摘要
目的:研究胰岛素样生长因子ⅡmRNA结合蛋白Ⅲ(IMP-3)在不同病变子宫内膜组织中的表达,探讨其在子宫内膜病变发生过程中的作用机制。方法:应用免疫组织化学链霉菌抗生物素蛋白-过氧化酶连接法(SP法)检测20例正常子宫内膜组织、10例不典型增生内膜组织及40例子宫内样腺癌组织中IMP-3的表达情况,分析其表达量与子宫内膜癌临床病例特征之间的关系。结果:IMP-3在正常子宫内膜组织、不典型增生内膜组织及子宫内样腺癌组织中的相对表达量分别为5.361±1.074、13.587±2.301和19.572±1.936,两两间表达差异有统计学意义(P<0.05);在不同手术-临床分期和病理组织学分级的子宫内膜癌组织中,IMP-3的表达量有统计学差异(P<0.05)。结论:IMP-3可能参与调节子宫内膜病变的发生发展过程。
[Abstract] Objective： To detect the expressions of insulin-like growth factor Ⅱ mRNA-binding protein Ⅲ （IMP-3） in different endometrial tissues and explore the relationship between IMP-3 and the development of endometrial carcinoma. Methods： The expressions of IMP-3 in 40 cases of endometrial carcinoma, 10 cases of atypical hyper- plasia endometrial tissue and 20 cases of normal endometrial tissues were examined by streptavidin-perosidase （SP） method. Results： Compared with the normal and atypical hyperplasia endometrial endometrial tissues, there were significant differences in the expression of IMP-3 protein in endometrial carcinoma tissues（P〈0.05）, and the expression in endometrial cancer tissues was significantly different associated with the pathological grading and stag- ing. Conclusion： IMP-3 protein may be involved in the process of endometrial lesions.
出处
《生物技术通讯》
CAS
2013年第1期91-93,共3页
Letters in Biotechnology
基金
辽宁省自然科学基金(20092127)
关键词
子宫内膜癌
胰岛素样生长因子Ⅱ
mRNA结合蛋白Ⅲ
免疫组织化学
SP法
endometrial carcinoma
insulin-like growth factor Ⅱ mRNA-binding protein Ⅲ
immunohistochemis- try
streptavidin-perosidase method
