)是检测转基因作物目的基因插入位点的主要技术。为探索适宜大豆的TAIL-PCR反应体系,采用单因素试验和L16(45)正交试验对影响转基因大豆TAIL-PCR的主要参数进行分析,以期建立成熟的大豆TAIL-PCR技术体系。试验设计3个嵌套特异性引物和5种兼并引物进行了3次巢式的热不对称PCR反应。结果表明:退火温度、酶用量、模板浓度、兼并引物和较低温特异性循环在不同水平对TAIL-PCR反应结果均有影响,优化的TAIL-PCR反应体系,即第2次反应为退火温度61℃,1.0 U/30 m L Taq酶,模板浓度30 ng/μL;第3次反应为退火温度62℃,0.5 U/50m L Taq酶,模板浓度40 ng/μL,反应过程中采用降低5个较低温特异性循环,兼并引物采用AD3,在此条件下扩增的条带清晰、稳定、特异性好,适合大豆TAIL-PCR反应体系。
基金Supported by the National Natural Science Foundation of China (Grant No. 10332040)
文摘A video system for tracking a free-swimming fish two-dimensionally is introduced in this paper. The tracking is accomplished by simultaneously taking images from the ventral view and the lateral view of the fish with two CCD cameras mounted on two computer-controlled and mutually orthogonal translation stages. By processing the images recorded during tracking,three-dimensional kinematic parameters of the tail and pectoral fin of the fish in forward,backward and turning swimming modes are obtained.
文摘With the aim to apply the electric fish into practice to assist coal mine water disaster life detection and rescue work, based on the analysis on swing propulsion movements of tail fin, this paper integrates the electromagnet technology with tail fin drive system by analyzing how the fish swims with tail fin under the law of progression. The principle, structure, and drive signals of tail fin electromagnetic drive are researched, the enforced situation of fish under eIectromagnetic driving modes are analyzed, and the experimental plat-form of tail fin electromagnetic drive is established. The best distance between electro- magnet and armature, which can realize the swing of tail fin, was researched in the experiment under water. The robotic fish structure parameters of tail fin electromagnetic drive was finalized by theoretical analysis and experimental measurement.
