[Objectives]To explore the expression of interleukin 34(IL-34)in gastric adenocarcinoma tissues and its relationship with apoptosis of gastric adenocarcinoma cells.[Methods]60 cases of surgically resected human gastri...[Objectives]To explore the expression of interleukin 34(IL-34)in gastric adenocarcinoma tissues and its relationship with apoptosis of gastric adenocarcinoma cells.[Methods]60 cases of surgically resected human gastric adenocarcinoma tissue specimen and 50 cases of adjacent normal gastric mucosa tissue specimen were collected,and the expression of IL-34 protein was determined by immunohistochemical streptavidin-perosidase(SP)method.Cell apoptosis in tissue specimen was detected by TUNEL staining method,and the relationship between IL-34 protein expression and apoptosis of gastric adenocarcinoma cells was analyzed.[Results]Immunohistochemical SP experiment indicated that the expression of IL-34 protein in gastric adenocarcinoma was higher than that in adjacent normal gastric mucosa(P<0.05);its positive expression was related to histological differentiation,TNM stage,invasion depth,and lymph node metastasis of gastric adenocarcinoma(P<0.05),but not to gender and age(P>0.05).TUNEL experiment showed that compared with the adjacent normal gastric mucosa group,the apoptosis index(AI)of gastric adenocarcinoma group was significantly lower(P<0.05);the AI was related to histological differentiation,TNM stage,tumor invasion depth and lymph node metastasis of gastric adenocarcinoma(P<0.05),but not to gender and age(P>0.05).In gastric adenocarcinoma,the AI of IL-34 positive expression group was lower than that of IL-34 negative expression group,and the results were statistically significant(P<0.05).[Conclusions]IL-34 has high expression in gastric adenocarcinoma tissues and is negatively correlated with cancer cell apoptosis.Abnormal expression of IL-34 is involved in the occurrence and development of gastric adenocarcinoma.This provides a new idea for the pathogenesis research and clinical treatment of gastric adenocarcinoma.展开更多
基金Supported by Cultivation Fund of National Natural Science Foundation of China Project(202114)Major Program at School Level(201711).
文摘[Objectives]To explore the expression of interleukin 34(IL-34)in gastric adenocarcinoma tissues and its relationship with apoptosis of gastric adenocarcinoma cells.[Methods]60 cases of surgically resected human gastric adenocarcinoma tissue specimen and 50 cases of adjacent normal gastric mucosa tissue specimen were collected,and the expression of IL-34 protein was determined by immunohistochemical streptavidin-perosidase(SP)method.Cell apoptosis in tissue specimen was detected by TUNEL staining method,and the relationship between IL-34 protein expression and apoptosis of gastric adenocarcinoma cells was analyzed.[Results]Immunohistochemical SP experiment indicated that the expression of IL-34 protein in gastric adenocarcinoma was higher than that in adjacent normal gastric mucosa(P<0.05);its positive expression was related to histological differentiation,TNM stage,invasion depth,and lymph node metastasis of gastric adenocarcinoma(P<0.05),but not to gender and age(P>0.05).TUNEL experiment showed that compared with the adjacent normal gastric mucosa group,the apoptosis index(AI)of gastric adenocarcinoma group was significantly lower(P<0.05);the AI was related to histological differentiation,TNM stage,tumor invasion depth and lymph node metastasis of gastric adenocarcinoma(P<0.05),but not to gender and age(P>0.05).In gastric adenocarcinoma,the AI of IL-34 positive expression group was lower than that of IL-34 negative expression group,and the results were statistically significant(P<0.05).[Conclusions]IL-34 has high expression in gastric adenocarcinoma tissues and is negatively correlated with cancer cell apoptosis.Abnormal expression of IL-34 is involved in the occurrence and development of gastric adenocarcinoma.This provides a new idea for the pathogenesis research and clinical treatment of gastric adenocarcinoma.
