Seeds of Rhynchostylis retusa (L.) Bl. germinate faster on the KC medium than on the VW medium. Protocorms of R. retusa were successfully encapsulated in the KC solution (containing 3% sodium alginate) to get syntheti...Seeds of Rhynchostylis retusa (L.) Bl. germinate faster on the KC medium than on the VW medium. Protocorms of R. retusa were successfully encapsulated in the KC solution (containing 3% sodium alginate) to get synthetic seeds and germination rate of synthetic seeds stored at 4℃ was higher than at 20℃ and 25℃ on the KC medium containing 15% coconut milk, 2 g·L -1 peptone and 1 mg·L -1 NAA. Time taken for germination will be longer and germination rate will decrease at different storage temperatures with the increasing of storage time. Survival rates of seedlings from the synthetic seeds stored at 4℃ for 15, 30, 45, 60 and 75 d are 93%, 89%, 80%, 75% and 63% on field transfer, respectively.展开更多
Zephyranthes is valued as a native ornamental landscaping plant and a traditional medicinal herb. Due to the low seed viability, this study was carried out to evaluate the potential of seed embryo rescue using the art...Zephyranthes is valued as a native ornamental landscaping plant and a traditional medicinal herb. Due to the low seed viability, this study was carried out to evaluate the potential of seed embryo rescue using the artificial seed technique and long-preservation in Z. atamasca and Z. grandiflora. Seed embryos were selected for encapsulation with different concentration of sodium alginate (3%, 4%, and 5%) and calcium chloride (either 25, 50, 75, and 100 mM) followed by no encapsulated embryos as a control. The greatest viability of encapsulated embryos was 95% in Z. grandiflora and 85% in Z. atamasca with the combination of 4% sodium alginate and 100 mM calcium chloride after two weeks at 5°C. The highest viability with A<sub>490nm</sub>0.12 and A<sub>490nm</sub>0.16 were achieved when embryos were cultured in pretreatment medium with 30 g/L sucrose in Z. grandiflora and Z. atamasca, respectively. The highest viability by TTC assay after cryopreservation was observed with 54% viability for Z. grandiflora and 48% viability with Z. atamasca, after 2 h of dehydration. Rain lilies embryos were successfully preserved functioned as artificial seed and cryopreservation using encapsulation-dehydration method has been established for both species that can be used for other flower species with some modifications.展开更多
文摘Seeds of Rhynchostylis retusa (L.) Bl. germinate faster on the KC medium than on the VW medium. Protocorms of R. retusa were successfully encapsulated in the KC solution (containing 3% sodium alginate) to get synthetic seeds and germination rate of synthetic seeds stored at 4℃ was higher than at 20℃ and 25℃ on the KC medium containing 15% coconut milk, 2 g·L -1 peptone and 1 mg·L -1 NAA. Time taken for germination will be longer and germination rate will decrease at different storage temperatures with the increasing of storage time. Survival rates of seedlings from the synthetic seeds stored at 4℃ for 15, 30, 45, 60 and 75 d are 93%, 89%, 80%, 75% and 63% on field transfer, respectively.
文摘Zephyranthes is valued as a native ornamental landscaping plant and a traditional medicinal herb. Due to the low seed viability, this study was carried out to evaluate the potential of seed embryo rescue using the artificial seed technique and long-preservation in Z. atamasca and Z. grandiflora. Seed embryos were selected for encapsulation with different concentration of sodium alginate (3%, 4%, and 5%) and calcium chloride (either 25, 50, 75, and 100 mM) followed by no encapsulated embryos as a control. The greatest viability of encapsulated embryos was 95% in Z. grandiflora and 85% in Z. atamasca with the combination of 4% sodium alginate and 100 mM calcium chloride after two weeks at 5°C. The highest viability with A<sub>490nm</sub>0.12 and A<sub>490nm</sub>0.16 were achieved when embryos were cultured in pretreatment medium with 30 g/L sucrose in Z. grandiflora and Z. atamasca, respectively. The highest viability by TTC assay after cryopreservation was observed with 54% viability for Z. grandiflora and 48% viability with Z. atamasca, after 2 h of dehydration. Rain lilies embryos were successfully preserved functioned as artificial seed and cryopreservation using encapsulation-dehydration method has been established for both species that can be used for other flower species with some modifications.
